Objective: To explore the effects and mechanism of glycogen synthase kinase 3β (GSK-313) inhibitor (2'Z,3'E)-6-bromo-indirubin-3'-oxime (BIO) on drug resistance in colon cancer cells. Methods: The colon c...Objective: To explore the effects and mechanism of glycogen synthase kinase 3β (GSK-313) inhibitor (2'Z,3'E)-6-bromo-indirubin-3'-oxime (BIO) on drug resistance in colon cancer cells. Methods: The colon cancer SW480 and SW620 cells were treated with BIO, 5-fluorouracil (5-FU) and BIO/5-FU, separately. Cell cycle distribution, apoptosis level and efflux ability of rhodamine 123 (Rh123) were detected by flow cytometry. The protein expressions of P-glycoprotein (P-gp), multidrug resistance protein 2 (MRP2), thymidylate synthase (TS), β-catenin, E2F-1 and βcl-2 were detected by Western blot. β-catenin and P-gp were stained with double immunofluorescence and observed under a confocal microscope. Results: BIO up-regulated β-catenin, P-gp, MRP2 and TS, enhanced the efflux ability of Rh123, decreased Bcl-2 protein and gave the opposite effect to E2F-1 protein in SW480 and SW620 ceils. Furthermore, BIO significantly inhibited cell apoptosis, increased S and G2/M phase cells, and reduced the cell apoptosis induced by 5-FU in SW480 cells, whereas the effects were slight or not obvious in SW620 cells. Conclusion: GSK-3β was involved in drug resistance regulation, and activation of β-catenin and inhibition of E2F-1 may be the most responsible for the enhancement of 5-FU chemotherapy resistance induced by GSK-β inhibitor β10 in colon cancer.展开更多
With the widespread adoption of ultrasound guidance,Seldinger puncture techniques,and intracardiac electrical positioning technology for the placement of peripherally inserted central catheters in recent years,an incr...With the widespread adoption of ultrasound guidance,Seldinger puncture techniques,and intracardiac electrical positioning technology for the placement of peripherally inserted central catheters in recent years,an increasing number of medical staff and patients now accept peripheral placement of totally implantable venous access devices(TIVADs)in the upper arm.This approach has the advantage of completely avoiding the risks of hemothorax,pneumothorax,and neck and chest scarring.Medical specialties presently engaged in this study in China include internal medicine,surgery,anesthesiology,and interventional departments.However,command over implantation techniques,treatment of complications,and proper use and maintenance of TIVAD remain uneven among different medical units.Moreover,currently,there are no established quality control standards for implantation techniques or specifications for handling complications.Thus,this expert consensus is proposed to improve the success rate of TIVAD implantation via the upper-arm approach,reduce complication rates,and ensure patient safety.This consensus elaborates on the technical indications and contraindications,procedures and technical points,treatment of complications,and the use and maintenance of upper-arm TIVAD,thus providing a practical reference for medical staff.展开更多
Cu-based electrocatalysts have become the focus in the field of electrochemical CO_(2) reduction reaction(ECO_(2) RR)due to their ability to produce multicarbon products.However,the research on generating single carbo...Cu-based electrocatalysts have become the focus in the field of electrochemical CO_(2) reduction reaction(ECO_(2) RR)due to their ability to produce multicarbon products.However,the research on generating single carbon products with higher economic feasibility via ECO_(2) RR based on Cu-based electrocatalysts is rather rare,and the roles of the surface architecture and oxides of the electrocatalysts have not been explained exactly.In this work,a two-step method including thermal oxidation and electroreduction is proposed to introduce Cuþinto pure Cu foil to form Cu_(2)O/Cu electrocatalyst.By regulating the surface composition and morphology of the electrocatalyst in this way,the activity of ECO 2 RR to C_(1) products has been greatly improved.The Faradaic efficiency of carbon products of the Cu_(2)O/Cu electrode reaches 84%at?0.7 V vs.RHE with good selectivity for HCOOH and CO.The current density of Cu_(2)O/Cu electrode reaches-12.21 mA cm^(2) at-0.8 V vs.RHE,which is much higher than that of the Cu foil electrode(?0.09 mA cm?2).In-situ Raman characterization shows that Cuþin Cu_(2)O/Cu electrode could inhibit hydrogen generation and promote ECO_(2) RR by stabilizing the adsorption of CO_(2).展开更多
