Advances in Research on Medicinal Plants of Lindernia All. in Guangxi Province

On the basis of extensive literature search, the paper summarized the pharmacological identification, chemical composition, pharmacological effects and clinical application of Lindernia plants produced in Guangxi Province, with an attempt to provide basic information for further development and utilization of the medicinal resources.

Network Curriculum Design of Traditional Chinese Medicine Pharmacy Specialty Formed by Introducing Guangxi Minority Medicine

Starting from the background and significance of the development and design of the network course of traditional Chinese medicine pharmacy specialty formed by introducing Guangxi minority medicine,this paper discusses the teaching design scheme and module construction of the network course in the development process,and summarizes the design,production and construction experience of the network course.Through practice,the teaching effect and achievement of the network course of traditional Chinese medicine pharmacy specialty formed by introducing Guangxi minority medicine are obvious.

On the HPLC Fingerprint of Zhuang Medicine Spilanthes paniculata Wall. ex DC.

[Objectives] To establish the HPLC fingerprint of Spilanthes paniculata Wall. ex DC. and provide reference for the quality control of medicinal materials. [Methods]Welch Ultimate XB-C18( 5 μm,250 mm ×4. 6 mm) chromatographic column was adopted,and the mobile phase was acetonitrile-0. 1% phosphoric acid aqueous solution for gradient elution,the detection wavelength was 328 nm,the column temperature was 25 ℃,the similarities of 18 different batches of medicinal materials were evaluated,and the HPLC fingerprint chromatogram of S. paniculata Wall. ex DC. was set up. [Results] The RSDs of precision,repeatability and stability of HPLC fingerprint of 18 batches of S. paniculata Wall. ex DC. medicine materials were all less than 3%;HPLC reference fingerprint of the medicine materials was established.[Conclusions]HPLC fingerprint was established,its data were stable and reliable,and the method was simple and efficient,which could provide reference for the quality evaluation of Zhuang medicine S. paniculata Wall. ex DC.

SSR Primer Screening for Marchantia convoluta by SAM Method

[Objectives] This study was conducted to study SSR primers in wild Marchantia convoluta by SAM method. [Methods] The M. convoluta genomic DNA was extracted by improved CTAB method,and the SSR primers for M. convoluta were selected through digestion,ligation,suppression PCR,pre-amplification,SAM method and PCR. [Results] The improved CTAB method extracted a high purity of DNA; the SAM PCR method gave clear bands; and 100 positive clones were sequenced,obtaining 86 sequences. [Conclusions] The SSR molecular markers could be used for analyzing the genetic diversity of M. convoluta.

Research Progress of Total Flavones in Marchantia convoluta

According to the literature retrieval,the composition,detection method,preparation method and product development of total flavones in Marchantia convoluta were reviewed and prospected. This paper will provide a theoretical basis for further study and product development of total flavones in M. convoluta.

Difference Analysis of Secondary Metabolites of Herba Polygoni Chinensis from Guangxi

[Objectives]The differences in secondary metabolites of Herba Polygoni Chinensis from Guangxi were analyzed,in order to find flavonoids which can antagonize liver injury and provide a basis for the quality evaluation of Herba Polygoni Chinensis.[Methods]UPLC-MS was used to analyze the secondary metabolites of Herba Polygoni Chinensis,and emphasis was placed on flavonoids.[Results]Flavonoids were abundant in the secondary metabolites of Herba Polygoni Chinensis.Quercitrin,catechin gallate,epicatechin gallate,hyperoside and so on were screened as the components with high content and antagonistic effect on liver injury.[Conclusions]Quercitrin was the flavonoid compound with the highest content among the three origins.It is stable and has a good protective effect on liver,which is representative.Quercitrin can be considered as the target component to be used as an important index for quality evaluation of Herba Polygoni Chinensis.

Spectral Characteristics and Quantitative Analysis of Troxerutin-Cu^2+ Complex

[Objectives]This study aims to establish a simple,convenient,fast,low-cost,low-consumption and efficient method for quality control of troxerutin.[Methods]In NaAc-phosphate buffer at pH=8,troxerutin could react with Cu^2+to form complex,which had a characteristic absorption peak at 408 nm in the ultraviolet spectrum.The content of troxerutin could be determined indirectly by measuring the absorbance of the complex.[Results]Within the linear range of 27.82-74.19μg/mL,the absorbance of the complex has a good linear relationship with the concentration of troxerutin.The regression equation is A=0.0119C-0.1384(r=0.9991),and the detection limit is 1.59μg/mL.This method used to determine the content of troxerutin in samples from different manufacturers,and the recovery is 100.9%-102.7%.[Conclusions]The ultraviolet spectrophotometry is used in this experiment to indirectly determine the content of troxerutin by measuring the absorbance of troxerutin-Cu^2+complex,and the results are accurate,reproducible and highly precise.This method can be used for quantitative analysis of troxerutin.

