The presence of sperm was observed in different organs of the reproductive tract of M. schmitti. These were the club, baffle and terminal zone of the oviducal gland, posterior portion of the uterus and in the cervix. ...The presence of sperm was observed in different organs of the reproductive tract of M. schmitti. These were the club, baffle and terminal zone of the oviducal gland, posterior portion of the uterus and in the cervix. This fact allowed for consideration not only its storage but its interaction with the female reproductive tract epithelia as well. SEM （microscopic observation studies） detected loose and sole spermatozoa in the club and baffle zones in a pregnant female. On the other hand, histochemical techniques showed sperm storage tubules of the terminal zone, which contain bundles of sperm, do not stain AB （alcian blue） or PAS （periodic acid schiff）. Disintegration of some of the male gametes was observed in the posterior part of the uterus with TEM （transmission electron microscopy） studies which would indicate a first uterine sperm selection. The uterine epithelial cells involved in this process showed an important level of secretion vacuoles suggesting an interaction with sperm cells. Sperm in the cervix was found without the matrix that constitutes the spermatozeugmata formed in the male tract. Some heads of the sperm were uncurled while they were interacting with the cervical cells.展开更多
Moina eugeniae is the most abundant species in the southwest lagoons of Buenos Aires province. The aim of this work is to study the histology of this cladoceran by light microscopy so as to expand the knowledge of thi...Moina eugeniae is the most abundant species in the southwest lagoons of Buenos Aires province. The aim of this work is to study the histology of this cladoceran by light microscopy so as to expand the knowledge of this species. The parthenogenetic females were fixed in formaldehyde 4%. Sections were cut 3 μm thick and stained with Hematoxylin and Eosin. The intestine has simple cuboidal epithelium with apical projections and the cells have 1 or 2 nucleoli in their nuclei. Two compound eyes were found and no naupliar ocelum. The ovary is saccular and it is presented in a pair at both sides of the intestine with follicles in different states of development. The striated muscle with notorious microfibrills is recognized in antennas, antennules and trunk appendices. This article may represent the first detailed description of the histology of this species.展开更多
文摘The presence of sperm was observed in different organs of the reproductive tract of M. schmitti. These were the club, baffle and terminal zone of the oviducal gland, posterior portion of the uterus and in the cervix. This fact allowed for consideration not only its storage but its interaction with the female reproductive tract epithelia as well. SEM （microscopic observation studies） detected loose and sole spermatozoa in the club and baffle zones in a pregnant female. On the other hand, histochemical techniques showed sperm storage tubules of the terminal zone, which contain bundles of sperm, do not stain AB （alcian blue） or PAS （periodic acid schiff）. Disintegration of some of the male gametes was observed in the posterior part of the uterus with TEM （transmission electron microscopy） studies which would indicate a first uterine sperm selection. The uterine epithelial cells involved in this process showed an important level of secretion vacuoles suggesting an interaction with sperm cells. Sperm in the cervix was found without the matrix that constitutes the spermatozeugmata formed in the male tract. Some heads of the sperm were uncurled while they were interacting with the cervical cells.
文摘Moina eugeniae is the most abundant species in the southwest lagoons of Buenos Aires province. The aim of this work is to study the histology of this cladoceran by light microscopy so as to expand the knowledge of this species. The parthenogenetic females were fixed in formaldehyde 4%. Sections were cut 3 μm thick and stained with Hematoxylin and Eosin. The intestine has simple cuboidal epithelium with apical projections and the cells have 1 or 2 nucleoli in their nuclei. Two compound eyes were found and no naupliar ocelum. The ovary is saccular and it is presented in a pair at both sides of the intestine with follicles in different states of development. The striated muscle with notorious microfibrills is recognized in antennas, antennules and trunk appendices. This article may represent the first detailed description of the histology of this species.
