To understand virus infection situation of integrated demonstration and regional test sugarcane varieties in national sugarcane system in Guilin City,Guangxi Province,leaf samples with or without symptoms were collect...To understand virus infection situation of integrated demonstration and regional test sugarcane varieties in national sugarcane system in Guilin City,Guangxi Province,leaf samples with or without symptoms were collected from 37 sugarcane varieties,and five kinds of viruses were detected by RT-PCR with specific primers,including Sugarcane yellow leaf virus( SCYLV),Sugarcane streak mosaic virus( SCSMV),Sorghum mosaic virus( SrM V),Sugarcane mosaic virus( SCMV) and Sugarcane bacilliform virus( SCBV). The results showed that SCYLV was detected from four sugarcane varieties,and the detection rate was10. 81%; SCSMV was detected from five sugarcane varieties,and the detection rate was 13. 51%; SrM V was detected from four sugarcane varieties,and the detection rate was 10. 81%; SCMV had not been found; SCBV was detected from 27 sugarcane varieties,and the detection rate was 72. 9%. Additionally,mixed infection of different viruses was widespread,and the total mixed infection rate was 21. 62%. Thus,integrated demonstration and regional test sugarcane varieties in national sugarcane system in Guilin City had been seriously infected by viruses,and mixed infection of different viruses was common. The paper will provide a reference for breeding and popularization of antiviral sugarcane varieties.展开更多
[Objective]The paper was to clarify the occurrence of sugarcane ratoon stunting disease(RSD)in Hainan sugarcane-growing areas.[Method]In 2019,270 samples of sugarcane leaves were collected from six main sugarcane-grow...[Objective]The paper was to clarify the occurrence of sugarcane ratoon stunting disease(RSD)in Hainan sugarcane-growing areas.[Method]In 2019,270 samples of sugarcane leaves were collected from six main sugarcane-growing areas in Hainan Province,and RSD was detected by PCR assay with specific primers.[Result]RSD was detected out in 41 out of 270 sugarcane samples,with an average detection rate of15.19%.The detection rates of RSD were different in six sugarcane-growing areas;the detection rate of RSD in Danzhou sugarcane-growing area was the highest of 22.00%;the detection rate of RSD in Lingao sugarcane-growing area was the lowest of 9.26%.RSD was detected out in 8 out of10 main sugarcane cultivars,among which Xintaitang 22 suffered the heaviest damage,with the positive detection rate of 45.83%;RSD had not been detected out in Zhongtang 1 and Zhongtang 2,while the positive detection rates of RSD in the remaining seven sugarcane cultivars were10.00%-31.25%.[Conclusion]RSD commonly occurs in Hainan sugarcane-growing areas.The research results provide a basis for scientific prevention and control of RSD and promotion and application of healthy virus-free sugarcane seedlings.展开更多
The present study was to screen and identify a biocontrol strain with antagonistic effect against Sporisorium scitaminea Sydow. 16 S rDNA gene fragments were amplified by universal primers P0 and P6. The results showe...The present study was to screen and identify a biocontrol strain with antagonistic effect against Sporisorium scitaminea Sydow. 16 S rDNA gene fragments were amplified by universal primers P0 and P6. The results showed that 16 S rDNA gene fragment with the size of 1 533 bp was obtained,and its sequence had the homology of 99% with the sequence of Bacillus subtilis. The morphological characteristics of the strain were as follows： the mycelium of strain HAS was short-rod shape,and could move; Gram staining was positive; 3% KOH solubility test was negative; flagellum was grown around the cells; spores were grown in the middle,elliptic,and cysts were slightly swelled. The strain was preliminarily identified as B. subtilis. Dual culture with pathogenic fungi showed that the strain had good inhibitory effect against various pathogenic fungi causing diseases on sugarcane and other crops. Therefore,strain HAS isolated in the study was identified to be B.subtilis,which had good control effect against S. scitaminea.展开更多