The underwater topography in the offshore sea area of north Jiangsu is complicated, including the middle radial sand ridges and northern Haizhou bay underwater shoal. Therefore, it forms special marine dynamic conditi...The underwater topography in the offshore sea area of north Jiangsu is complicated, including the middle radial sand ridges and northern Haizhou bay underwater shoal. Therefore, it forms special marine dynamic conditions and typical shoal wetland ecosystem. Previous researches of these sea areas were mainly focused on the forms of morphogenesis and the characteristic of conformation of sand ridges. Few studies have done on three dimensional hydrodynamic and water quality simulation. This paper introduced EFDC (Environ-mental Fluid Dynamics Code) to study the tidal current fields, dilution and diffusion of the sewage outlet near Yangkou Port. Comparison between computation results and the observed data indicates that this model could reasonably simulate hydrodynamic fields. Based on the computed tidal current field, the distributions of COD concentration were simulated. The range of contamination diffusion derived from sewage outlet was very limited, and the influence range of sewage came to the maximum when ebb slacks in neap tide period.展开更多
Postharvest broccoli is prone to yellowing during storage,which is the key factor leading to a reduction in value.To explore appropriate control methods,it is important to understand the mechanisms of yellowing.We ana...Postharvest broccoli is prone to yellowing during storage,which is the key factor leading to a reduction in value.To explore appropriate control methods,it is important to understand the mechanisms of yellowing.We analyzed the genes related to the metabolism of chlorophyll,carotenoids,and flavonoids and the transcription factors(TFs)involved in broccoli yellowing using transcriptome sequencing profiling.Broccoli stored at 10℃showed slight yellowing on postharvest day 5 and serious symptoms on day 12.There were significant changes in chlorophyll fluorescence kinetics,mainly manifesting as a decrease in the Fv/Fm value and an increase in nonphotochemical quenching,during the yellowing process.Transcriptome sequencing profiles from samples of fresh broccoli and broccoli with slight and severe yellowing revealed 6,5,and 4 differentially expressed genes involved in chlorophyll metabolism,carotenoid biosynthesis,and flavonoid biosynthesis,respectively.The transcription factor gene ontology categories showed that the MYB,bHLH,and bZip gene families were involved in chlorophyll metabolism.In addition,the transcription factor families included NACs and ethylene response factors(ERFs)that regulated carotenoid biosynthesis.Reverse transcription polymerase chain reaction further confirmed that bHLH66,PIF4,LOB13,NAC92,and APL were vital transcription factors that potentially regulated the CAO and HYD genes and were involved in chlorophyll metabolism and the carotenoid biosynthetic process.The flavonoid biosynthetic pathway was mainly regulated by MYBs,NACs,WRKYs,MADSs,and bZips.The results of the differentially expressed gene(DEG)and pigment content analyses indicated that the transcriptome data were accurately and positively associated with broccoli yellowing.展开更多
Objective:The aim of our research was to study the incidence,clinical diagnosis and treatment of peripherally inserted central catheters(PICC)-related sepsis in breast cancer patients carrying PICC catheter for chemot...Objective:The aim of our research was to study the incidence,clinical diagnosis and treatment of peripherally inserted central catheters(PICC)-related sepsis in breast cancer patients carrying PICC catheter for chemotherapy.Methods:The data of the incidence,diagnosis and treatment of PICC-related sepsis in 215 cases of breast cancer patients carrying PICC catheter for chemotherapy in our hospital from August,2009 to September,2011 were analyzed retrospectively.Results:216 PICCs had been successfully applied in 215 cases of breast cancer patients and followed for a total of 19,109 catheter days,(median catheterization duration,88.9 days,range 1-212 days).Among those,3(1.39%) PICCs were removed respectively as a result of PICC-related sepsis in 29,73 and 108 catheter-days(median 70 d),with a rate of 0.16 per 1000 catheter-days.Conclusion:Chemotherapeutic treatment via PICC for breast cancer patients is one of the most secure and effective measures but there exists small number of cases in which PICC catheter related sepsis is possible.Careful observation of relevant symptoms and signs,early diagnosis and treatment,not relying completely on blood culture tests and timely removal of PICC catheter can be very effective in treating PICC-related sepsis in these patients.展开更多
Huanglongbing(HLB),a systemic and destructive disease of citrus,is associated with‘Candidatus Liberibacter asiaticus’(Las)in the United States.Our earlier work has shown that Las bacteria were significantly reduced ...Huanglongbing(HLB),a systemic and destructive disease of citrus,is associated with‘Candidatus Liberibacter asiaticus’(Las)in the United States.Our earlier work has shown that Las bacteria were significantly reduced or eliminated when potted HLB-affected citrus were continuously exposed to high temperatures of 40 to 42°C for a minimum of 48 h.To determine the feasibility and effectiveness of solar thermotherapy in the field,portable plastic enclosures were placed over commercial and residential citrus,exposing trees to high temperatures through solarization.Within 3–6 weeks after treatment,most trees responded