Glycogen Synthase Kinase 3β Inhibitor (2'Z,3'E)-6-Bromo-indirubin-3'-Oxime Enhances Drug Resistance to 5-Fluorouracil Chemotherapy in Colon Cancer Cells

Objective： To explore the effects and mechanism of glycogen synthase kinase 3β （GSK-313） inhibitor （2＇Z,3＇E）-6-bromo-indirubin-3＇-oxime （BIO） on drug resistance in colon cancer cells. Methods： The colon cancer SW480 and SW620 cells were treated with BIO, 5-fluorouracil （5-FU） and BIO/5-FU, separately. Cell cycle distribution, apoptosis level and efflux ability of rhodamine 123 （Rh123） were detected by flow cytometry. The protein expressions of P-glycoprotein （P-gp）, multidrug resistance protein 2 （MRP2）, thymidylate synthase （TS）, β-catenin, E2F-1 and βcl-2 were detected by Western blot. β-catenin and P-gp were stained with double immunofluorescence and observed under a confocal microscope. Results： BIO up-regulated β-catenin, P-gp, MRP2 and TS, enhanced the efflux ability of Rh123, decreased Bcl-2 protein and gave the opposite effect to E2F-1 protein in SW480 and SW620 ceils. Furthermore, BIO significantly inhibited cell apoptosis, increased S and G2/M phase cells, and reduced the cell apoptosis induced by 5-FU in SW480 cells, whereas the effects were slight or not obvious in SW620 cells. Conclusion： GSK-3β was involved in drug resistance regulation, and activation of β-catenin and inhibition of E2F-1 may be the most responsible for the enhancement of 5-FU chemotherapy resistance induced by GSK-β inhibitor β10 in colon cancer.

Expert consensus on the clinical application of totally implantable venous access devices in the upper arm(2022 Edition)

With the widespread adoption of ultrasound guidance,Seldinger puncture techniques,and intracardiac electrical positioning technology for the placement of peripherally inserted central catheters in recent years,an increasing number of medical staff and patients now accept peripheral placement of totally implantable venous access devices(TIVADs)in the upper arm.This approach has the advantage of completely avoiding the risks of hemothorax,pneumothorax,and neck and chest scarring.Medical specialties presently engaged in this study in China include internal medicine,surgery,anesthesiology,and interventional departments.However,command over implantation techniques,treatment of complications,and proper use and maintenance of TIVAD remain uneven among different medical units.Moreover,currently,there are no established quality control standards for implantation techniques or specifications for handling complications.Thus,this expert consensus is proposed to improve the success rate of TIVAD implantation via the upper-arm approach,reduce complication rates,and ensure patient safety.This consensus elaborates on the technical indications and contraindications,procedures and technical points,treatment of complications,and the use and maintenance of upper-arm TIVAD,thus providing a practical reference for medical staff.

Improving the activity of electrochemical reduction of CO_(2) to C_(1) products by oxidation derived copper catalyst

Cu-based electrocatalysts have become the focus in the field of electrochemical CO_(2) reduction reaction(ECO_(2) RR)due to their ability to produce multicarbon products.However,the research on generating single carbon products with higher economic feasibility via ECO_(2) RR based on Cu-based electrocatalysts is rather rare,and the roles of the surface architecture and oxides of the electrocatalysts have not been explained exactly.In this work,a two-step method including thermal oxidation and electroreduction is proposed to introduce Cuþinto pure Cu foil to form Cu_(2)O/Cu electrocatalyst.By regulating the surface composition and morphology of the electrocatalyst in this way,the activity of ECO 2 RR to C_(1) products has been greatly improved.The Faradaic efficiency of carbon products of the Cu_(2)O/Cu electrode reaches 84%at?0.7 V vs.RHE with good selectivity for HCOOH and CO.The current density of Cu_(2)O/Cu electrode reaches-12.21 mA cm^(2) at-0.8 V vs.RHE,which is much higher than that of the Cu foil electrode(?0.09 mA cm?2).In-situ Raman characterization shows that Cuþin Cu_(2)O/Cu electrode could inhibit hydrogen generation and promote ECO_(2) RR by stabilizing the adsorption of CO_(2).

