Repeated inoculation with rumen fluid accelerates the rumen bacterial transition with no benefit on production performance in postpartum Holstein dairy cows

Background The dairy cow’s postpartum period is characterized by dramatic physiological changes,therefore imposing severe challenges on the animal for maintaining health and milk output.The dynamics of the ruminal microbiota are also tremendous and may play a crucial role in lactation launch.We aim to investigate the potential benefits of early microbial intervention by fresh rumen microbiota transplantation(RMT)and sterile RMT in postpartum dairy cows.Twelve fistulated peak-lactation dairy cows were selected to be the donors for rumen fluid collection.Thirty postpartum cows were divided into 3 groups as the transplantation receptors respectively receiving 10 L fresh rumen fluid(FR),10 L sterile rumen fluid(SR),or 10 L saline(CON)during 3 d after calving.Results Production performance,plasma indices,plasma lipidome,ruminal microbiome,and liver transcriptome were recorded.After fresh and sterile RMT,we found that the molar proportion of propionic acid was increased on d 7 in the FR and SR groups and the bacterial composition was also significantly changed when compared with the CON group.A similarity analysis showed that the similarities between the CON group and FR or SR group on d 7 were 48.40%or 47.85%,whereas the similarities between microbiota on d 7 and 21 in the FR and SR groups were 68.34%or 66.85%.Dry matter intake and feed efficiency were not affected by treatments.Plasmaβ-hydroxybutyrate concentration in the FR group was decreased and significantly different lipids mainly included phosphatidylcholine and lysophosphatidylcholine containing polyunsaturated fatty acids.Hepatic transcriptomics analysis indicated acutephase response pathways were upregulated in the SR group.Conclusions Our study suggests that RMT can shorten the transition process of the ruminal microbiota of postpartum dairy cows with no benefit on dry matter intake or feed efficiency.Inoculation with rumen fluid may not be a useful approach to promote the recovery of postpartum dairy cows.

Enhancement of spatial resolution of ghost imaging via localizing and thresholding

In ghost imaging, an illumination light is split into test and reference beams which pass through two different optical systems respectively and an image is constructed with the second-order correlation between the two light beams. Since both light beams are diffracted when passing through the optical systems, the spatial resolution of ghost imaging is in general lower than that of a corresponding conventional imaging system. When Gaussian-shaped light spots are used to illuminate an object, randomly scanning across the object plane, in the ghost imaging scheme, we show th√at by localizing central positions of the spots of the reference light beam, the resolution can be increased by a factor of 2^(1/2) same as that of the corresponding conventional imaging system. We also find that the resolution can be further enhanced by setting an appropriate threshold to the bucket measurement of ghost imaging.

Effect of fermented bee pollen on metabolic syndrome in high-fat diet-induced mice

Bee pollen has potential in preventing metabolic syndrome(MetS).The present study aimed to investigate the effect of yeast-fermented wall-broken bee pollen(YB)intervention on ICR mice with MetS induced with a high-fat(HF)diet.After YB intervention in mice for 16 weeks,the effect on alleviating MetS was evaluated based on MetS serum parameters,hepatic oxidant status markers and gut microbial populations.The results of animal experiment showed that YB intervention attenuated MetS.Based on multivariate statistical analysis results,YB treatment signifi cantly increased glutathione S-transferase(GST)and catalase(CAT)activities and decreased the malondialdehyde(MDA)level in the liver.Further investigation showed that YB restored the Nrf-2-Keap-1 pathway to alleviate oxidative stress.Additionally,gut microbial community analysis revealed that YB restored the increase in the Firmicutes to Bacteroidetes(F/B)ratio(6.94 for the HF group and 3.74 for HF+YB group)and improved Lactobacillus and Lactococcus abundance induced by the HF diet.Overall,YB improved function and prevented MetS by modulating the gut microbiota and alleviating oxidative stress.

A Smoothing Neural Network Algorithm for Absolute Value Equations

In this paper, we give a smoothing neural network algorithm for absolute value equations (AVE). By using smoothing function, we reformulate the AVE as a differentiable unconstrained optimization and we establish a steep descent method to solve it. We prove the stability and the equilibrium state of the neural network to be a solution of the AVE. The numerical tests show the efficient of the proposed algorithm.

Spectral Gradient Algorithm Based on the Generalized Fiser-Burmeister Function for Sparse Solutions of LCPS

This paper considers the computation of sparse solutions of the linear complementarity problems LCP(q, M). Mathematically, the underlying model is NP-hard in general. Thus an lp(0 p < 1) regularized minimization model is proposed for relaxation. We establish the equivalent unconstrained minimization reformation of the NCP-function. Based on the generalized Fiser-Burmeister function, a sequential smoothing spectral gradient method is proposed to solve the equivalent problem. Numerical results are given to show the efficiency of the proposed method.

聚偏氟乙烯膜三维离子传输通道的构建及其在全钒液流电池中的性能研究

随着新能源技术的不断发展,大规模储能技术受到了广泛的关注.全钒液流电池因其容量和功率设计灵活、安全可靠、寿命长等优势成为发展较快的储能电池之一.离子膜作为液流电池的关键部件,对电池的能量转化效率、寿命和成本具有显著影响.本工作以高化学稳定性的聚偏氟乙烯作为膜材料,利用聚乙二醇和聚乙烯吡咯烷酮分别作为模板和稳定剂,在聚偏氟乙烯膜内成功构建了具有较好H/V离子选择性的三维离子传输通道.电池性能测试表明,该聚偏氟乙烯(PVDF)离子膜有着出色的化学稳定性,在100m A·cm^(-2)电流密度下,具有98%以上的电流效率和83.5%的能量效率.此外,聚偏氟乙烯具有价格低的显著特点,使其在全钒液流电池中有较好的应用前景.

全钒液流电池离子导电膜

随着可再生能源技术的不断发展,全钒液流电池作为具有较大发展前景的大规模储能装置,受到了国内外的广泛关注。离子导电膜作为全钒液流电池重要的组成部件之一,对于电池的性能、使用寿命和成本有着关键性的影响。根据国内外的研究报道,本综述详细介绍了全钒液流电池离子导电膜的科研与应用进展以及所面临的技术难题,为高性能、低成本、长寿命的离子导电膜的开发,促进全钒液流电池商业化利用提供了参考依据并对未来的研究方向进行了展望。