Safety Limit Test of Heavy Metals and Pesticide Residues in the Zhuang Ethnic Medcine Herba Polygoni Chinensis from 13 Producing Areas

[Objectives]This study was conducted to determine the contents of such five heavy metals as lead(Pb),cadmium(Cd),arsenic(As),mercury(Hg)and copper(Cu)and the contents of nine kinds of organochlorine pesticide residues including total BHC(α-BHC,β-BHC,γ-BHC,δ-BHC)and total DDT(pp-DDE,pp-DDD,op-DDT,pp-DDT)and pentachloronitrobenzene in the Zhuang medicine Herba Polygoni Chinensis from 13 producing areas.[Methods]The five heavy metals were determined in accordance with the second method of general rule 2341,the inductively coupled plasma mass spectrometry method,in part 4 of the 2020 edition of Chinese Pharmacopoeia;and the nine organochlorines were determined in accordance with the first method of general rule 2341,organochlorine pesticide residue determination method(chromatography),in part 4 of the 2020 edition of Chinese Pharmacopoeia.[Results]In the Herba Polygoni Chinensis from 13 origins,the lead contents were in the range of 0.50-3.28 mg/kg;the cadmium contents were in the range of 0.06-4.21 mg/kg;the arsenic contents were in the range of 0.16-6.9 mg/kg;the copper contents were in the range of 10.5-45.1 mg/kg;the mercury contents were in the range of 0.00-0.003 mg/kg;and the nine organochlorines including the total benzene hexachloride(α-BHC,β-BHC,γ-BHC,δ-BHC),total DDT(pp-DDE,pp-DDD,op-DDT,pp-DDT)and pentachloronitrobenzene were all not detected.Referring to part 4 of the 2020 edition of Chinese Pharmacopoeia,the Cu and As of the Herba Polygoni Chinensis produced in Hezhou exceeded the standards,the Cu of the Herba Polygoni Chinensis produced in Wuzhou exceeded the standard,and the As of the Herba Polygoni Chinensis produced in Yulin City and Guilin City exceeded the standard.[Conclusions]This study provides safety limit test data for the development and utilization of Herba Polygoni Chinensis of the Zhuang medicine.

Analysis of the Volatile Components of Polygonum chinense L.Extracted with Different Solvents by GC-MS

[Objectives]This study was conducted to investigate the volatile components of Polygonum chinense L.[Methods]The volatile components of the methanol extract,ethyl acetate extract,chloroform extract and petroleum ether extract of P.chinense were analyzed and determined by gas chromatography.[Results]The volatile components of the methanol extract from P.chinense were the most,and phenolic acids and ketones accounted for a relatively high proportion,among which pyrogallic acid had the highest content.The ethyl acetate extract contained the second most volatile components,mostly acids and esters,of whichβ-sitosterol had the highest content;the chloroform and petroleum ether extracts had relatively few types of volatile components,most of which were alkanes,and the content ofγ-sitosterol shared by the two was the highest.The common substances of the four extracts were palmitic acid and phytol.[Conclusions]This study provides a theoretical basis for the quality evaluation of P.chinense and a scientific basis for its resource development.

Synthesis Design and Research Progress of MOF Materials in Biomedicine

Compared with traditional materials,metal-organic framework materials(MOFs)have the characteristics of drug release controllability,degradability,designability and adjustability of composition and structure,excellent load capacity,and designability and adjustability of channel shape and size,and have shown a wide range of application value in the field of biomedicine.In this paper,based on the structural characteristics of MOFs,the synthetic design of MOF materials was expounded,and the research achievements of MOF materials in biomedicine in recent years were reviewed.

Research Progress of Herb Pair Rhizoma Coptidis-Fructus Evodiae

The herb pair is the basic form of compatibility and application of traditional Chinese medicine.Herb pairs have the advantages of simple structure,clear compatibility and effective characteristics,and are suitable for scientific research.Rhizoma Coptidis-Fructus Evodiae is a relatively fixed combination of two herbs commonly used in clinical Chinese medicine.Chinese medicine uses it to treat various diseases caused by damp-heat accumulation in the liver and gallbladder,and spleen and stomach.The two medicines in the herb pair are opposite in nature but complement each other,and can achieve a great effect based on simple combination.Modern research shows that the herb pair has a wide range of pharmacological effects and good anti-tumor,analgesic,bacteriostatic and anti-inflammatory activity,and show good curative effects on stress ulcers,functional dyspepsia,chronic colitis,etc.This paper reviewed the research progress on the herb pair of Rhizoma Coptidis-Fructus Evodiae.

Discussion on Ideological and Political Theories Teaching in Traditional Chinese Medicine Courses Based on National Standard for Teaching Quality

This paper analyzes the necessity and existing problems of ideological and political theories teaching in traditional Chinese medicine courses,and puts forward some suggestions,such as improving the ideological and political ability of professional teachers,perfecting the evaluation mechanism,and exploring ideological and political elements based on the characteristics of courses,so as to provide the basis for ideological and political theories teaching in traditional Chinese medicine courses under the National Standard for Teaching Quality.

