For a long time, ripened cheeses have been officially recognized as having human health beneficial properties. However, to date only a few studies have investigated the complex microbiota of S?o Jorge and Parmigiano-R...For a long time, ripened cheeses have been officially recognized as having human health beneficial properties. However, to date only a few studies have investigated the complex microbiota of S?o Jorge and Parmigiano-Reggiano cheeses in order to identify microorganisms with probiotic potential. Hence, with the purpose of analyzing the probiotic potential of autochthone bacteria from S?o Jorge and Parmigiano-Reggiano cheeses, lactic acid bacteria isolated from both products were isolated and their direct biotic interaction against three bacteria found in the human intestine evaluated. From a total of 225 bacterial autochthone isolates, 67 had synergetic behavior with Bifidobacterium animalis , 34 had no synergetic behavior with Shigella dysenteriae and 33 had antagonistic activity with this human pathogen. Biochemical tests were used to identify these cheese isolates with probiotic potential. Lactobacillus paracasei subsp paracasei Lactobacillus arhamnosus were found in São Jorge cheese and Lactobacillus rhamnosus , Lactobacillus buchneri and Lactobacillus curvatus in the Parmigiano cheese. Overall, initial exploratory studies with São Jorge cheese seem to show a higher number of potential probiotic agents than those found in the Parmigiano-Reggiano cheese. However, in order to better understand the functional potential of this traditional Portuguese cheese, further confirmatory studies should be pursued.展开更多
During 2016, following the implementation of a dioxin contamination monitoring in poultry meat (ducks) in a slaughterhouse, a positive sample was found. The investigation identified the wood shavings used as bedding m...During 2016, following the implementation of a dioxin contamination monitoring in poultry meat (ducks) in a slaughterhouse, a positive sample was found. The investigation identified the wood shavings used as bedding material in the duck farm as the possible source of contamination of these animals. In this episode, contamination profiles of higher and lower concentrations, seem to be very similar, being OCDD, OCDF and 1,2,3,4,6,7,8-HpCDD responsible for 74.4% of the total contamination of those animals. The study revealed a reduction of poultry contamination after the removal of the source of contamination, with an average daily depletion percentage level ranging between 1.43% and 4.35%.展开更多
This work describes the treatment of Pinus pinaster wood with four different industrial wood preservatives (two anti-bluing or fungicide and two fungicide/pesticide) and the detection and quantification of the dioxin ...This work describes the treatment of Pinus pinaster wood with four different industrial wood preservatives (two anti-bluing or fungicide and two fungicide/pesticide) and the detection and quantification of the dioxin contamination profile in the wood shavings. The samples were collected from poultry liters during three contamination incidents of poultry meat. Two methods used were, both nonpressure: one by immersing the wood samples in the preservative solution and the other by impregnation of the preservative solution into the wood, with vacuum. It was concluded that there is no difference in terms of contamination profile, caused by the different industrial wood treatment preservative products in study. A clear correlation between the commercial products used in wood treatment and the contamination profile of wood shavings that have been used as bedding material in poultry production was detected.展开更多
During 2006 and 2011, following the implementation of dioxin contamination monitoring in poultry meat, levels higher than legally allowed in meat from poultry slaughtered for human consumption, were found. The wood sh...During 2006 and 2011, following the implementation of dioxin contamination monitoring in poultry meat, levels higher than legally allowed in meat from poultry slaughtered for human consumption, were found. The wood shavings used as bedding material in the poultry farm showed considerable high contaminations, indicating that these materials were the likely source of contamination of the animals. Wood shavings samples (n = 23), used as poultry litters in intensive farms of broilers, were analysed. In both episodes, contamination profiles of higher and lower concentrations, seem to be very similar, being OCDD, OCDF, 1,2,3,4,6,7,8-HpCDD and 1,2,3,4,6,7,8-HpCDF responsible for 97.4 % of the total contamination. The present work describes the analytical adapted, used methodology and the specific clean-up procedures, which revealed that recoveries of <sup>13</sup>C<sub>12</sub>-Labelled compounds added to the wood shavings samples ranged from 71.3% to 86.3%.展开更多
文摘For a long time, ripened cheeses have been officially recognized as having human health beneficial properties. However, to date only a few studies have investigated the complex microbiota of S?o Jorge and Parmigiano-Reggiano cheeses in order to identify microorganisms with probiotic potential. Hence, with the purpose of analyzing the probiotic potential of autochthone bacteria from S?o Jorge and Parmigiano-Reggiano cheeses, lactic acid bacteria isolated from both products were isolated and their direct biotic interaction against three bacteria found in the human intestine evaluated. From a total of 225 bacterial autochthone isolates, 67 had synergetic behavior with Bifidobacterium animalis , 34 had no synergetic behavior with Shigella dysenteriae and 33 had antagonistic activity with this human pathogen. Biochemical tests were used to identify these cheese isolates with probiotic potential. Lactobacillus paracasei subsp paracasei Lactobacillus arhamnosus were found in São Jorge cheese and Lactobacillus rhamnosus , Lactobacillus buchneri and Lactobacillus curvatus in the Parmigiano cheese. Overall, initial exploratory studies with São Jorge cheese seem to show a higher number of potential probiotic agents than those found in the Parmigiano-Reggiano cheese. However, in order to better understand the functional potential of this traditional Portuguese cheese, further confirmatory studies should be pursued.
文摘During 2016, following the implementation of a dioxin contamination monitoring in poultry meat (ducks) in a slaughterhouse, a positive sample was found. The investigation identified the wood shavings used as bedding material in the duck farm as the possible source of contamination of these animals. In this episode, contamination profiles of higher and lower concentrations, seem to be very similar, being OCDD, OCDF and 1,2,3,4,6,7,8-HpCDD responsible for 74.4% of the total contamination of those animals. The study revealed a reduction of poultry contamination after the removal of the source of contamination, with an average daily depletion percentage level ranging between 1.43% and 4.35%.
文摘This work describes the treatment of Pinus pinaster wood with four different industrial wood preservatives (two anti-bluing or fungicide and two fungicide/pesticide) and the detection and quantification of the dioxin contamination profile in the wood shavings. The samples were collected from poultry liters during three contamination incidents of poultry meat. Two methods used were, both nonpressure: one by immersing the wood samples in the preservative solution and the other by impregnation of the preservative solution into the wood, with vacuum. It was concluded that there is no difference in terms of contamination profile, caused by the different industrial wood treatment preservative products in study. A clear correlation between the commercial products used in wood treatment and the contamination profile of wood shavings that have been used as bedding material in poultry production was detected.
文摘During 2006 and 2011, following the implementation of dioxin contamination monitoring in poultry meat, levels higher than legally allowed in meat from poultry slaughtered for human consumption, were found. The wood shavings used as bedding material in the poultry farm showed considerable high contaminations, indicating that these materials were the likely source of contamination of the animals. Wood shavings samples (n = 23), used as poultry litters in intensive farms of broilers, were analysed. In both episodes, contamination profiles of higher and lower concentrations, seem to be very similar, being OCDD, OCDF, 1,2,3,4,6,7,8-HpCDD and 1,2,3,4,6,7,8-HpCDF responsible for 97.4 % of the total contamination. The present work describes the analytical adapted, used methodology and the specific clean-up procedures, which revealed that recoveries of <sup>13</sup>C<sub>12</sub>-Labelled compounds added to the wood shavings samples ranged from 71.3% to 86.3%.
