大流量斜拉压力输水管桥振动台模型试验研究

为了研究斜拉输水管桥的抗震性能,以某大跨径多塔斜拉输水管桥为对象,根据相似理论设计缩尺比为1∶20的等代试验模型,开展振动台试验研究.从不同地震波、不同台面输入地面峰值加速度以及空满管工况等多个角度探究斜拉输水管桥地震响应与抗震性能.采用试验与数值模拟相结合的方法,系统分析大跨径斜拉输水管桥的抗震性能.结果表明,E1地震下结构保持弹性;E2地震下部分钢筋进入屈服,混凝土出现裂缝但结构整体安全.纵桥向地震输入时,考虑流固耦合效应的满管工况相较于空管工况的地震响应增幅小于仅采用附加质量模拟水体时的响应增幅;横桥向地震输入时的结果相反.

多通道双晶单色器平行度测量与运动轨迹规划

针对双转轮多通道双晶单色器的平行度误差补偿问题,提出一种基于光电自准直仪的多通道双晶单色器准静态双晶衍射面平行度测量方法以及一种S形曲线轨迹规划方法。分析多通道单色器的结构及运动特点,基于光电自准直仪提出多通道双晶单色器平行度测量系统的总体框架及测量具体流程。结合多通道双晶单色器运动特点,提出一种S形曲线轨迹规划方法,并基于平行度测量结果对单色器运动轨迹进行优化设计。最后,在搭建的实验平台上开展准静态平行度测量实验及运动轨迹跟踪实验。结果表明:所搭建的平行度测量系统的平行度分辨率达0.5″,相邻两组晶体角度跃迁差值在百角秒量级;所提S形曲线轨迹规划算法将轨迹跟踪误差抑制在6.1″,相比传统5阶多项式及未规划轨迹,跟踪误差分别降低58.7%和63.9%。实验结果验证了所提方法有效性,对改进双转轮多通道双晶单色器性能具有良好的实际应用价值。

cAMP抑制核盘菌菌核形成的差异基因表达分析

【目的】探究核盘菌响应环磷酸腺苷(cyclic adenosine monophosphate,cAMP)处理的转录组学特征,挖掘调控核盘菌菌核形成的关键基因,为靶向杀菌剂的研发提供参考。【方法】以接种在PDA培养基上生长至菌核原基形成时期的核盘菌作为CK1,开始形成黑色素时期的核盘菌作为CK2,以接种在PDA+5 mmol/L cAMP培养基上生长相同时间(5,7 d)的核盘菌作为试验组,依次命名为M1和M2,对这4组样品进行转录组测序(RNA-seq)比较,阐释cAMP影响菌核形成的调控途径,挖掘菌核原基及菌核黑色素形成中的差异表达基因(differentially expressed genes,DEGs)。【结果】外源添加的cAMP主要在M1前期影响胞内的cAMP含量。差异表达基因分析结果显示,M1和CK1之间共有681个差异表达基因;KEGG富集分析显示,这些基因主要参与各类氨基酸代谢、脂肪酸代谢、MAPK信号通路等,且通路中的大部分基因都呈上调表达模式,而MAPK及其下游作用因子钙调磷酸酶B亚基(calcineurin B subunit,CnB)和热休克蛋白70(heat shock 70 ku protein,HSP70)则明显下调。M2和CK2之间共筛选到4490个差异表达基因,主要参与各类氨基酸代谢、抗原加工提呈以及cAMP、MAPK等信号通路和自噬途径,通路中的大部分基因呈上调表达模式,且自噬相关因子ATG2、ATG20、ATG22、ATG23、ATG27和ATG29也大量上调表达,而与蛋白质和脂类合成相关的磷酸酶B(phosphatase B,PTPB)和酰基辅酶A氧化酶(acyl coenzyme A oxidase,ACO)的表达量则明显下调。在M1和M2时期,相关基因的qRT-PCR与RNA-seq差异表达分析结果趋势相同,说明转录组测序数据准确。【结论】核盘菌可通过吸收胞外高浓度的cAMP抑制菌核形成菌丝,其抑制作用主要通过影响相关蛋白质及脂类物质的合成而对菌丝形态及信号传递造成障碍。