肺结节/肺癌患者全程管理模式的设计与应用

背景与目的早期筛查是有效降低肺癌死亡率的重要方式,然而我国尚缺乏统一、有效的肺癌早期筛查标准与手段。本研究基于四川大学华西医院构建的肺结节/肺癌患者全程管理平台,将肺结节早期筛查纳入肺癌规范诊疗体系,以期实现提升肺癌患者生存率。方法建立三大研究队列:健康人群筛查队列、肺结节患者队列、肺癌患者队列,系统、持续收集、分析各队列人群临床诊疗相关大数据。初步计划对肺结节早期筛查效果进行验证。结果该平台初步以2015年1月1日-2019年12月31日华西医院40岁以上职工2,836人作为实施对象,共诊断66例肺癌,均经外科手术切除确诊为早期肺癌65例,1例肺癌伴脑转移。结论肺结节/肺癌患者全程管理项目可实现肺结节高效筛查、随访与诊疗,未来全程管理模式将全面覆盖华西医疗系统,发挥区域带动作用。

扫频超声处理时间对β-乳球蛋白酶解制备多肽抗氧化活性的影响

目的:研究不同扫频超声处理时间对β-乳球蛋白酶解制备多肽抗氧化活性的影响。方法:研究不同的超声预处理时间(10,20,30,60,90 min)对β-乳球蛋白表观结构的影响,以及超声波处理对β-乳球蛋白酶解产物的抗氧化活性、氨基酸组成、分子质量分布和疏水性的影响。结论:超声波处理可显著提高β-乳球蛋白酶解产物的DPPH自由基清除率、ABTS·清除能力和Fe2+络合能力。随着超声时间的延长,β-乳球蛋白酶解产物抗氧化活性呈先增加后降低的趋势。扫频超声波处理可以提高β-LG酶解产物的疏水性,并且显著增加多肽中的疏水性氨基酸的含量。扫频超声处理10~60 min有利于分子质量为200~2000 u多肽的生成,从而提高其酶解产物的抗氧化活性。粒径分布表明短时间的超声处理(<30 min)引起β-LG粒径减小,大分子蛋白的结构疏松,分子间的疏水性作用力增加;长时间(60~90 min)的超声处理则引起大分子蛋白聚集,疏水性作用力降低,蛋白颗粒粒径增大。

新型次磷酸钠体系化学镀铜添加剂及其对镀液和镀层性能的影响

目的将聚乙烯吡咯烷酮和二苯胺磺酸钠作为添加剂应用到次磷酸钠化学镀铜体系,并获取最佳应用效果的工艺和条件。方法以PCB环氧树脂板为基材,通过电化学方法研究以聚乙烯吡咯烷酮和二苯胺磺酸钠为添加剂的次磷酸钠体系化学镀铜液及其性能,用称量法研究添加剂对沉积速率的影响,用扫描电镜和X射线能谱仪分析添加剂对沉铜表面质量的影响,用极化曲线法研究添加剂对沉铜表面孔隙率的影响,用交流阻抗法研究添加剂对沉铜表面耐蚀性的影响,同时测定镀液的稳定性。结果在p H=10、温度为65℃的基础液(成分为5 g/L五水硫酸铜、30 g/L次磷酸钠、16 g/L柠檬酸钠、30 g/L硼酸、1 g/L硫酸镍)中,单独加入聚乙烯吡咯烷酮或二苯胺磺酸钠,都能起到很好的沉铜效果。它们的最佳质量浓度分别为20~28mg/L和50~58mg/L。当聚乙烯吡咯烷酮和二苯胺磺酸钠在最佳范围内组合使用时,获得的镀液更稳定,镀层孔隙率低、耐蚀性好、表面均匀,表面铜的质量分数达到95.52%,镀层显粉红色,沉积速率在1.5~2.5μm/h范围内,符合PCB行业要求。结论最佳的化学镀铜液配方和条件为:5 g/L五水硫酸铜、30 g/L次磷酸钠、16 g/L柠檬酸钠、30 g/L硼酸、1 g/L硫酸镍、20~28 mg/L聚乙烯吡咯烷酮、50~58 mg/L二苯胺磺酸钠,pH=10,温度65℃。研究成果对电镀铜添加剂的开发和应用有重要意义。