with vigorous new growth.Las titer in new growth was greatly reduced for 18–36 months after treatment.Unlike with potted trees,exposure to high heat did not eradicate the Las population under field conditions.This may be attributed to reduced temperatures at night in the field compared to continuous high temperature exposure that can be maintained in growth chambers,and the failure to achieve therapeutic temperatures in the root zone.Despite the presence of Las in heat-treated commercial citrus,many trees produced abundant flush and grew vigorously for 2 to 3 years after treatment.Transcriptome analysis comparing healthy trees to HLB-affected citrus both before and after heat treatment demonstrated that post-treatment transcriptional expression patterns more closely resembled the expression patterns of healthy controls for most differentially expressed genes and that genes involved with plant-bacterium interactions are upregulated after heat treatment.Overall,these results indicate that solar thermotherapy can be an effective component of an integrated control strategy for citrus HLB.展开更多
Hydrogen production by electrocatalytic water splitting promises a green and sustainable technology to address serious energy crisis and environmental pollution [1]. As well known, the process of electrocatalytic wate...Hydrogen production by electrocatalytic water splitting promises a green and sustainable technology to address serious energy crisis and environmental pollution [1]. As well known, the process of electrocatalytic water splitting is composed of two half reaction, i.e. oxygen evolution reaction (OER) at the anode and hydrogen evolution reaction (HER) at the cathode [2].展开更多
Massive binaries play significant roles in many fields.Identifying massive stars,particularly massive binaries,is of great importance.In this paper,by adopting the technique of measuring the equivalent widths of sever...Massive binaries play significant roles in many fields.Identifying massive stars,particularly massive binaries,is of great importance.In this paper,by adopting the technique of measuring the equivalent widths of several spectral lines,we identified 9382 early-type stars from the LAMOST medium-resolution survey and divided the sample into four groups,T1(~O-B4),T2(~B5),T3(~B7)and T4(~B8-A).The relative radial velocities RVrelwere calculated using Maximum Likelihood Estimation.The stars with significant changes of RVreland at least larger than 15.57 km s-1 were identified as spectroscopic binaries.We found that the observed spectroscopic binary fractions for the four groups are 24.6%±0.5%,20.8%±0.6%,13.7%±0.3%and 7.4%±0.3%,respectively.Assuming that orbital period(P)and mass ratio(q)have intrinsic distributions as f(P)∝Pπ(1<P<1000 days)and f(q)∝qκ(0.1<q<1),respectively,we conducted a series of Monte-Carlo simulations to correct observational biases for estimating the intrinsic multiplicity properties.The results show that the intrinsic binary fractions for the four groups are 68%±8%,52%±3%,44%±6%and 44%±6%,respectively.The best estimated values forπare-1±0.1,-1.1±0.05,-1.1±0.1 and-0.6±0.05,respectively.Theκcannot be constrained for groups T1 and T2 and is-2.4±0.3 for group T3 and-1.6±0.3 for group T4.We confirmed the relationship of a decreasing trend in binary fractions toward late-type stars.No correlation between the spectral type and orbital period distribution has been found yet,possibly due to the limitation of observational cadence.展开更多
Abstract:Binary stars play an important role in the evolution of stellar populations.The intrinsic binary fraction(fbin)of O and B-type(OB)stars in LAMOST DR5 was investigated in this work.We employed a cross-correlat...Abstract:Binary stars play an important role in the evolution of stellar populations.The intrinsic binary fraction(fbin)of O and B-type(OB)stars in LAMOST DR5 was investigated in this work.We employed a cross-correlation approach to estimate relative radial velocities for each of the stellar spectra.The algorithm described by Sana et al.(2013)was implemented and several simulations were made to assess the performance of the approach.The binary fraction of the OB stars is estimated through comparing the unidistribution between observations and simulations with the Kolmogorov-Smirnov tests.Simulations show that it is reliable for stars most of whom have six,seven and eight repeated observations.The uncertainty of orbital parameters of binarity becomes larger when observational frequencies decrease.By adopting the fixed power exponents ofπ=-0.45 andκ=-1 for period and mass ratio distributions,respectively,we obtain that fbin=0.4-0.06+0.05 for the samples with more than three observations.When we consider the full samples with at least two observations,the binary fraction turns out to be 0.37-0.03+0.03.These two results are consistent with each other in 1σ.展开更多