Study on Planting and Cultivation Techniques of Polygonatum

Polygonatum is a food having the concomitant function of both medicine and foodstuff.It is relatively common in the tonic medicinal material market and is a common bulk medicinal material in China.Because of its good medicinal value and health care value,its artificial cultivation has become a hot topic,in order to increase market demand.The cultivation techniques of Polygonatum were discussed and analyzed by collecting and consulting data,as well as practical investigation.

Research on the Development of Guangxi Zhuang and Yao Ethnic Medicine Industry

The Chinese medicine revitalization plan represented by Zhuang and Yao ethnic medicine has been incorporated into China’s and Guangxi’s overall economic and social development plans,reflecting that The Zhuang and Yao ethnic medicine industry is an important part of China’s ethnic medicine,and it is also the representative ethnic medicine industry in Guangxi.In this paper,we investigated the development of Zhuang and Yao ethnic medicine industry and its development status by consulting literature,documents and related materials and visiting relevant departments,systematically analyzed the advantages and disadvantages of the development of Guangxi Zhuang and Yao ethnic medicine industry,and put forward feasible suggestions,aiming to accelerate the healthy development of Guangxi Zhuang and Yao ethnic medicine industry and provide effective data and decision-making basis for China to formulate relevant policies.

Advances in the Treatment of Hepatitis with Flavonoids

Flavonoids are important compounds in natural medicines.They have a wide range of pharmacological activity and have important research value and development prospects.In this article,literature on the treatment of hepatitis with flavonoids is searched,and research reports on the treatment of hepatitis with flavonoids in the past ten years are sorted out,in order to provide a basis for the in-depth research and further development of flavonoids in the treatment of hepatitis.

Preliminary Study on the Training Mode of Chinese Pharmacy Professionals Based on the National Standard of Teaching Quality

With the promulgation of the National Standard for the Teaching Quality of Undergraduate Majors in Ordinary Colleges and Universities, the state has made clear regulations on the standard for the cultivation of Chinese pharmacy talents. Based on their own actual conditions, major colleges and universities have implemented different talent training modes, including talent training modes of running talent classes, "2+1+1", "Chinese Medicine Research Practice Class", employment-oriented professional talent training mode, three-level three-type diversified talent training mode, and talent training mode oriented with adapting to the characteristics of ethnic regions. Various talent training modes have different training objectives, and corresponding training programs are formulated to meet the requirements of the National Standard for the Teaching Quality of Undergraduate Majors in Ordinary Colleges and Universities. In addition, according to the notice on Solidly Promoting Labor Education into the "National Standard for the Teaching Quality of Undergraduate Majors in Ordinary Colleges and Universities" issued by the Department of Higher Education of the Ministry of Education, the introduction of labor education to the teaching of Chinese pharmacy majors will be a trend, and Chinese pharmacy professionals are required to achieve comprehensive and coordinated development of morality, intelligence, physique, beauty, and labor. How to integrate labor education with high quality and high efficiency into the training of Chinese pharmacy professionals is a problem that major universities will think about.

Research and Practice on Curriculum Reform of Chinese Pharmaceutical Manufacturing Major against the Backdrop of"Comprehensive Health"

Against the backdrop of Comprehensive Health,the Chinese Pharmaceutical Manufacturing major faces both opportunities and challenges.This paper describes some problems encountered in the development of the Chinese Pharmaceutical Manufacturing major,puts forward some suggestions and measures to adapt to the training of talents of Chinese pharmaceutical manufacturing against the backdrop of Comprehensive Health,and discusses the practical research on curriculum reform.

Flavonoid moiety-incorporated carbon dots for ultrasensitive and highly selective fluorescence detection and removal of Pb^2＋

A new type of self-targeting carbon dot （CD-Fla） for the detection of the toxic heavy metal ion Pb^2＋ was synthesized via a one-pot hydrothermal route using flavonoid extracts of Ginkgo biloba leaves as the starting material. As-prepared CD-Fla exhibited excellent biocompatibility and strong blue emission with a quantum yield of 16.1% and significant fluorescence quenching selectivity for Pb^2＋ without using any additional targeting molecules. CD-Fla could detect Pb^2＋ quantitatively within the range 0.1-20.0 nM, with an ultrahigh sensitivity of 55 pM. The selectivity of CD-FIa for Pb^2＋ was nearly one order of magnitude higher than that for other relevant metal ions. This was much better than ever reported CD-based metal ion sensors. The high sensitivity and selectivity were due to the incorporation of certain flavonoid-like moieties into CD-Fla. CD-Fla was also demonstrated to be a good probe for fluorescence tracing of intracellular Pb^2＋. The capability of CD-Fla was further improved when it was doped on agarose hydrogel. CD-Fla-doped agarose hydrogel （CD-AHG） allowed for visual fluorescence detection and removal of Pb^2＋ from water. This was confirmed by testing CD-AHG in actual water samples taken from the Jialing River （Chongqing, China）. The Pb^2＋ adsorbed CD-AHG was regenerable in HC1 solution. This study will open a new avenue for synthesizing intelligent materials capable of simultaneously targeting, detecting, and treating heavy metal ions.