聚二甲基硅氧烷-水性聚氨酯的合成与性能

为了提高水性聚氨酯的耐水性和耐候性,文中以羟烷基聚二甲基硅氧烷(PDMS)和聚己二酸丁二醇酯二醇(PBA)为混合软段,间苯二亚甲基二异氰酸酯(XDI)和1,4-丁二醇(BDO)、二羟甲基丙酸(DMPA)为硬段,采用二步本体法合成一系列的聚二甲基硅氧烷-水性聚氨酯(Si-XWPUs),研究了PDMS在软段中的含量对水性聚氨酯的表面性能、力学性能和耐水性的影响。研究发现,随着PDMS在软段中的含量从0%到20%逐渐增加,Si-XWPUs的微相分离程度略有提高,拉伸强度均达到10 MPa以上,作为涂层材料均具有较好的力学性能。同时,随PDMS在软段中比例的升高,Si-XWPUs薄膜表面Si元素的百分比呈增加趋势,并且表面的Si/C含量远远高于本体中理论Si/C含量,最高的迁移率达24倍,表现出良好的疏水性,同时吸水率降低。

小细胞肺癌合并腺癌MDT治疗报道及文献综述

小细胞肺癌(small cell lung cancer,SCLC)是肺癌中恶性程度和死亡率最高的类型。目前的一线标准治疗方式仍是以依托泊苷和铂类为主的化疗方案。然而,对于一线治疗后进展的SCLC,治疗方式仍非常有限。由于目前对SCLC一线耐药的分子机制尚未明了,而且一线耐药后的精准医疗策略仍处于临床前期阶段,因而在SCLC一线治疗进展后,进行二次活检及基因检测的比例很低。本文通过报道1例初次诊断为SCLC的中年女性,病程中多次活检发现肿瘤组织病理学类型出现具有敏感基因突变的腺癌以及小细胞癌的反复转变,提示其可能为SCLC中的特殊亚型——混合型小细胞肺癌(mixed small cell lung cancer,M-SCLC)。本例患者在治疗过程中先后使用了放化疗、免疫治疗及靶向治疗,目前生存时间已达2年8个月。本文通过病例报道及回顾性文献复习,旨在探讨对一部分可能一开始就呈现混合型病理组织类型或在治疗后出现组织类型改变的SCLC,疾病进展时有必要再次活检明确病理组织学类型及基因学检测,寻找潜在治疗靶点,以便给予基于分子标志物检测结果的精准治疗,为患者提供尽可能长的生存获益。

铜离子萃取剂的研究进展

概述了溶剂萃取过程中常用的铜离子萃取剂,主要包括肟类、β-二酮类、三元胺类和复配类,详细介绍了它们的性能、结构,以及在湿法冶金、含铜废弃物处理等方面的用途。

Serum tumor markers for detection of hepatocellular carcinoma

肝细胞癌(HCC ) 是最经常的恶性瘤之一并且是在中国的癌症死亡的第二个很普通的原因。因此，在它的更早的时期检测这疾病和复发是很重要的。作为为很长时间检测肝细胞癌的有效方法，浆液肿瘤标记能被划分成 4 个范畴：oncofetal 抗原和糖蛋白抗原；酶和 isoenzymes；基因；并且 cytokines。浆液高山哈 fetoprotein (法新社) 是在与肝细胞癌检测病人的最广泛地使用的肿瘤标记，并且被证明了有预示的能力预后。然而， AFP-L3 和 DCP 在把肝细胞癌区分开来与非恶意的肝病并且检测小肝细胞癌胜过法新社，这被显示了。一些肿瘤标记例如人的颈的癌症 oncogene 和人的 telomerase 颠倒 transcriptase mRNA，也被显示了比法新社有更高的精确性。而且，一些另外的肿瘤标记例如 glypican-3， gamma-glutamyl 转移酶 II， alpha-l-fucosidase，转变生长 factor-beta1，肿瘤特定的生长因素，被显示了是到在察觉的法新社的可得到的 supplementaries。法新社 mRNA 被显示了与 HCC 的转移和复发相关，并且预示可以是最有用的标记预后。一些另外的标记例如 gamma-glutamyl 转移酶 mRNA，脉管的内皮生长因素，和 interleukin-8，能也被用作可得到的预示的指示物，和法新社的同时的决心，这些标记可以在它的更早的时期检测 HCC 的复发。