To carefully implement the rural revitalization strategy,further promote the structural reform of the agricultural supply front,implement the "155 Development Strategy",speed up the cultivation of the modern...To carefully implement the rural revitalization strategy,further promote the structural reform of the agricultural supply front,implement the "155 Development Strategy",speed up the cultivation of the modern agricultural industrial clusters with output of 100 billion yuan,and constantly consolidate the industrial foundation of the " Second City in Chengdu-Chongqing Line",the Municipal Party Committee and the Municipal Government of Nanchong City decided to implement the " 300 Demonstration Projects" of modern agriculture. It firmly grasps the " bull nose"( key part) of the project,energetically implements the construction of 100 modern circular agricultural demonstration parks,100 agricultural leading enterprises with output value of 100 billion yuan,and 100 agricultural new business model demonstration projects( hereinafter referred to as 300 Demonstration Projects),to realize the goal of the output value of the modern agriculture industrial clusters of Nanchong City exceeds 100 billion yuan in 2020.展开更多
Objective:To investigate the effect of single-operation-hole thoracoscopic surgery on elderly solitary pulmonary nodules and its effects on lung function and prognosis.Methods:64 patients with solitary pulmonary nodul...Objective:To investigate the effect of single-operation-hole thoracoscopic surgery on elderly solitary pulmonary nodules and its effects on lung function and prognosis.Methods:64 patients with solitary pulmonary nodules admitted to our hospital were selected. According to the different surgical treatment methods, they were divided into the study group and the control group, each group with 32 cases. The study group was treated with single-operation-hole thoracoscopic pulmonary nodule resection, and the control group was treated with conventional multi-operation-holes thoracoscopic pulmonary nodule resection. The clinical efficacy of the two groups was compared. Results: The postoperative drainage time, intraoperative blood loss, hospitalization time, postoperative drainage volume and hospitalization cost of the study group were significantly better than those of the control group (P<0.05), and there were no significant differences between the two groups in terms of the duration of operation and the incidence of adverse reactions (such as postoperative pulmonary atelectasis and pulmonary infection) (P>0.05). There was no difference in lung function between the two groups before operation (P>0.05). Compared with the control group, the lung function indexes of the postoperative study group were significantly improved (P<0.05). There was no difference in the overall survival rate and tumor-free survival rate of the two groups after operation for half a year (P>0.05). The VAS scores of the study group were significantly lower than those of the control group on the 1st and 3rd day after operation (P<0.05). There was no difference in the quality of life between the two groups before treatment (P>0.05), but the overall health status, physiological feature, physiological function, physical pain and energy of the patients in the study group after treatment were significantly higher than those in the control group (P<0.05), but there was no significant difference in social function, emotional function and mental health (P>0.05).Conclusions: Single-operation-hole thoracoscopic surgery is effective in the treatment of elderly solitary pulmonary nodules. It can not only accelerate the recovery of patients but also improve their lung function, without affecting the prognosis of patients, which can improve their quality of life. It is worthy of clinical application, but should strictly control the indications for surgery.展开更多
Fluorinated diols(FDO)with dangling chain were introduced into polyether urethanes(PEUs)as chain extender in order to achieve internal plasticization.Based on temperature dependent Fourier transform infrared(FTIR)spec...Fluorinated diols(FDO)with dangling chain were introduced into polyether urethanes(PEUs)as chain extender in order to achieve internal plasticization.Based on temperature dependent Fourier transform infrared(FTIR)spectroscopy results,the fluorinated polyether urethanes(FPEUs)exhibited weaker hydrogen bonding between C=O and N―H groups with addition of FDO.The crystallinity of hard domain was destroyed determined by thermal analysis.Combined with the results of dynamic mechanical analysis(DMA)and dissipative particle dynamics(DPD)simulation,it was proved that more loosely aggregated hard domains with weakened interaction separated from soft segment were formed.Rheological analysis indicated that FDO reduced the complex viscosity and viscous flow activation energy which endowed the polymer with more flexibility.Despite the loss of hydrogen bonds,there was no significant decline in mechanical property resulting from the decrease of hard segment dissolved in soft segment phase.The presence of fluorinated carbon side chain could function as internal plasticizer and contribute to the processability of polyurethane.展开更多
