中烟特香301特异SNP指纹图谱构建及应用

指纹图谱可以用于品种真实性鉴定,为品种的推广种植和工业利用提供技术保障。本研究采用全基因组重测序技术,对包括烤烟新品种中烟特香301在内的33份烟草种质资源进行了单核苷酸多态性(SNP)变异分析,从中鉴定获得中烟特香301特异SNP位点268个。利用包括烤烟、雪茄烟、香料烟、白肋烟、晒烟在内的62份不同类型烟草种质资源进行了筛选,初步获得有效SNP标记21个。以2022—2023年山东产区的中烟特香301及其他5个主栽品种的大田新鲜烟叶和初烤烟叶为材料,利用上述21个SNP标记进行品种真实性检测,结果显示,18个SNP标记在所有烟叶中可全部检测到中烟特香301特异SNP位点。利用这18个SNP标记构建的指纹图谱可以用于中烟特香301品种真实性鉴定。

恩诺沙星纳米乳的制备及喷雾给药的药效学评价

【目的】以恩诺沙星为主药,研制一种可喷雾给药的纳米乳新制剂,并通过鸡全身感染疾病模型,对恩诺沙星纳米乳喷雾制剂的药效进行初步评价。【方法】采用相转变法,通过单因素试验和正交试验对辅料进行筛选及处方工艺优化,采用高效液相色谱法(HPLC)对纳米乳中恩诺沙星的含量进行测定,采用纳米粒度及Zeta电位分析仪测定纳米乳粒径大小和Zeta电位,并开展人工诱发鸡白痢沙门氏菌病和禽大肠埃希菌病感染模型的药效学试验,评估恩诺沙星纳米乳喷雾给药对常见细菌性疾病的防治效果。【结果】本试验所制备的纳米乳为水包油型,呈淡黄色,澄清透明,乳滴分布均匀,平均粒径为(10.79±1.5)nm,多分散系数(PDI)为0.446,Zeta电位为−21.63 mV。药效学评估显示:高、中、低剂量预防给药组的存活率分别为90.0%、83.3%、73.3%,均显著高于感染对照组(26.7%)(P<0.01)。【结论】本研究制备的复方恩诺沙星纳米乳性质稳定,超声雾化喷雾给药对沙门氏菌和大肠埃希菌引起的鸡全身感染有良好的防治效果。

检查任务管理系统在企业管理中的应用价值

检查任务管理系统在推动企业精准化、规范化、信息化、高效化管理方面具有较高的价值,其可以有效检查企业在经营活动以及管理实践中存在的问题,助推各部门改进工作,提高整体效率。本文概述了检查任务管理系统,分析了企业检查工作存在的问题以及检查任务管理系统在企业管理中的应用价值,最后给出了应用建议。

嘉靖《湖广图经志书》编纂考略

检阅见存《湖广图经志书》,所载不仅有嘉靖初年之事,更记有嘉靖二十年(1541)以后的内容。比校天一阁藏本和影尊经阁藏本及南京图书馆藏本可知,《湖广图经志书》于嘉靖元年初刻之后,似至少有过三次校改增补,第一次是嘉靖二年吴廷举令教谕吴錬等所做改补,第二次改补的时间不会早于嘉靖十一年,第三次改补则不早于嘉靖二十二年。从卷目及部分内容安排考察,嘉靖《湖广图经志书》已经突破了"姑仍旧志,附以新事"的既定体例,亦未完全保留成化《湖广通志》的内容。

Coronary heart disease： incidence, risk factors and interventions in Jiaozhou of Shandong province

Background Coronary heart disease （CHD） is the most common type of heart disease and cause of heart attacks.This study investigated the epidemiological characteristics of CHD and its risk factors in Jiaozhou,Shandong province,to ultimately find a way of reducing the prevalence of cardiovascular disease,and to provide a theoretical basis for establishing a cardiovascular disease management path under the regional medical collaborative mechanism.Methods A questionnaire survey was performed including 1 952 people aged 35 years or older who were questioned by means of stratified,cluster,proportional sampling to investigate the prevalence of CHD and its risk factors.The data were inputted into SPSS11.0 statistical software for processing and analysis.We advised the local medical institutions to establish health files for the residents with CHD and risk factors.They were followed up regularly.Their risk factors and life-style were monitored,and advice was given as to proper medications.Green channels were established,and the patients were transmitted in a timely manner to superior hospitals for better treatment if the necessary treatments were not available in the local hospitals.The control of risk factors was observed after the follow-up for half a year.Results In Jiaozhou,the rates of coronary artery disease,hypertension,diabetes,hyperlipidemia and overweight were 8.15％,28.54％,11.43％,35.46％,and 18.70％ respectively.The rates of hypertension,diabetes,hyperlipidemia and overweight were higher than the data published in ＂The report of Chinese cardiovascular disease 2012＂; which are 24％,9.7％,18.6％,and 9.7％,respectively.The control of risk factors improved significantly after the guidance of the residents lifestyle and medication for six months.Conclusions The high prevalence of coronary artery disease in Jiaozhou is closely related to age,gender,diet structure,family history of cardiovascular disease,hypertension,diabetes,hyperlipidemia,overweight,and unhealthy lifestyle.Under the regional medical coordination mechanism,the collaborative management of cardiovascular disease can provide new management concepts for the areas short of medical resources,so as to reduce the prevalence of cardiovascular disease.

明清湖北的棉花种植与棉花贸易——以江汉平原为中心

在明代基本普及的基础上,江汉平原的棉花种植在清代逐渐发展成为大田作物,越来越多的田地成为专种棉花的棉地、花地。与此相应,棉花的省内外贸易更加引人注目。天门岳口熊氏商家具体、详实的棉花经营记录表明,不同规模的花庄、花行以及个体商贩,活跃在乡村集市和不同层级的市镇。他们交易的棉花是本地产品,但并非自己的生产品,而是来自市场交换的商品。棉花不仅是“鄂民生计之一大宗”,亦构成区域社会经济发展不可或缺的重要方面。棉花产销两旺的兴盛景象,也为进一步深入思考诸如长距离贸易的性质、市场激励原理及明清农民经济行为等相关问题提供了更大的空间。