3D Water Environment Simulation for North Jiangsu Offshore Sea Based on EFDC

The underwater topography in the offshore sea area of north Jiangsu is complicated, including the middle radial sand ridges and northern Haizhou bay underwater shoal. Therefore, it forms special marine dynamic conditions and typical shoal wetland ecosystem. Previous researches of these sea areas were mainly focused on the forms of morphogenesis and the characteristic of conformation of sand ridges. Few studies have done on three dimensional hydrodynamic and water quality simulation. This paper introduced EFDC (Environ-mental Fluid Dynamics Code) to study the tidal current fields, dilution and diffusion of the sewage outlet near Yangkou Port. Comparison between computation results and the observed data indicates that this model could reasonably simulate hydrodynamic fields. Based on the computed tidal current field, the distributions of COD concentration were simulated. The range of contamination diffusion derived from sewage outlet was very limited, and the influence range of sewage came to the maximum when ebb slacks in neap tide period.

Transcriptome profiling reveals the roles of pigment mechanisms in postharvest broccoli yellowing

Postharvest broccoli is prone to yellowing during storage,which is the key factor leading to a reduction in value.To explore appropriate control methods,it is important to understand the mechanisms of yellowing.We analyzed the genes related to the metabolism of chlorophyll,carotenoids,and flavonoids and the transcription factors(TFs)involved in broccoli yellowing using transcriptome sequencing profiling.Broccoli stored at 10℃showed slight yellowing on postharvest day 5 and serious symptoms on day 12.There were significant changes in chlorophyll fluorescence kinetics,mainly manifesting as a decrease in the Fv/Fm value and an increase in nonphotochemical quenching,during the yellowing process.Transcriptome sequencing profiles from samples of fresh broccoli and broccoli with slight and severe yellowing revealed 6,5,and 4 differentially expressed genes involved in chlorophyll metabolism,carotenoid biosynthesis,and flavonoid biosynthesis,respectively.The transcription factor gene ontology categories showed that the MYB,bHLH,and bZip gene families were involved in chlorophyll metabolism.In addition,the transcription factor families included NACs and ethylene response factors(ERFs)that regulated carotenoid biosynthesis.Reverse transcription polymerase chain reaction further confirmed that bHLH66,PIF4,LOB13,NAC92,and APL were vital transcription factors that potentially regulated the CAO and HYD genes and were involved in chlorophyll metabolism and the carotenoid biosynthetic process.The flavonoid biosynthetic pathway was mainly regulated by MYBs,NACs,WRKYs,MADSs,and bZips.The results of the differentially expressed gene(DEG)and pigment content analyses indicated that the transcriptome data were accurately and positively associated with broccoli yellowing.

乳腺癌患者携邛PICC化疗并发导管败血症的诊治探讨(英文)

Objective:The aim of our research was to study the incidence,clinical diagnosis and treatment of peripherally inserted central catheters(PICC)-related sepsis in breast cancer patients carrying PICC catheter for chemotherapy.Methods:The data of the incidence,diagnosis and treatment of PICC-related sepsis in 215 cases of breast cancer patients carrying PICC catheter for chemotherapy in our hospital from August,2009 to September,2011 were analyzed retrospectively.Results:216 PICCs had been successfully applied in 215 cases of breast cancer patients and followed for a total of 19,109 catheter days,(median catheterization duration,88.9 days,range 1-212 days).Among those,3(1.39%) PICCs were removed respectively as a result of PICC-related sepsis in 29,73 and 108 catheter-days(median 70 d),with a rate of 0.16 per 1000 catheter-days.Conclusion:Chemotherapeutic treatment via PICC for breast cancer patients is one of the most secure and effective measures but there exists small number of cases in which PICC catheter related sepsis is possible.Careful observation of relevant symptoms and signs,early diagnosis and treatment,not relying completely on blood culture tests and timely removal of PICC catheter can be very effective in treating PICC-related sepsis in these patients.