干旱胁迫下哈茨木霉对烟草幼苗生理指标的影响

为了探究干旱胁迫下哈茨木霉对烟草幼苗生理指标的影响,以烤烟品种K326为试验材料,采用蘸根法进行哈茨木霉处理,用不同浓度(10%、20%)聚乙二醇(PEG-6000)模拟不同程度(轻度、中度)的干旱胁迫,用沙培法研究不同程度干旱胁迫下哈茨木霉对烟草幼苗表型、叶片相对含水率、根系发育、光合色素含量、膜脂过氧化指标、渗透调节物质含量、保护酶活性的影响。结果表明,在正常清水处理条件下,哈茨木霉蘸根处理相比于清水条件更能够促进烟株生长,提高叶片相对含水率,促进根系发育,如总根长、投影面积、根表面积、分枝数、连接数等指标,增加烟叶中叶绿素a、叶绿素b含量,降低烟叶中过氧化氢、丙二醛含量,增加烟叶中脯氨酸、可溶性糖、可溶性蛋白含量,提升烟叶中过氧化氢酶、过氧化物酶、超氧化物歧化酶、抗坏血酸过氧化物酶活性。与清水对照相比,在轻度、中度干旱胁迫下,烟株变黄萎蔫,相对叶片含水率降低,总根长、投影面积、根表面积、连接数受到抑制,叶绿素a和叶绿素b含量减少,过氧化氢和丙二醛含量增加,脯氨酸、可溶性糖、可溶性蛋白含量增加,过氧化氢酶、过氧化物酶、超氧化物歧化酶、抗坏血酸过氧化物酶活性增加。在轻度和中度干旱的条件下,与同等程度干旱胁迫条件相比,哈茨木霉蘸根处理能够明显改善烟叶萎蔫变黄现象,增加叶片相对含水率,促进根系发育,如总根长、投影面积、根表面积、连接数,提高绿素a、叶绿素b、脯氨酸、可溶性糖、可溶性蛋白含量以及过氧化氢酶、过氧化物酶、超氧化物歧化酶、抗坏血酸过氧化物酶活性,降低过氧化氢和丙二醛含量。由研究结果可以看出,不同程度干旱胁迫均可对烟株造成不同程度的损伤,哈茨木霉蘸根处理可促进烟草发育、提高烟草的抗旱性,从而降低干旱胁迫对烟草造成的危害。

基于作战能力的模块化兵力抽组模型研究

为解决联合作战筹划兵力编组科学高效的问题,提出了作战元能力非线性生成聚合作战能力的方法,建立了“作战子任务-聚合作战能力-作战元能力-营级作战单元”的兵力抽组关系。以作战能力供需向量匹配为基本抽组依据,构建了多优化目标多约束条件的联合作战层级的模块化兵力抽组模型。提出了基于遗传算法的模型求解框架,说明了决策变量编码、适应值计算、兵力抽组解码的流程方法。实验表明:基于作战能力的模块化兵力抽组模型,能够精确高效地抽组满足作战任务需要的营级作战单元。

冀东中部稀有金属花岗伟晶岩的发现及意义

冀东地区位于华北克拉通北缘东段马兰峪复式背斜,是中国北方重要的内生金属成矿区。最近,在冀东中部新发现了一系列LCT型和NYF型稀有金属花岗伟晶岩,同空间伴生绢英岩化蚀变岩和稀有金属石英脉,成矿元素包括Ag、Au、Rb、Li、Nb、Zr、HREE、Th等。LCT型花岗伟晶岩包括锂云母/白云母花岗伟晶岩和天河石花岗伟晶岩,Rb_(2)O含量最高可达0.43%,最低为0.06%;Li_(2)O分布不均,仅在锂云母花岗伟晶岩中富集,最高可达0.33%。NYF型电气石花岗伟晶岩主要富集Nb,局部富集电气石,Nb含量为0.05%~0.06%,Ta含量很低,ThO_(2)含量为0.02%~0.05%。同空间的绢英岩化蚀变岩Rb_(2)O含量为0.06%~0.15%,Li_(2)O含量为0.07%~0.22%,局部含金,最高品位可达1.57×10^(-6);稀有金属石英脉,Nb_(2)O_(5)含量为0.06%~0.50%,富集Zr、重稀土、Th、U、Ag,其中Ag品位为18.2×10^(-6)~160.0×10^(-6)。初步地球化学分析认为,岩浆的成分变化以及岩浆-流体作用可能是研究区稀有金属花岗伟晶岩成矿的关键。

