Analysis and Verification of the Conserved MYB Binding Element in the DFR Promoter in Compositae

Anthocyanins,ubiquitous in the Compositae family,are regulated by MYB(v-myb avian myeloblastosis viral oncogene homolog),playing an important role in anthocyanin synthesis.In this study,we analyzed the regulation pathway in which the MYB protein of subgroup 6 promotes dihydroflavonol reductase(DFR)expression in Compositae,and validated this law in Saussurea medusa through yeast one-hybrid experiments.Our results showed that MYB and DFR underwent purification selection,DFR promoter analysis revealed the presence of MYB binding site(GAGTTGAATGG)and bHLH binding site(CANNTG)at the sense strand of 84–116 nucleotide residues from the start codon.These two motifs were separated by 9–10 nucleotide residues,as existed in the DFR promoters of many Compositae plants.Furthermore,the yeast one-hybrid experiment demonstrated that SmMYB1 can activate the promoter of SmDFR.Our results provide a reference for further functional study of DFR in Compositae.

Thin Layer Identification of Jiedu Shengxue Granules and Determination of Notoginsenoside R1 Content

[Objectives]To establish the quality standard of hospital preparation Jiedu Shengxue granules.[Methods]Scleromitrion diffusum and Prunella vulgaris in Jiedu Shengxue granules were qualitatively identified by thin layer chromatography(TLC).A high performance liquid chromatography(HPLC)was established to determine the content of notoginsenoside R1 in the granule.[Results]The traditional Chinese medicinal materials in Jiedu Shengxue granules could be identified by TLC,and the characteristic spots were stable and clear.Notoginsenoside R1 had a good linear relationship in the range of 10.45-104.5μg/mL,with an average recovery of 98.52%and RSD=2.36%.[Conclusions]TLC and HPLC,as the quality control methods of Jiedu Shengxue granules,have high accuracy and good repeatability,which lays a foundation for the quality control of this mixture.

Comparative Transcriptome Analysis Reveals Different Mechanisms of Adaptation to Environment among Three Species of Saussurea DC.

Saussurea medusa,Saussurea hypsipeta and Saussurea obvallata are typical alpine snowline plants growing in the Qinghai-Tibet plateau.They are characterized by a specialized morphology.S.medusa and S.hypsipeta have very dense trichomes on whole plant,whereas S.obvallata has transparent bracts covered inflorescences.The different forms reflect their adaptation to cold environments.To investigate the different mechanisms of adaptation of these species to cold temperatures,transcriptome sequencing was performed in three species of Saussurea DC.A total of 116394137237 and 113879 Unigenes were identified from S.medusa,S.hypsipeta and S.obvallata,respectively.Of these,55909(48.03%),65519(47.74%)and 51889(45.56%)Unigenes were matched in public databases.GO analysis identified that most of annotated Unigenes in the three species of plants were related to cellular,metabolic,and single−organism processes,and binding and catalytic activities.The differential expression of 37 genes related to environmental adaptation were discovered by pairwise comparisons.Of these,two candidate genes(Interaptin-like and CSLB3)related to trichome development were identified only in S.medusa and S.hypsipeta,which was consistent with their distinct morphology.Our data can provide a valuable resource for the further studies on the adaptive mechanisms of molecular and functional ecology in Saussurea DC.