Solar thermotherapy reduces the titer of Candidatus Liberibacter asiaticus and enhances canopy growth by altering gene expression profiles in HLB-affected citrus plants

Huanglongbing(HLB),a systemic and destructive disease of citrus,is associated with‘Candidatus Liberibacter asiaticus’(Las)in the United States.Our earlier work has shown that Las bacteria were significantly reduced or eliminated when potted HLB-affected citrus were continuously exposed to high temperatures of 40 to 42°C for a minimum of 48 h.To determine the feasibility and effectiveness of solar thermotherapy in the field,portable plastic enclosures were placed over commercial and residential citrus,exposing trees to high temperatures through solarization.Within 3–6 weeks after treatment,most trees responded with vigorous new growth.Las titer in new growth was greatly reduced for 18–36 months after treatment.Unlike with potted trees,exposure to high heat did not eradicate the Las population under field conditions.This may be attributed to reduced temperatures at night in the field compared to continuous high temperature exposure that can be maintained in growth chambers,and the failure to achieve therapeutic temperatures in the root zone.Despite the presence of Las in heat-treated commercial citrus,many trees produced abundant flush and grew vigorously for 2 to 3 years after treatment.Transcriptome analysis comparing healthy trees to HLB-affected citrus both before and after heat treatment demonstrated that post-treatment transcriptional expression patterns more closely resembled the expression patterns of healthy controls for most differentially expressed genes and that genes involved with plant-bacterium interactions are upregulated after heat treatment.Overall,these results indicate that solar thermotherapy can be an effective component of an integrated control strategy for citrus HLB.

Carambola-like metal-organic frameworks for high-performance electrocatalytic oxygen evolution reaction

Hydrogen production by electrocatalytic water splitting promises a green and sustainable technology to address serious energy crisis and environmental pollution [1]. As well known, the process of electrocatalytic water splitting is composed of two half reaction, i.e. oxygen evolution reaction (OER) at the anode and hydrogen evolution reaction (HER) at the cathode [2].

The Binarity of Early-type Stars from LAMOST medium-resolution Spectroscopic Survey

Massive binaries play significant roles in many fields.Identifying massive stars,particularly massive binaries,is of great importance.In this paper,by adopting the technique of measuring the equivalent widths of several spectral lines,we identified 9382 early-type stars from the LAMOST medium-resolution survey and divided the sample into four groups,T1(~O-B4),T2(~B5),T3(~B7)and T4(~B8-A).The relative radial velocities RVrelwere calculated using Maximum Likelihood Estimation.The stars with significant changes of RVreland at least larger than 15.57 km s-1 were identified as spectroscopic binaries.We found that the observed spectroscopic binary fractions for the four groups are 24.6%±0.5%,20.8%±0.6%,13.7%±0.3%and 7.4%±0.3%,respectively.Assuming that orbital period(P)and mass ratio(q)have intrinsic distributions as f(P)∝Pπ(1<P<1000 days)and f(q)∝qκ(0.1<q<1),respectively,we conducted a series of Monte-Carlo simulations to correct observational biases for estimating the intrinsic multiplicity properties.The results show that the intrinsic binary fractions for the four groups are 68%±8%,52%±3%,44%±6%and 44%±6%,respectively.The best estimated values forπare-1±0.1,-1.1±0.05,-1.1±0.1 and-0.6±0.05,respectively.Theκcannot be constrained for groups T1 and T2 and is-2.4±0.3 for group T3 and-1.6±0.3 for group T4.We confirmed the relationship of a decreasing trend in binary fractions toward late-type stars.No correlation between the spectral type and orbital period distribution has been found yet,possibly due to the limitation of observational cadence.

Binary fraction of O and B-type stars from LAMOST data

Abstract:Binary stars play an important role in the evolution of stellar populations.The intrinsic binary fraction(fbin)of O and B-type(OB)stars in LAMOST DR5 was investigated in this work.We employed a cross-correlation approach to estimate relative radial velocities for each of the stellar spectra.The algorithm described by Sana et al.(2013)was implemented and several simulations were made to assess the performance of the approach.The binary fraction of the OB stars is estimated through comparing the unidistribution between observations and simulations with the Kolmogorov-Smirnov tests.Simulations show that it is reliable for stars most of whom have six,seven and eight repeated observations.The uncertainty of orbital parameters of binarity becomes larger when observational frequencies decrease.By adopting the fixed power exponents ofπ=-0.45 andκ=-1 for period and mass ratio distributions,respectively,we obtain that fbin=0.4-0.06+0.05 for the samples with more than three observations.When we consider the full samples with at least two observations,the binary fraction turns out to be 0.37-0.03+0.03.These two results are consistent with each other in 1σ.