基金supported by the Sci-Tech Project Foundation of Guangdong Province(No.2009B080800023)
文摘Objective: To explore the effects and mechanism of glycogen synthase kinase 3β (GSK-313) inhibitor (2'Z,3'E)-6-bromo-indirubin-3'-oxime (BIO) on drug resistance in colon cancer cells. Methods: The colon cancer SW480 and SW620 cells were treated with BIO, 5-fluorouracil (5-FU) and BIO/5-FU, separately. Cell cycle distribution, apoptosis level and efflux ability of rhodamine 123 (Rh123) were detected by flow cytometry. The protein expressions of P-glycoprotein (P-gp), multidrug resistance protein 2 (MRP2), thymidylate synthase (TS), β-catenin, E2F-1 and βcl-2 were detected by Western blot. β-catenin and P-gp were stained with double immunofluorescence and observed under a confocal microscope. Results: BIO up-regulated β-catenin, P-gp, MRP2 and TS, enhanced the efflux ability of Rh123, decreased Bcl-2 protein and gave the opposite effect to E2F-1 protein in SW480 and SW620 ceils. Furthermore, BIO significantly inhibited cell apoptosis, increased S and G2/M phase cells, and reduced the cell apoptosis induced by 5-FU in SW480 cells, whereas the effects were slight or not obvious in SW620 cells. Conclusion: GSK-3β was involved in drug resistance regulation, and activation of β-catenin and inhibition of E2F-1 may be the most responsible for the enhancement of 5-FU chemotherapy resistance induced by GSK-β inhibitor β10 in colon cancer.
文摘With the widespread adoption of ultrasound guidance,Seldinger puncture techniques,and intracardiac electrical positioning technology for the placement of peripherally inserted central catheters in recent years,an increasing number of medical staff and patients now accept peripheral placement of totally implantable venous access devices(TIVADs)in the upper arm.This approach has the advantage of completely avoiding the risks of hemothorax,pneumothorax,and neck and chest scarring.Medical specialties presently engaged in this study in China include internal medicine,surgery,anesthesiology,and interventional departments.However,command over implantation techniques,treatment of complications,and proper use and maintenance of TIVAD remain uneven among different medical units.Moreover,currently,there are no established quality control standards for implantation techniques or specifications for handling complications.Thus,this expert consensus is proposed to improve the success rate of TIVAD implantation via the upper-arm approach,reduce complication rates,and ensure patient safety.This consensus elaborates on the technical indications and contraindications,procedures and technical points,treatment of complications,and the use and maintenance of upper-arm TIVAD,thus providing a practical reference for medical staff.
基金supported by National Natural Science Foundation of China(No.52071183).
文摘Cu-based electrocatalysts have become the focus in the field of electrochemical CO_(2) reduction reaction(ECO_(2) RR)due to their ability to produce multicarbon products.However,the research on generating single carbon products with higher economic feasibility via ECO_(2) RR based on Cu-based electrocatalysts is rather rare,and the roles of the surface architecture and oxides of the electrocatalysts have not been explained exactly.In this work,a two-step method including thermal oxidation and electroreduction is proposed to introduce Cuþinto pure Cu foil to form Cu_(2)O/Cu electrocatalyst.By regulating the surface composition and morphology of the electrocatalyst in this way,the activity of ECO 2 RR to C_(1) products has been greatly improved.The Faradaic efficiency of carbon products of the Cu_(2)O/Cu electrode reaches 84%at?0.7 V vs.RHE with good selectivity for HCOOH and CO.The current density of Cu_(2)O/Cu electrode reaches-12.21 mA cm^(2) at-0.8 V vs.RHE,which is much higher than that of the Cu foil electrode(?0.09 mA cm?2).In-situ Raman characterization shows that Cuþin Cu_(2)O/Cu electrode could inhibit hydrogen generation and promote ECO_(2) RR by stabilizing the adsorption of CO_(2).
文摘The underwater topography in the offshore sea area of north Jiangsu is complicated, including the middle radial sand ridges and northern Haizhou bay underwater shoal. Therefore, it forms special marine dynamic conditions and typical shoal wetland ecosystem. Previous researches of these sea areas were mainly focused on the forms of morphogenesis and the characteristic of conformation of sand ridges. Few studies have done on three dimensional hydrodynamic and water quality simulation. This paper introduced EFDC (Environ-mental Fluid Dynamics Code) to study the tidal current fields, dilution and diffusion of the sewage outlet near Yangkou Port. Comparison between computation results and the observed data indicates that this model could reasonably simulate hydrodynamic fields. Based on the computed tidal current field, the distributions of COD concentration were simulated. The range of contamination diffusion derived from sewage outlet was very limited, and the influence range of sewage came to the maximum when ebb slacks in neap tide period.