不同配方高碳基肥对靖西烤烟生长和产质量的影响

为探究不同配方高碳基肥对靖西烟区烟草生长和产质量的影响,确定一种适宜靖西烟区的高碳基有机肥配方。在靖西市进行大田试验,以云烟87为材料,试验设置4个处理:商品牛粪有机肥(CK);商品高碳基肥(T1),八角渣高碳基肥(T2),牛粪高碳基肥(T3),研究不同配方高碳基肥对烟草生长发育和产质量的影响。结果表明:在靖西市大田试验中,牛粪高碳基肥处理(T3)相比于商品牛粪有机肥处理(CK)株高提升5.28%,叶片增加1.05%,茎围增加1.14%,叶长、叶宽和叶面积分别增加3.09%、11.23%和14.67%;叶片酶活性及丙二醛含量中,商品高碳基肥处理(T1)酶活性相比于T2和T3处理较高,八角渣高碳基肥处理(T2)丙二醛含量最高;从外观质量评价来看,各处理总评分相差不大,高碳基肥有助于提高C3F等级烤后烟外观质量分数,商品高碳基肥处理(T1)相对较好;从烤后烟物理特性来看,牛粪高碳基肥处理(T3)表现最好,相比于商品牛粪有机肥处理(CK)上部叶的叶长和叶宽分别增加约2.00 cm和2.70 cm,含梗率降低1.40%,抗张力提高0.17 N/cm 2,中部叶的叶长和叶宽分别增加1.50 cm和3.00 cm,含梗率降低1.70%,抗张力提高0.17 N/cm 2;从烤后烟化学成分来看,八角渣高碳基肥处理(T2)相比于商品牛粪有机肥处理(CK)B2F、C3F等级烤后烟的总糖分别增加6.75%和6.99%,还原糖分别增加6.60%和7.29%,钾含量提高0.13%和0.04%,糖碱比分别增加27.04%和34.17%;从产量产值来看,八角渣高碳基肥处理(T2)为最优处理,亩产量达到141.88 kg/亩,亩产值达到3809.48元/亩,均价达到26.85元/kg,上等烟比例达到62.87%。综合靖西市大田试验中不同处理烟草生长发育和产质量分析,八角渣高碳基肥施肥方式(T2)更适宜在靖西烟区推广。

Current landscape of preoperative neoadjuvant therapies for initial resectable colorectal cancer liver metastasis

Colorectal cancer liver metastasis(CRLM)presents a clinical challenge,and optimizing treatment strategies is crucial for improving patient outcomes.Surgical resection,a key element in achieving prolonged survival,is often linked to a heightened risk of recurrence.Acknowledging the potential benefits of preoperative neoadjuvant chemotherapy in managing resectable liver metastases,this approach has gained attention for its role in tumor downsizing,assessing biological behavior,and reducing the risk of postoperative recurrence.However,the use of neoadjuvant chemotherapy in initially resectable CRLM sparks ongoing debates.The balance between tumor reduction and the risk of hepatic injury,coupled with concerns about delaying surgery,necessitates a nuanced approach.This article explores recent research insights and draws upon the practical experiences at our center to address critical issues regarding considerations for initially resectable cases.Examining the criteria for patient selection and the judicious choice of neoadjuvant regimens are pivotal areas of discussion.Striking the right balance between maximizing treatment efficacy and minimizing adverse effects is imperative.The dynamic landscape of precision medicine is also reflected in the evolving role of gene testing,such as RAS/BRAF and PIK3CA,in tailoring neoadjuvant regimens.Furthermore,the review emphasizes the need for a multidisciplinary approach to navigate the comp-lexities of CRLM.Integrating technical expertise and biological insights is crucial in refining neoadjuvant strategies.The management of progression following neoadjuvant chemotherapy requires a tailored approach,acknowledging the diverse biological behaviors that may emerge.In conclusion,this review aims to provide a comprehensive perspective on the considerations,challenges,and advancements in the use of neoadjuvant chemotherapy for initially resectable CRLM.By combining evidencebased insights with practical experiences,we aspire to contribute to the ongoing discourse on refining treatment paradigms for improved outcomes in patients with CRLM.