Strategic Measures for Implementing 300 Demonstration Projects to Lead the Construction of Modern Agricultural Industrial Clusters with Output of 100 Billion Yuan

To carefully implement the rural revitalization strategy,further promote the structural reform of the agricultural supply front,implement the "155 Development Strategy",speed up the cultivation of the modern agricultural industrial clusters with output of 100 billion yuan,and constantly consolidate the industrial foundation of the " Second City in Chengdu-Chongqing Line",the Municipal Party Committee and the Municipal Government of Nanchong City decided to implement the " 300 Demonstration Projects" of modern agriculture. It firmly grasps the " bull nose"( key part) of the project,energetically implements the construction of 100 modern circular agricultural demonstration parks,100 agricultural leading enterprises with output value of 100 billion yuan,and 100 agricultural new business model demonstration projects( hereinafter referred to as 300 Demonstration Projects),to realize the goal of the output value of the modern agriculture industrial clusters of Nanchong City exceeds 100 billion yuan in 2020.

The effect of single-operation-hole thoracoscopic surgery on elderly solitary pulmonary nodules and its effects on lung function and prognosis

Objective:To investigate the effect of single-operation-hole thoracoscopic surgery on elderly solitary pulmonary nodules and its effects on lung function and prognosis.Methods:64 patients with solitary pulmonary nodules admitted to our hospital were selected. According to the different surgical treatment methods, they were divided into the study group and the control group, each group with 32 cases. The study group was treated with single-operation-hole thoracoscopic pulmonary nodule resection, and the control group was treated with conventional multi-operation-holes thoracoscopic pulmonary nodule resection. The clinical efficacy of the two groups was compared. Results: The postoperative drainage time, intraoperative blood loss, hospitalization time, postoperative drainage volume and hospitalization cost of the study group were significantly better than those of the control group (P<0.05), and there were no significant differences between the two groups in terms of the duration of operation and the incidence of adverse reactions (such as postoperative pulmonary atelectasis and pulmonary infection) (P>0.05). There was no difference in lung function between the two groups before operation (P>0.05). Compared with the control group, the lung function indexes of the postoperative study group were significantly improved (P<0.05). There was no difference in the overall survival rate and tumor-free survival rate of the two groups after operation for half a year (P>0.05). The VAS scores of the study group were significantly lower than those of the control group on the 1st and 3rd day after operation (P<0.05). There was no difference in the quality of life between the two groups before treatment (P>0.05), but the overall health status, physiological feature, physiological function, physical pain and energy of the patients in the study group after treatment were significantly higher than those in the control group (P<0.05), but there was no significant difference in social function, emotional function and mental health (P>0.05).Conclusions: Single-operation-hole thoracoscopic surgery is effective in the treatment of elderly solitary pulmonary nodules. It can not only accelerate the recovery of patients but also improve their lung function, without affecting the prognosis of patients, which can improve their quality of life. It is worthy of clinical application, but should strictly control the indications for surgery.

Effect of Fluorocarbon Side Chain on the Microphase Morphology and Rheological Behavior of Thermoplastic Polyurethanes

Fluorinated diols(FDO)with dangling chain were introduced into polyether urethanes(PEUs)as chain extender in order to achieve internal plasticization.Based on temperature dependent Fourier transform infrared(FTIR)spectroscopy results,the fluorinated polyether urethanes(FPEUs)exhibited weaker hydrogen bonding between C=O and N―H groups with addition of FDO.The crystallinity of hard domain was destroyed determined by thermal analysis.Combined with the results of dynamic mechanical analysis(DMA)and dissipative particle dynamics(DPD)simulation,it was proved that more loosely aggregated hard domains with weakened interaction separated from soft segment were formed.Rheological analysis indicated that FDO reduced the complex viscosity and viscous flow activation energy which endowed the polymer with more flexibility.Despite the loss of hydrogen bonds,there was no significant decline in mechanical property resulting from the decrease of hard segment dissolved in soft segment phase.The presence of fluorinated carbon side chain could function as internal plasticizer and contribute to the processability of polyurethane.