Aim: To study the changes in blood of human neutrophil lipocalin (HNL) and C-reactive protein (CRP) during the course of an acute infection in children. Methods: Children (n = 92) hospitalized with symptoms and signs ...Aim: To study the changes in blood of human neutrophil lipocalin (HNL) and C-reactive protein (CRP) during the course of an acute infection in children. Methods: Children (n = 92) hospitalized with symptoms and signs of acute infections were included and categorized into five groups, i.e. bacterial infection, suspected bacterial infection, viral infection, suspected viral infection and others. Blood was taken at admittance and the following 3-4 d for the measurement of CRP and HNL . Results: Both CRP and HNL were significantly raised at admittance in bacterial infection as compared to viral infection (p < 0.001). After 25-48 h, 83%of the children with bacterial infections still had raised CRP levels in contrast to 11%having raised HNL levels. The levels of CRP, but not those of HNL, were significantly correlated to days of symptoms before admission. Conclusions: HNL is a promising diagnostic tool in the distinction of acute infections caused by bacteria or virus. The differences in the kinetics of CRP and HNL make HNL a bettermarker for monitoring antibacterial treatment, since HNL is probably elevated only when an active bacterial infection is at hand.展开更多
Background: The expression of CD64 (Fcγ RI) is increased from an almost negligible to a marked level on neutrophils in patients with bacterial infections. CD64 expression on neutrophils might therefore be a potential...Background: The expression of CD64 (Fcγ RI) is increased from an almost negligible to a marked level on neutrophils in patients with bacterial infections. CD64 expression on neutrophils might therefore be a potential candidate for the diagnosis of bacterial infections in infants. Aim: This study was performed to monitor changes of neutrophil expression of CD64 during the postpartum period to further evaluate the usefulness of this analysis. The possible influence on the expression of this receptor by other factors was also investigated, including respiratory distress syndrome (RDS) and preterm rupture of the membranes (PROM). Methods: Cell surface expression of CD64 on neutrophils from preterm and term newborn infants and healthy adults was analysed by flow cytometry. The expression of the other Fcγ receptors, CD32 and CD16, and the complement receptors CD11b/CD18 and CD35 was also analysed for comparison. Results: Neutrophils from preterm newborn infants showed a moderately increased level of CD64 expression that, during their first month of life, was reduced to the level observed on neutrophils from term newborn infants and adults. In contrast, the level of neutrophil expression of CD32 and CD16 was significantly lower in preterm than term newborn infants and adults. Neutrophils from all groups indicated similar levels of CD11b expression, but the expression on neutrophils from newborn infants increased after birth. Conclusion: Our results showed that neutrophil expression of CD64 is moderately increased in preterm newborn infants at birth. It seems not to be influenced by RDS, PROM or other factors related to preterm birth but by bacterial infection.展开更多
文摘Aim: To study the changes in blood of human neutrophil lipocalin (HNL) and C-reactive protein (CRP) during the course of an acute infection in children. Methods: Children (n = 92) hospitalized with symptoms and signs of acute infections were included and categorized into five groups, i.e. bacterial infection, suspected bacterial infection, viral infection, suspected viral infection and others. Blood was taken at admittance and the following 3-4 d for the measurement of CRP and HNL . Results: Both CRP and HNL were significantly raised at admittance in bacterial infection as compared to viral infection (p < 0.001). After 25-48 h, 83%of the children with bacterial infections still had raised CRP levels in contrast to 11%having raised HNL levels. The levels of CRP, but not those of HNL, were significantly correlated to days of symptoms before admission. Conclusions: HNL is a promising diagnostic tool in the distinction of acute infections caused by bacteria or virus. The differences in the kinetics of CRP and HNL make HNL a bettermarker for monitoring antibacterial treatment, since HNL is probably elevated only when an active bacterial infection is at hand.
文摘Background: The expression of CD64 (Fcγ RI) is increased from an almost negligible to a marked level on neutrophils in patients with bacterial infections. CD64 expression on neutrophils might therefore be a potential candidate for the diagnosis of bacterial infections in infants. Aim: This study was performed to monitor changes of neutrophil expression of CD64 during the postpartum period to further evaluate the usefulness of this analysis. The possible influence on the expression of this receptor by other factors was also investigated, including respiratory distress syndrome (RDS) and preterm rupture of the membranes (PROM). Methods: Cell surface expression of CD64 on neutrophils from preterm and term newborn infants and healthy adults was analysed by flow cytometry. The expression of the other Fcγ receptors, CD32 and CD16, and the complement receptors CD11b/CD18 and CD35 was also analysed for comparison. Results: Neutrophils from preterm newborn infants showed a moderately increased level of CD64 expression that, during their first month of life, was reduced to the level observed on neutrophils from term newborn infants and adults. In contrast, the level of neutrophil expression of CD32 and CD16 was significantly lower in preterm than term newborn infants and adults. Neutrophils from all groups indicated similar levels of CD11b expression, but the expression on neutrophils from newborn infants increased after birth. Conclusion: Our results showed that neutrophil expression of CD64 is moderately increased in preterm newborn infants at birth. It seems not to be influenced by RDS, PROM or other factors related to preterm birth but by bacterial infection.
