Tea polyphenols inhibit the growth and angiogenesis of breast cancer xenografts in a mouse model

Objective:To investigate the anti-angiogenic effect of tea polyphenols(TPS)on breast cancer and normal tissues in a mouse model.Methods:Breast cancer was successfully implanted into 48 BALB/c mice,which were then randomly divided into a TP oral gavage group,a TP local injection group,a ginsenoside Rg3 group,and a model control group according to a random number table.The tumor inhibitory rates of each group were calculated,while microvessel density(MVD)and the expression of vascular endothelial growth factor(VEGF),basic fibroblast growth factor(bFGF),and tissue inhibitor of metalloproteinase(TIMP-2)were detected by immunohistochemistry.Results:TPs could inhibit the growth of breast cancer xenografts in the mouse model.The tumor inhibition rates of the TP oral gavage and TP local injection groups were 37.43%and 40.94%,respectively.Compared with the model control group,MVD and VEGF and bFGF expression was downregulated(all P<.05),whereas TIMP-2 expression was elevated in the TP oral gavage and TP local injection groups(P=.015 and P=.032).TPs showed no significant effect on MVD and VEGF and TIMP-2 expression in the heart,brain,and kidney of the mouse model.Conclusion:TPs can restrict the growth of breast cancer by specifically inhibiting the angiogenesis of breast tumor tissue while having little effect on the normal tissue of important organs including the heart,brain,and kidney.

Modified Liangfu granule exhibits anti-cancer effects in gastric cancer by regulating apoptosis-related proteins and genes

Objective:To investigate the anti-cancer effects and mechanism of modified Liangfu granule(MLFG)in BGC-823 gastric cancer cells.Methods:The drug serum was extracted from abdominal aorta of Wistar rats treated by cyclophosphamide,dioscin,MLFG(high-,medium-,and low-dose),respectively.MTS assay was performed to detect the effect of different concentrations of MLFG and dioscin on cell growth.The effect of MLFG on cellular apoptosis was detected by flow cytometry.Western blot was used to examine Bcl-2 and Akt in BGC-823 cells treated with MLFG.Quantitative real-time polymerase chain reaction assay was performed to determine the expression level of caspase-3,E2F1 and E2F3 genes in cells treated by MLFG-and dioscin-containing serum.Results:MLFG-and dioscin-containing serum inhibited the cell proliferation of BGC-823 cells.MLFG induced gastric carcinoma cell apoptosis and inhibited the expression of Bcl-2 and Akt in a dosedependent manner.MLFG also significantly induced the gene expression of caspase-3 and downregulated E2F1 and E2F3 gene expression.Conclusion:The effect of MLFG and dioscin on inhibiting cell proliferation and induction of apoptosis of gastric cancer cells might be related to the regulation of Bcl-2 and Akt proteins and the expression of caspase-3,E2F1 and E2F3 genes.