基金supported by the National Key R&D Program of China(no.2016YFD0400103).
文摘Postharvest broccoli is prone to yellowing during storage,which is the key factor leading to a reduction in value.To explore appropriate control methods,it is important to understand the mechanisms of yellowing.We analyzed the genes related to the metabolism of chlorophyll,carotenoids,and flavonoids and the transcription factors(TFs)involved in broccoli yellowing using transcriptome sequencing profiling.Broccoli stored at 10℃showed slight yellowing on postharvest day 5 and serious symptoms on day 12.There were significant changes in chlorophyll fluorescence kinetics,mainly manifesting as a decrease in the Fv/Fm value and an increase in nonphotochemical quenching,during the yellowing process.Transcriptome sequencing profiles from samples of fresh broccoli and broccoli with slight and severe yellowing revealed 6,5,and 4 differentially expressed genes involved in chlorophyll metabolism,carotenoid biosynthesis,and flavonoid biosynthesis,respectively.The transcription factor gene ontology categories showed that the MYB,bHLH,and bZip gene families were involved in chlorophyll metabolism.In addition,the transcription factor families included NACs and ethylene response factors(ERFs)that regulated carotenoid biosynthesis.Reverse transcription polymerase chain reaction further confirmed that bHLH66,PIF4,LOB13,NAC92,and APL were vital transcription factors that potentially regulated the CAO and HYD genes and were involved in chlorophyll metabolism and the carotenoid biosynthetic process.The flavonoid biosynthetic pathway was mainly regulated by MYBs,NACs,WRKYs,MADSs,and bZips.The results of the differentially expressed gene(DEG)and pigment content analyses indicated that the transcriptome data were accurately and positively associated with broccoli yellowing.
文摘Objective:The aim of our research was to study the incidence,clinical diagnosis and treatment of peripherally inserted central catheters(PICC)-related sepsis in breast cancer patients carrying PICC catheter for chemotherapy.Methods:The data of the incidence,diagnosis and treatment of PICC-related sepsis in 215 cases of breast cancer patients carrying PICC catheter for chemotherapy in our hospital from August,2009 to September,2011 were analyzed retrospectively.Results:216 PICCs had been successfully applied in 215 cases of breast cancer patients and followed for a total of 19,109 catheter days,(median catheterization duration,88.9 days,range 1-212 days).Among those,3(1.39%) PICCs were removed respectively as a result of PICC-related sepsis in 29,73 and 108 catheter-days(median 70 d),with a rate of 0.16 per 1000 catheter-days.Conclusion:Chemotherapeutic treatment via PICC for breast cancer patients is one of the most secure and effective measures but there exists small number of cases in which PICC catheter related sepsis is possible.Careful observation of relevant symptoms and signs,early diagnosis and treatment,not relying completely on blood culture tests and timely removal of PICC catheter can be very effective in treating PICC-related sepsis in these patients.
基金This work was funded by the Florida Department of Agriculture and Consumer Services Specialty Crop Block Grant#018023 and the Citrus Research and Development Foundation#834.
文摘Huanglongbing(HLB),a systemic and destructive disease of citrus,is associated with‘Candidatus Liberibacter asiaticus’(Las)in the United States.Our earlier work has shown that Las bacteria were significantly reduced or eliminated when potted HLB-affected citrus were continuously exposed to high temperatures of 40 to 42°C for a minimum of 48 h.To determine the feasibility and effectiveness of solar thermotherapy in the field,portable plastic enclosures were placed over commercial and residential citrus,exposing trees to high temperatures through solarization.Within 3–6 weeks after treatment,most trees responded with vigorous new growth.Las titer in new growth was greatly reduced for 18–36 months after treatment.Unlike with potted trees,exposure to high heat did not eradicate the Las population under field conditions.This may be attributed to reduced temperatures at night in the field compared to continuous high temperature exposure that can be maintained in growth chambers,and the failure to achieve therapeutic temperatures in the root zone.Despite the presence of Las in heat-treated commercial citrus,many trees produced abundant flush and grew vigorously for 2 to 3 years after treatment.Transcriptome analysis comparing healthy trees to HLB-affected citrus both before and after heat treatment demonstrated that post-treatment transcriptional expression patterns more closely resembled the expression patterns of healthy controls for most differentially expressed genes and that genes involved with plant-bacterium interactions are upregulated after heat treatment.Overall,these results indicate that solar thermotherapy can be an effective component of an integrated control strategy for citrus HLB.
