The detection and analysis of rare cells in complex media such as blood is increasingly important in biomedical research and clinical diagnostics.Micro-Hall detectors(μHD)for magnetic detection in blood have previous...The detection and analysis of rare cells in complex media such as blood is increasingly important in biomedical research and clinical diagnostics.Micro-Hall detectors(μHD)for magnetic detection in blood have previously demonstrated ultrahigh sensitivity to rare cells.This sensitivity originates from the minimal magnetic background in blood,obviating cumbersome and detrimental sample preparation.However,the translation of this technology to clinical applications has been limited by inherently low throughput(<1 mL/h),susceptibility to clogging,and incompatibility with commercial CMOS foundry processing.To help overcome these challenges,we have developed CMOS-compatible graphene Hall sensors for integration with PDMS microfluidics for magnetic sensing in blood.We demonstrate that these grapheneμHDs can match the performance of the best publishedμHDs,can be passivated for robust use with whole blood,and can be integrated with microfluidics and sensing electronics for in-flow detection of magnetic beads.We show a proof-of-concept validation of our system on a silicon substrate and detect magnetic agarose beads,as a model for cells,demonstrating promise for future integration in clinical applications with a custom CMOS chip.展开更多
The sense of touch is critical to dexterous use of the hands and thus an essential component of efforts to restore hand function after amputation or paralysis.Prosthetic systems have addressed this goal with wearable ...The sense of touch is critical to dexterous use of the hands and thus an essential component of efforts to restore hand function after amputation or paralysis.Prosthetic systems have addressed this goal with wearable tactile sensors.However,such wearable sensors are suboptimal for neuroprosthetic systems designed to reanimate a patient’s own paralyzed hand.Here,we developed an implantable tactile sensing system intended for subdermal placement.The system is composed of a microfabricated capacitive pressure sensor,a custom integrated circuit supporting wireless powering and data transmission,and a laser-fused hermetic silica package.The miniature device was validated through simulations,benchtop assessment,and testing in a primate hand.The sensor implanted in the fingertip accurately measured applied skin forces with a resolution of 4.3 mN.The output from this novel sensor could be encoded in the brain with microstimulation to provide tactile feedback.More broadly,the materials,system design,and fabrication approach establish new foundational capabilities for various applications of implantable sensing systems.展开更多
基金This work was carried out in part at the Singh Center for Nanotechnology,which is supported by the NSF National Nanotechnology Coordinated Infrastructure Program under grant NNCI-2025608The authors would like to acknowledge support from National Institute of Allergy and Infectious Diseases(NIAID),R61AI147406from the National Cancer Institute(NCI),R33CA206907.We thank Chengyu Wen for help with the graphene mobility measurements.
文摘The detection and analysis of rare cells in complex media such as blood is increasingly important in biomedical research and clinical diagnostics.Micro-Hall detectors(μHD)for magnetic detection in blood have previously demonstrated ultrahigh sensitivity to rare cells.This sensitivity originates from the minimal magnetic background in blood,obviating cumbersome and detrimental sample preparation.However,the translation of this technology to clinical applications has been limited by inherently low throughput(<1 mL/h),susceptibility to clogging,and incompatibility with commercial CMOS foundry processing.To help overcome these challenges,we have developed CMOS-compatible graphene Hall sensors for integration with PDMS microfluidics for magnetic sensing in blood.We demonstrate that these grapheneμHDs can match the performance of the best publishedμHDs,can be passivated for robust use with whole blood,and can be integrated with microfluidics and sensing electronics for in-flow detection of magnetic beads.We show a proof-of-concept validation of our system on a silicon substrate and detect magnetic agarose beads,as a model for cells,demonstrating promise for future integration in clinical applications with a custom CMOS chip.
基金supported by National Institutes of Health grant R01NS107550supported by the NSF National Nanotechnology Coordinated Infrastructure Program under grant NNCI-1542153.
文摘The sense of touch is critical to dexterous use of the hands and thus an essential component of efforts to restore hand function after amputation or paralysis.Prosthetic systems have addressed this goal with wearable tactile sensors.However,such wearable sensors are suboptimal for neuroprosthetic systems designed to reanimate a patient’s own paralyzed hand.Here,we developed an implantable tactile sensing system intended for subdermal placement.The system is composed of a microfabricated capacitive pressure sensor,a custom integrated circuit supporting wireless powering and data transmission,and a laser-fused hermetic silica package.The miniature device was validated through simulations,benchtop assessment,and testing in a primate hand.The sensor implanted in the fingertip accurately measured applied skin forces with a resolution of 4.3 mN.The output from this novel sensor could be encoded in the brain with microstimulation to provide tactile feedback.More broadly,the materials,system design,and fabrication approach establish new foundational capabilities for various applications of implantable sensing systems.