The state-of-the-art of atmospheric pressure plasma for transdermal drug delivery

Plasma-enhanced transdermal drug delivery(TDD) presents advantages over traditional methods,including painless application, minimal skin damage, and rapid recovery of permeability. To harness its clinical potential, factors related to plasma’s unique properties, such as reactive species and electric fields, must be carefully considered.This review provides a concise summary of conventional TDD methods and subsequently offers a comprehensive examination of the current state-of-the-art in plasma-enhanced TDD. This includes an analysis of the impact of plasma on HaCaT human keratinocyte cells, ex vivo/in vivo studies, and clinical research on plasma-assisted TDD. Moreover, the review explores the effects of plasma on skin physical characteristics such as microhole formation, transepidermal water loss(TEWL), molecular structure of the stratum corneum(SC), and skin resistance. Additionally, it discusses the involvement of various reactive agents in plasma-enhanced TDD, encompassing electric fields,charged particles, UV/VUV radiation, heat, and reactive species. Lastly, the review briefly addresses the temporal behavior of the skin after plasma treatment, safety considerations, and potential risks associated with plasma-enhanced TDD.

A Blockchain-Based Efficient Cross-Domain Authentication Scheme for Internet of Vehicles

The Internet of Vehicles(IoV)is extensively deployed in outdoor and open environments to effectively address traffic efficiency and safety issues by connecting vehicles to the network.However,due to the open and variable nature of its network topology,vehicles frequently engage in cross-domain interactions.During such processes,directly uploading sensitive information to roadside units for interaction may expose it to malicious tampering or interception by attackers,thus compromising the security of the cross-domain authentication process.Additionally,IoV imposes high real-time requirements,and existing cross-domain authentication schemes for IoV often encounter efficiency issues.To mitigate these challenges,we propose CAIoV,a blockchain-based efficient cross-domain authentication scheme for IoV.This scheme comprehensively integrates technologies such as zero-knowledge proofs,smart contracts,and Merkle hash tree structures.It divides the cross-domain process into anonymous cross-domain authentication and safe cross-domain authentication phases to ensure efficiency while maintaining a balance between efficiency and security.Finally,we evaluate the performance of CAIoV.Experimental results demonstrate that our proposed scheme reduces computational overhead by approximately 20%,communication overhead by around 10%,and storage overhead by nearly 30%.

西藏冈底斯南缘阿扎乡古新世花岗闪长岩地质特征及成矿潜力评价

西藏冈底斯岩浆弧带是我国重要的铜矿资源产地。研究区位于南冈底斯斑岩铜矿带东段克鲁铜金矿床西侧,古新世岩浆活动较为强烈,但如此强烈的岩浆活动与铜矿的关系究竟如何?其是否具有好的成矿潜力?解决这些问题对指导该地区的找矿工作具有重要的意义。基于此,本文通过年代学、岩石地球化学、锆石同位素分析了花岗闪长岩的岩浆起源、岩石成因及形成的构造环境,并结合氧逸度分析成矿潜力。LA-ICP-MS锆石U-Pb定年结果显示,岩体形成于古新世(64.55±0.93 Ma),岩石属钙碱性系列,稀土元素总量∑REE(包括Y元素)平均为94.01×10^(-6),富集K、U、Rb、Ba和轻稀土(LREEs),亏损Nb、Ta、Ti、Zr和重稀土(HREEs),无明显Eu负异常。Rb/Sr比值平均为0.20,Nb/Ta比值平均为11.89,锆石ε_(Hf)(t)值为+10.7~+13.3,t_(DM2)变化于285.5~453.4 Ma,I_(A)=490,I_(T)=484,岩体中锆石结晶温度大约在700℃,显示岩浆来源于俯冲环境新生地壳的部分熔融,并有少量幔源物质加入,具典型的I型花岗岩特征,推断其形成于挤压俯冲碰撞前的弧构造环境。结合高氧逸度、地幔物质以及流体的贡献,可推知该地区具有良好的深部铜金资源成矿潜力。