基金financially supported by the Natural Science Foundation of Jiangxi Province of China (20192BAB213001)the Foundation of Jiangxi Educational Committee (GJJ180406)+2 种基金the Research Foundation for Advanced Talents of East China University of Technology (DHBK2018043)the National Natural Science Foundation of China (2187104721661001)。
文摘Hydrogen production by electrocatalytic water splitting promises a green and sustainable technology to address serious energy crisis and environmental pollution [1]. As well known, the process of electrocatalytic water splitting is composed of two half reaction, i.e. oxygen evolution reaction (OER) at the anode and hydrogen evolution reaction (HER) at the cathode [2].
基金supported by the Natural Science Foundation of China(NSFC,Grant Nos.11733008,12090040,12090043,11521303,12125303)Yunnan Province and the National Tenthousand Talents Program+6 种基金National Key R&D Program of China No.2019YFA0405500the NSFC with Grant No.11835057The Guoshoujing Telescope(the Large Sky Area MultiObject Fiber Spectroscopic Telescope,LAMOST)is a National Major Scientific Project built by the Chinese Academy of SciencesFunding for the project has been provided by the National Development and Reform CommissionLAMOST is operated and managed by the National Astronomical Observatories,Chinese Academy of Sciencessupported by the Key Research Program of Frontier Sciences,CAS,Grant No.QYZDY-SSW-SLH007the science research grants from the China Manned Space Project with No.CMS-CSST-2021-A10。
文摘Massive binaries play significant roles in many fields.Identifying massive stars,particularly massive binaries,is of great importance.In this paper,by adopting the technique of measuring the equivalent widths of several spectral lines,we identified 9382 early-type stars from the LAMOST medium-resolution survey and divided the sample into four groups,T1(~O-B4),T2(~B5),T3(~B7)and T4(~B8-A).The relative radial velocities RVrelwere calculated using Maximum Likelihood Estimation.The stars with significant changes of RVreland at least larger than 15.57 km s-1 were identified as spectroscopic binaries.We found that the observed spectroscopic binary fractions for the four groups are 24.6%±0.5%,20.8%±0.6%,13.7%±0.3%and 7.4%±0.3%,respectively.Assuming that orbital period(P)and mass ratio(q)have intrinsic distributions as f(P)∝Pπ(1<P<1000 days)and f(q)∝qκ(0.1<q<1),respectively,we conducted a series of Monte-Carlo simulations to correct observational biases for estimating the intrinsic multiplicity properties.The results show that the intrinsic binary fractions for the four groups are 68%±8%,52%±3%,44%±6%and 44%±6%,respectively.The best estimated values forπare-1±0.1,-1.1±0.05,-1.1±0.1 and-0.6±0.05,respectively.Theκcannot be constrained for groups T1 and T2 and is-2.4±0.3 for group T3 and-1.6±0.3 for group T4.We confirmed the relationship of a decreasing trend in binary fractions toward late-type stars.No correlation between the spectral type and orbital period distribution has been found yet,possibly due to the limitation of observational cadence.
基金the National Key R&D Program of China(No.2019 YFA0405501)supported by the National Natural Science Foundation of China(Grant Nos.11835057,12090040 and 12090043)Guo Shou Jing Telescope(the Large Sky Area Multi-Object Fiber Spectroscopic Telescope LAMOST)is a National Major Scientific Project built by the Chinese Academy of Sciences.Funding for the project has been provided by the National Development and Reform Commission。
文摘Abstract:Binary stars play an important role in the evolution of stellar populations.The intrinsic binary fraction(fbin)of O and B-type(OB)stars in LAMOST DR5 was investigated in this work.We employed a cross-correlation approach to estimate relative radial velocities for each of the stellar spectra.The algorithm described by Sana et al.(2013)was implemented and several simulations were made to assess the performance of the approach.The binary fraction of the OB stars is estimated through comparing the unidistribution between observations and simulations with the Kolmogorov-Smirnov tests.Simulations show that it is reliable for stars most of whom have six,seven and eight repeated observations.The uncertainty of orbital parameters of binarity becomes larger when observational frequencies decrease.By adopting the fixed power exponents ofπ=-0.45 andκ=-1 for period and mass ratio distributions,respectively,we obtain that fbin=0.4-0.06+0.05 for the samples with more than three observations.When we consider the full samples with at least two observations,the binary fraction turns out to be 0.37-0.03+0.03.These two results are consistent with each other in 1σ.