基金Supported by Special Fund of China Agricultural(Sugar)Industry Research System(CARS-170301)Basal Research Fund of Central Public Welfare Research Institutes(1630052016010)
文摘To understand virus infection situation of integrated demonstration and regional test sugarcane varieties in national sugarcane system in Guilin City,Guangxi Province,leaf samples with or without symptoms were collected from 37 sugarcane varieties,and five kinds of viruses were detected by RT-PCR with specific primers,including Sugarcane yellow leaf virus( SCYLV),Sugarcane streak mosaic virus( SCSMV),Sorghum mosaic virus( SrM V),Sugarcane mosaic virus( SCMV) and Sugarcane bacilliform virus( SCBV). The results showed that SCYLV was detected from four sugarcane varieties,and the detection rate was10. 81%; SCSMV was detected from five sugarcane varieties,and the detection rate was 13. 51%; SrM V was detected from four sugarcane varieties,and the detection rate was 10. 81%; SCMV had not been found; SCBV was detected from 27 sugarcane varieties,and the detection rate was 72. 9%. Additionally,mixed infection of different viruses was widespread,and the total mixed infection rate was 21. 62%. Thus,integrated demonstration and regional test sugarcane varieties in national sugarcane system in Guilin City had been seriously infected by viruses,and mixed infection of different viruses was common. The paper will provide a reference for breeding and popularization of antiviral sugarcane varieties.
基金Supported by National Natural Science Foundation of China(31771865)National Sugar Industry Technology System"Scientists for Prevention and Control of Sugarcane Virus Diseases"(CARS-170301)。
文摘[Objective]The paper was to clarify the occurrence of sugarcane ratoon stunting disease(RSD)in Hainan sugarcane-growing areas.[Method]In 2019,270 samples of sugarcane leaves were collected from six main sugarcane-growing areas in Hainan Province,and RSD was detected by PCR assay with specific primers.[Result]RSD was detected out in 41 out of 270 sugarcane samples,with an average detection rate of15.19%.The detection rates of RSD were different in six sugarcane-growing areas;the detection rate of RSD in Danzhou sugarcane-growing area was the highest of 22.00%;the detection rate of RSD in Lingao sugarcane-growing area was the lowest of 9.26%.RSD was detected out in 8 out of10 main sugarcane cultivars,among which Xintaitang 22 suffered the heaviest damage,with the positive detection rate of 45.83%;RSD had not been detected out in Zhongtang 1 and Zhongtang 2,while the positive detection rates of RSD in the remaining seven sugarcane cultivars were10.00%-31.25%.[Conclusion]RSD commonly occurs in Hainan sugarcane-growing areas.The research results provide a basis for scientific prevention and control of RSD and promotion and application of healthy virus-free sugarcane seedlings.
基金Supported by Natural Science Foundation of Hainan Province(314120)Fundamental Research Fund for Central Public-interest Scientific Institutions(ITBB130502)Special Fund of China Agricultural Industry Research Sys-tem(nycytx-24)
文摘The present study was to screen and identify a biocontrol strain with antagonistic effect against Sporisorium scitaminea Sydow. 16 S rDNA gene fragments were amplified by universal primers P0 and P6. The results showed that 16 S rDNA gene fragment with the size of 1 533 bp was obtained,and its sequence had the homology of 99% with the sequence of Bacillus subtilis. The morphological characteristics of the strain were as follows： the mycelium of strain HAS was short-rod shape,and could move; Gram staining was positive; 3% KOH solubility test was negative; flagellum was grown around the cells; spores were grown in the middle,elliptic,and cysts were slightly swelled. The strain was preliminarily identified as B. subtilis. Dual culture with pathogenic fungi showed that the strain had good inhibitory effect against various pathogenic fungi causing diseases on sugarcane and other crops. Therefore,strain HAS isolated in the study was identified to be B.subtilis,which had good control effect against S. scitaminea.