Left ventricular thrombosis caused cerebral embolism during venoarterial extracorporeal membrane oxygenation support: A case report

BACKGROUND Venoarterial(VA)extracorporeal membrane oxygenation(ECMO),an effective short-term circulatory support method for refractory cardiogenic shock,is widely applied.However,retrospective analyses have shown that VA-ECMO-assisted cases were associated with a relatively high mortality rate of approximately 60%.Embolization in important organs caused by complications of left ventricular thrombosis(LVT)during VA-ECMO is also an important reason.Although the incidence of LVT during VA-ECMO is not high,the consequences of embolization are disastrous.CASE SUMMARY A 37-year-old female patient was admitted to hospital because of fever for 4 d and palpitations for 3 d.After excluding the diagnosis of coronary heart disease,we established a diagnosis of“clinically explosive myocarditis”.The patient still had unstable hemodynamics after drug treatment supported by VA-ECMO,with heparin for anticoagulation.On day 4 of ECMO support,a left ventricular thro-mbus attached to the papillary muscle root of the mitral valve was found by transthoracic echocardiography.Left ventricular decompression was performed and ECMO was successfully removed,but the patient eventually died of multiple cerebral embolism.CONCLUSION LVT with high mobility during VA-ECMO may cause embolism in important organs.Therefore,a"wait and see"strategy should be avoided.

Small cell lung carcinoma with KIF5B-RET fusion partially responded to the 4^(th)-line therapy with anlotinib:A case report

BACKGROUND Small cell lung cancer(SCLC)exhibits a pronounced tendency for metastasis and relapse,and the acquisition of resistance to chemotherapy and radiotherapy,leading to complexity in treatment outcomes.It is crucial to tackle these challenges by advancing targeted therapeutic approaches in ongoing research endeavors.Variant RET fusions have been reported in several solid tumors,but are rarely reported in SCLC.CASE SUMMARY We present the first case of a KIF5B-RET fusion in a 65-year-old male patient with SCLC.To date,the patient has received the 4th line chemotherapy with anlotinib for one year and has shown a sustained favorable partial response.According to the results of next generation sequencing,this SCLC patient harbors the KIF5BRET fusion,suggesting that RET fusion could serve as a promising molecular target for SCLC treatment.Next-generation sequencing(NGS)plays a critical rolein comprehensively assessing the genotype and phenotype of cancer.CONCLUSION NGS can provide SCLC patients with personalized and targeted therapy options,thereby improving their likelihood of survival.

Regenerative Braking Energy Recovery System of Metro Train Based on Dual-Mode Power Management

In order to fully utilize the regenerative braking energy of metro trains and stabilize the metro DC traction busbar voltage,a hybrid regenerative braking energy recovery system with a dual-mode power management strategy is proposed.Firstly,the construction of the hybrid regenerative braking energy recovery system is explained.Then,based on the power demand of low-voltage load in metro stations,a dual-mode power management strategy is proposed to allocate the reference power of each system according to the different working conditions,and the control methods of each system are set.Finally,the correctness and effectiveness of the dual-mode strategy are verified through simulation,and the proposed braking energy utilization scheme is compared with other singleform utilization schemes.The results illustrate that the hybrid system with the dual-mode strategy can effectively recycle the regenerative braking energy of metro train and inhibit the busbar voltage fluctuation;the proposed braking energy utilization scheme has certain advantages on energy recovery and DC bus voltage stabilization compared with other single-form schemes;the proposed power management strategy can correctly allocate the reference power of each system with a lower construction cost.