基金the Project of National Modern Agriculture Demonstration Area of the Ministry of Agriculture(Nong Ji Fa[2010]22)Project of Nanchong City National Modern Agriculture Demonstration Area Agricultural Reform and Construction Pilot Demonstration Area of the Ministry of Agriculture and Ministry of Finance(Nong Cai Fa[2013]13)Project of Nanchong City Nanchong National Agricultural Science and Technology Park of Ministry of Science and Technology(Guo Ke Ban Nong[2015]9)
文摘To carefully implement the rural revitalization strategy,further promote the structural reform of the agricultural supply front,implement the "155 Development Strategy",speed up the cultivation of the modern agricultural industrial clusters with output of 100 billion yuan,and constantly consolidate the industrial foundation of the " Second City in Chengdu-Chongqing Line",the Municipal Party Committee and the Municipal Government of Nanchong City decided to implement the " 300 Demonstration Projects" of modern agriculture. It firmly grasps the " bull nose"( key part) of the project,energetically implements the construction of 100 modern circular agricultural demonstration parks,100 agricultural leading enterprises with output value of 100 billion yuan,and 100 agricultural new business model demonstration projects( hereinafter referred to as 300 Demonstration Projects),to realize the goal of the output value of the modern agriculture industrial clusters of Nanchong City exceeds 100 billion yuan in 2020.
文摘Objective:To investigate the effect of single-operation-hole thoracoscopic surgery on elderly solitary pulmonary nodules and its effects on lung function and prognosis.Methods:64 patients with solitary pulmonary nodules admitted to our hospital were selected. According to the different surgical treatment methods, they were divided into the study group and the control group, each group with 32 cases. The study group was treated with single-operation-hole thoracoscopic pulmonary nodule resection, and the control group was treated with conventional multi-operation-holes thoracoscopic pulmonary nodule resection. The clinical efficacy of the two groups was compared. Results: The postoperative drainage time, intraoperative blood loss, hospitalization time, postoperative drainage volume and hospitalization cost of the study group were significantly better than those of the control group (P<0.05), and there were no significant differences between the two groups in terms of the duration of operation and the incidence of adverse reactions (such as postoperative pulmonary atelectasis and pulmonary infection) (P>0.05). There was no difference in lung function between the two groups before operation (P>0.05). Compared with the control group, the lung function indexes of the postoperative study group were significantly improved (P<0.05). There was no difference in the overall survival rate and tumor-free survival rate of the two groups after operation for half a year (P>0.05). The VAS scores of the study group were significantly lower than those of the control group on the 1st and 3rd day after operation (P<0.05). There was no difference in the quality of life between the two groups before treatment (P>0.05), but the overall health status, physiological feature, physiological function, physical pain and energy of the patients in the study group after treatment were significantly higher than those in the control group (P<0.05), but there was no significant difference in social function, emotional function and mental health (P>0.05).Conclusions: Single-operation-hole thoracoscopic surgery is effective in the treatment of elderly solitary pulmonary nodules. It can not only accelerate the recovery of patients but also improve their lung function, without affecting the prognosis of patients, which can improve their quality of life. It is worthy of clinical application, but should strictly control the indications for surgery.
基金financially supported by the National Natural Science Foundation of China(Nos.51973134 and 51703139)the State Key Research Development Program of China(No.2020YFC1107000)+2 种基金Fund of Cooperation of Sichuan UniversityDazhou(No.2020CDDZ-14)Chengdu Scientific Program R&D Key Project(No.2019-YF05-00156-SN)the project of the Science and Technology Department in Sichuan Province(No.2020YJ0230)。
文摘Fluorinated diols(FDO)with dangling chain were introduced into polyether urethanes(PEUs)as chain extender in order to achieve internal plasticization.Based on temperature dependent Fourier transform infrared(FTIR)spectroscopy results,the fluorinated polyether urethanes(FPEUs)exhibited weaker hydrogen bonding between C=O and N―H groups with addition of FDO.The crystallinity of hard domain was destroyed determined by thermal analysis.Combined with the results of dynamic mechanical analysis(DMA)and dissipative particle dynamics(DPD)simulation,it was proved that more loosely aggregated hard domains with weakened interaction separated from soft segment were formed.Rheological analysis indicated that FDO reduced the complex viscosity and viscous flow activation energy which endowed the polymer with more flexibility.Despite the loss of hydrogen bonds,there was no significant decline in mechanical property resulting from the decrease of hard segment dissolved in soft segment phase.The presence of fluorinated carbon side chain could function as internal plasticizer and contribute to the processability of polyurethane.