Natural products are the prominent sources of drugs to combat various infectious diseases.The rapid progress of multi-resistance and pan-resistant pathogens to various medicines represents today a major challenge.The ...Natural products are the prominent sources of drugs to combat various infectious diseases.The rapid progress of multi-resistance and pan-resistant pathogens to various medicines represents today a major challenge.The search for novel natural products requires a quick and efficient approach to distinguish novel compounds from the known ones,a process called dereplication.Dereplication strategy is generic and time-saving,avoids isolation/purification of known compounds,enables an efficient liquid chromatography peak annotation of most of the studied compounds and can be well adapted for plant chemotaxonomy,phytochemical screening and metabolite profiling.In this review,recent developments of hyphenated techniques towards dereplication of active compounds,chemotaxonomy,metabolite profiling and rapid detection of novel compounds in medicinal plant extracts are presented.展开更多
Objective:To isolate and characterize chemical constituents of the roots of Zanthoxylumchalybeum(Z.chalybeum).Methods:A number of phytochemical tests were applied to identify the class of compounds present in the CH_(...Objective:To isolate and characterize chemical constituents of the roots of Zanthoxylumchalybeum(Z.chalybeum).Methods:A number of phytochemical tests were applied to identify the class of compounds present in the CH_(2)Cl_(2)/CH_(3)OH(1:1)root extract.Column chromatographic separation technique was applied to separate the constituents of the CH_(2)Cl_(2)/CH_(3)OH(1:1)root extract and various spectroscopic techniques[UV-vis,infrared radiation,nuclear magnetic resonance(NMR)(^(1)H-NMR,^(13)C NMR,DEPT-135,COSY,gHSQC and gHMBC)]were used to determine the structures of pure compounds.Results:Phytochemical screening of the CH_(2)Cl_(2)/CH_(3)OH(1:1)root extract of Z.chalybeum revealed the presence of alkaloids,flavonoids,terpenoids,tannins and anthraquinones.Column chromatographic separation of the extract yielded a new coniferyl alcohol derivative,2,3-epoxy-6,7-methylenedioxyconiferyl alcohol(1)together with the known alkaloid,dihydrochelerythrine(2).Conclusions:The present work conducted on the CH2Cl2:CH3OH(1:1)root extract of Z.chalybeum identified various class of compounds present in the root extract.Complete characterization of two compounds were done using spectroscopic techniques of which a coniferyl alcohol derivative(1)was identified for the first time.展开更多
文摘Natural products are the prominent sources of drugs to combat various infectious diseases.The rapid progress of multi-resistance and pan-resistant pathogens to various medicines represents today a major challenge.The search for novel natural products requires a quick and efficient approach to distinguish novel compounds from the known ones,a process called dereplication.Dereplication strategy is generic and time-saving,avoids isolation/purification of known compounds,enables an efficient liquid chromatography peak annotation of most of the studied compounds and can be well adapted for plant chemotaxonomy,phytochemical screening and metabolite profiling.In this review,recent developments of hyphenated techniques towards dereplication of active compounds,chemotaxonomy,metabolite profiling and rapid detection of novel compounds in medicinal plant extracts are presented.
文摘Objective:To isolate and characterize chemical constituents of the roots of Zanthoxylumchalybeum(Z.chalybeum).Methods:A number of phytochemical tests were applied to identify the class of compounds present in the CH_(2)Cl_(2)/CH_(3)OH(1:1)root extract.Column chromatographic separation technique was applied to separate the constituents of the CH_(2)Cl_(2)/CH_(3)OH(1:1)root extract and various spectroscopic techniques[UV-vis,infrared radiation,nuclear magnetic resonance(NMR)(^(1)H-NMR,^(13)C NMR,DEPT-135,COSY,gHSQC and gHMBC)]were used to determine the structures of pure compounds.Results:Phytochemical screening of the CH_(2)Cl_(2)/CH_(3)OH(1:1)root extract of Z.chalybeum revealed the presence of alkaloids,flavonoids,terpenoids,tannins and anthraquinones.Column chromatographic separation of the extract yielded a new coniferyl alcohol derivative,2,3-epoxy-6,7-methylenedioxyconiferyl alcohol(1)together with the known alkaloid,dihydrochelerythrine(2).Conclusions:The present work conducted on the CH2Cl2:CH3OH(1:1)root extract of Z.chalybeum identified various class of compounds present in the root extract.Complete characterization of two compounds were done using spectroscopic techniques of which a coniferyl alcohol derivative(1)was identified for the first time.
