A primary cDNA library of Aspergillus niger H1 was constructed using the switching mechanism at the 5′ end of the RNA transcript（SMART） technique. A total of 169 clones exhibited halos when grown on tricalcium phos...A primary cDNA library of Aspergillus niger H1 was constructed using the switching mechanism at the 5′ end of the RNA transcript（SMART） technique. A total of 169 clones exhibited halos when grown on tricalcium phosphate medium, and the H-46 clone displayed a clear halo. The full-length c DNA of the clone H-46 clone was 1 407 bp in length with a complete open reading frame（ORF） of 816 bp, and it encoded a protein that contained 272 amino acids. Multiple alignment analysis revealed a high degree of homology between the ORFs of the H-46 clone and the Bax inhibitor family（BI-1-like） proteins of other fungi. Acetic acid was secreted by Escherichia coli DH5α that express the BI-1-like gene. The level attained was 492.52 mg L^（-1）, which was associated with the release of 0.212 mg m L^（-1） of soluble phosphate at 28 h. These results showed that the heterologous expression of BI-1-like genes in Eschericha coli DH5α increased the secretion of acetic acid by altering the membrane permeability and enhancing the solubility of phosphate（P）.展开更多
基金supported by the National Natural Science Foundation of China(41440008)
文摘A primary cDNA library of Aspergillus niger H1 was constructed using the switching mechanism at the 5′ end of the RNA transcript（SMART） technique. A total of 169 clones exhibited halos when grown on tricalcium phosphate medium, and the H-46 clone displayed a clear halo. The full-length c DNA of the clone H-46 clone was 1 407 bp in length with a complete open reading frame（ORF） of 816 bp, and it encoded a protein that contained 272 amino acids. Multiple alignment analysis revealed a high degree of homology between the ORFs of the H-46 clone and the Bax inhibitor family（BI-1-like） proteins of other fungi. Acetic acid was secreted by Escherichia coli DH5α that express the BI-1-like gene. The level attained was 492.52 mg L^（-1）, which was associated with the release of 0.212 mg m L^（-1） of soluble phosphate at 28 h. These results showed that the heterologous expression of BI-1-like genes in Eschericha coli DH5α increased the secretion of acetic acid by altering the membrane permeability and enhancing the solubility of phosphate（P）.