An Adaptive Control Strategy for Energy Storage Interface Converter Based on Analogous Virtual Synchronous Generator

In the DC microgrid,the lack of inertia and damping in power electronic converters results in poor stability of DC bus voltage and low inertia of the DC microgrid during fluctuations in load and photovoltaic power.To address this issue,the application of a virtual synchronous generator(VSG)in grid-connected inverters control is referenced and proposes a control strategy called the analogous virtual synchronous generator(AVSG)control strategy for the interface DC/DC converter of the battery in the microgrid.Besides,a flexible parameter adaptive control method is introduced to further enhance the inertial behavior of the AVSG control.Firstly,a theoretical analysis is conducted on the various components of the DC microgrid,the structure of analogous virtual synchronous generator,and the control structure’s main parameters related to the DC microgrid’s inertial behavior.Secondly,the voltage change rate tracking coefficient is introduced to adjust the change of the virtual capacitance and damping coefficient flexibility,which further strengthens the inertia trend of the DC microgrid.Additionally,a small-signal modeling approach is used to analyze the approximate range of the AVSG’s main parameters ensuring system stability.Finally,conduct a simulation analysis by building the model of the DC microgrid system with photovoltaic(PV)and battery energy storage(BES)in MATLAB/Simulink.Simulation results from different scenarios have verified that the AVSG control introduces fixed inertia and damping into the droop control of the battery,resulting in a certain level of inertia enhancement.Furthermore,the additional adaptive control strategy built upon the AVSG control provides better and flexible inertial support for the DC microgrid,further enhances the stability of the DC bus voltage,and has a more positive impact on the battery performance.

Power Quality Disturbance Identification Basing on Adaptive Kalman Filter andMulti-Scale Channel Attention Fusion Convolutional Network

In light of the prevailing issue that the existing convolutional neural network(CNN)power quality disturbance identification method can only extract single-scale features,which leads to a lack of feature information and weak anti-noise performance,a new approach for identifying power quality disturbances based on an adaptive Kalman filter(KF)and multi-scale channel attention(MS-CAM)fused convolutional neural network is suggested.Single and composite-disruption signals are generated through simulation.The adaptive maximum likelihood Kalman filter is employed for noise reduction in the initial disturbance signal,and subsequent integration of multi-scale features into the conventional CNN architecture is conducted.The multi-scale features of the signal are captured by convolution kernels of different sizes so that the model can obtain diverse feature expressions.The attention mechanism(ATT)is introduced to adaptively allocate the extracted features,and the features are fused and selected to obtain the new main features.The Softmax classifier is employed for the classification of power quality disturbances.Finally,by comparing the recognition accuracy of the convolutional neural network(CNN),the model using the attention mechanism,the bidirectional long-term and short-term memory network(MS-Bi-LSTM),and the multi-scale convolutional neural network(MSCNN)with the attention mechanism with the proposed method.The simulation results demonstrate that the proposed method is higher than CNN,MS-Bi-LSTM,and MSCNN,and the overall recognition rate exceeds 99%,and the proposed method has significant classification accuracy and robust classification performance.This achievement provides a new perspective for further exploration in the field of power quality disturbance classification.

Automatic SOC Equalization Strategy of Energy Storage Units with DC Microgrid Bus Voltage Support

In this paper,an improved sag control strategy based on automatic SOC equalization is proposed to solve the problems of slow SOC equalization and excessive bus voltage fluctuation amplitude and offset caused by load and PV power variations in a stand-alone DC microgrid.The strategy includes primary and secondary control.Among them,the primary control suppresses the DC microgrid voltage fluctuation through the I and II section control,and the secondary control aims to correct the P-U curve of the energy storage system and the PV system,thus reducing the steady-state bus voltage excursion.The simulation results demonstrate that the proposed control strategy effectively achieves SOC balancing and enhances the immunity of bus voltage.The proposed strategy improves the voltage fluctuation suppression ability by approximately 39.4%and 43.1%under the PV power and load power fluctuation conditions,respectively.Furthermore,the steady-state deviation of the bus voltage,△U_(dc) is only 0.01–0.1 V,ensuring stable operation of the DC microgrid in fluctuating power environments.

