Cerebellar malfunction can lead to sleep disturbance such as excessive daytime sleepiness,suggesting that the cerebellum may be involved in regulating sleep and/or wakefulness.However,understanding the features of cer...Cerebellar malfunction can lead to sleep disturbance such as excessive daytime sleepiness,suggesting that the cerebellum may be involved in regulating sleep and/or wakefulness.However,understanding the features of cerebellar regulation in sleep and wakefulness states requires a detailed characterization of neuronal activity within this area.By performing multiple-unit recordings in mice,we showed that Purkinje cells(PCs)in the cerebellar cortex exhibited increased firing activity prior to the transition from sleep to wakefulness.Notably,the increased PC activity resulted from the inputs of lowfrequency non-PC units in the cerebellar cortex.Moreover,the increased PC activity was accompanied by decreased activity in neurons of the deep cerebellar nuclei at the nonrapid eye-movement sleep-wakefulness transition.Our results provide in vivo electrophysiological evidence that the cerebellum has the potential to actively regulate the sleep-wakefulness transition.展开更多
基金supported by grants from the National Natural Science Foundation of China (81671315)the Natural Science Foundation of Chongqing Municipality (cstc2019jcyj-msxmX0424)+1 种基金the Frontier Interdisciplinary Project of the College of Basic Sciences,Army Medical University,China (2018JCQY01)the National Training Program of Innovation and Entrepreneurship for Undergraduates,China (201990035020)。
文摘Cerebellar malfunction can lead to sleep disturbance such as excessive daytime sleepiness,suggesting that the cerebellum may be involved in regulating sleep and/or wakefulness.However,understanding the features of cerebellar regulation in sleep and wakefulness states requires a detailed characterization of neuronal activity within this area.By performing multiple-unit recordings in mice,we showed that Purkinje cells(PCs)in the cerebellar cortex exhibited increased firing activity prior to the transition from sleep to wakefulness.Notably,the increased PC activity resulted from the inputs of lowfrequency non-PC units in the cerebellar cortex.Moreover,the increased PC activity was accompanied by decreased activity in neurons of the deep cerebellar nuclei at the nonrapid eye-movement sleep-wakefulness transition.Our results provide in vivo electrophysiological evidence that the cerebellum has the potential to actively regulate the sleep-wakefulness transition.
