Synthetic N-heterocyclic compounds,such as quinoxalines,have shown a crucial role in pharmaceutical as well as food and dye industries.However,the traditional synthesis toward N-heterocycles relies on multistep energy...Synthetic N-heterocyclic compounds,such as quinoxalines,have shown a crucial role in pharmaceutical as well as food and dye industries.However,the traditional synthesis toward N-heterocycles relies on multistep energy and costintensive non-sustainable processes.Here,we report a facile approach that allows one-step conversion of biomass-derived carbohydrates to valuable quinoxalines in the presence of aryl-1,2-diamines in water without any harmful metal catalysts/organic solvents via spontaneously engineering involved cascade reactions under hydrothermal conditions.Aryl-1,2-diamines are revealed as the key to propel this transformation through boosting carbohydrate fragmentation into small 1,2-dicarbonyl intermediates and subsequently trapping them for constituting stable quinoxaline scaffolds therefore avoiding a myriad of undesired side reactions.The tunability of product selectivity can be also achievable by adjusting the basicity of the reaction environment.Both batch and continuous-flow integrated processes were verified for production of quinoxalines in an exceptionally eco-benign manner(E-factor<1),showing superior sustainability and economic viability.展开更多
Nasopharyngeal carcinoma(NPC)is a malignant tumor that usually occurs in people from Southeast Asia and Southern China.NPC is prone to migration and invasion,leading to poor prognosis.A large number of circular RNAs(c...Nasopharyngeal carcinoma(NPC)is a malignant tumor that usually occurs in people from Southeast Asia and Southern China.NPC is prone to migration and invasion,leading to poor prognosis.A large number of circular RNAs(circ RNAs)exacerbate the process of metastasis in NPC;however,their underlying mechanisms remain unclear.We found that the circular RNA circ CCNB1,encoded by the oncogene CCNB1,was downregulated in NPC biopsies and cell lines.In vitro assays show that circ CCNB1 inhibits NPC cell migration and invasion.Moreover,circ CCNB1 induces a protein,nuclear factor 90(NF90),to bind and prolong the half-life of tight junction protein 1(TJP1)m RNA.Upregulation of TJP1 enhances tight junctions between cancer cells and inhibits NPC cell migration and invasion.This study reveals a novel biological function of circ CCNB1 in the migration and invasion of NPC by enhancing the tight junctions of cancer cells by binding to NF90 proteins and TJP1 m RNA,and may provide a potential therapeutic target for NPC.展开更多
基金supported by the National Natural Science Foundation of China(21932006)the China Postdoctoral Science Foundation(2019M652058)
文摘Synthetic N-heterocyclic compounds,such as quinoxalines,have shown a crucial role in pharmaceutical as well as food and dye industries.However,the traditional synthesis toward N-heterocycles relies on multistep energy and costintensive non-sustainable processes.Here,we report a facile approach that allows one-step conversion of biomass-derived carbohydrates to valuable quinoxalines in the presence of aryl-1,2-diamines in water without any harmful metal catalysts/organic solvents via spontaneously engineering involved cascade reactions under hydrothermal conditions.Aryl-1,2-diamines are revealed as the key to propel this transformation through boosting carbohydrate fragmentation into small 1,2-dicarbonyl intermediates and subsequently trapping them for constituting stable quinoxaline scaffolds therefore avoiding a myriad of undesired side reactions.The tunability of product selectivity can be also achievable by adjusting the basicity of the reaction environment.Both batch and continuous-flow integrated processes were verified for production of quinoxalines in an exceptionally eco-benign manner(E-factor<1),showing superior sustainability and economic viability.
基金the National Natural Science Foundation of China(82002239,82072374 and 82073135)the Overseas Expertise Introduction Project for Discipline Innovation(111 Project,111-2-12)+1 种基金the Natural Science Foundation of Hunan Province(2021JJ41043 and 2021JJ30897)Central South University Graduate Research and Innovation Project(2021zzts0310)。
文摘Nasopharyngeal carcinoma(NPC)is a malignant tumor that usually occurs in people from Southeast Asia and Southern China.NPC is prone to migration and invasion,leading to poor prognosis.A large number of circular RNAs(circ RNAs)exacerbate the process of metastasis in NPC;however,their underlying mechanisms remain unclear.We found that the circular RNA circ CCNB1,encoded by the oncogene CCNB1,was downregulated in NPC biopsies and cell lines.In vitro assays show that circ CCNB1 inhibits NPC cell migration and invasion.Moreover,circ CCNB1 induces a protein,nuclear factor 90(NF90),to bind and prolong the half-life of tight junction protein 1(TJP1)m RNA.Upregulation of TJP1 enhances tight junctions between cancer cells and inhibits NPC cell migration and invasion.This study reveals a novel biological function of circ CCNB1 in the migration and invasion of NPC by enhancing the tight junctions of cancer cells by binding to NF90 proteins and TJP1 m RNA,and may provide a potential therapeutic target for NPC.