改良的RIHA方法定量检测PCV2 Cap抗原的研究

为提高反向间接血凝试验(reverse indirect hemagglutination assay,RIHA)定量检测PCV2 Cap抗原的稳定性和精准性,将RIHA方法改良为:1)将被检样品稀释为几个适宜稀释度,分别做RIHA检测;2)检测孔100%、75%、50%、25%、0%凝集分别记录为4、3、2、1、0分,将几个稀释度的被检样品全部检测孔的分数加和;3)同法检测参照品并将参照品分数加和;4)被检样品与参照品总分数对比计算,获得被检样品抗原含量。改良的RIHA方法进行稳定性评价并与ELISA方法做比较。结果显示,改良RIHA方法的批内差异为6.8%~19.4%、批间差异为11.4%~23.6%、检测同一个样品差异系数为8.5%,均优于常规RIHA方法。改良RIHA方法检测纯化前和纯化后的PCV2 Cap抗原共30份,与ELISA方法检测结果比较相关系数为0.9568。研究结果表明,改良的PCV2 Cap抗原RIHA定量检测方法稳定性良好,且与ELISA方法检测结果有相关性,可在PCV2 Cap抗原生产中做定量检测,也可在毒种筛选、生产工艺优化中快速定量检测PCV2 Cap抗原含量。

一株猫疱疹病毒Ⅰ型(FHV-1)的分离鉴定及攻毒模型建立

从一只临床表现为流脓涕、打喷嚏的猫身上分离出一株猫疱疹病毒Ⅰ型(Feline herpesvirus typeⅠ,FHV-1),使用F81细胞进行分离,并通过PCR、透射电镜和糖蛋白D(gD)基因序列分析来鉴定病毒。通过PCR方法从猫的鼻分泌物中扩增出1125 bp的gD基因。序列分析表明,该分离株与从GenBank获得的其他FHV-1毒株属于同一家族。随后在接种猫鼻分泌物的F81细胞的培养上清中观察到有包膜和直径约120 nm左右的疱疹样病毒颗粒。将分离获得的FHV-1病毒感染2~3月龄蓝猫,成功建立动物实验模型,感染后的猫出现脓性的眼鼻分泌物,打喷嚏等临床症状,且一周内持续排毒。该分离株为FHV-1的病原学研究提供了重要的数据,也为猫鼻气管炎疫苗候选株的筛选和疫苗的研发奠定了基础。

SARS-CoV-2 Infection Caused COVID-19 Related Otitis Media Is Commonly Existed with Good Prognosis

Otitis media with effusion is a common disease in otolaryngology. Bacteria are the most common pathogen of otitis media with effusion, and other factors such as viruses have also been reported. The present study is aimed to investigate whether the increasing otitis media cases recently is correlated with Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection and the COVID-19-related otitis media is commonly existed. Thirty-two patients with otitis media were enrolled Blood cell analysis, C-reactive protein, interleukin-6 test bacterial and fungal cultures were tested. Nine patients were identified with positive SARS-CoV-2 RNA in middle ear discharge. All the subjects had the common symptoms of stuffy ear, hearing loss, and echoacousia. No positive results were found in cultures for bacterial and fungus of middle ear discharge. The levels of C-reactive protein (CRP) were significantly up-regulated in positive cases (P = 0.0335). The levels of proinflammatory cytokine interleukin-6 (IL-6) were higher in positive cases. There were no significant differences of age, gender and prothrombin time (PT) between positive and negative cases. Nasal sprays, ciliary stimulants, and prophylactic antibiotics or low-dose steroid treatments were sequentially used in the otitis media patients caused by SARS-CoV-2 infection. All the patients had improvements of typical symptoms within two to four weeks during the following-up. Thus, SARS-CoV-2 infection caused COVID-19 related otitis media is commonly existed, and the prognosis is good after treatments.