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马铃薯半胱氨酸蛋白酶抑制剂基因(PCPI)在马铃薯晚疫病抗性中的功能表征研究 被引量:1
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作者 史伟 韦献雅 +2 位作者 付绍宏 阳翠 陈志国 《种子》 北大核心 2019年第11期108-112,共5页
由于马铃薯半胱氨酸蛋白酶抑制剂基因(PCPI)构造载体双链RNA在疫霉抗性马铃薯品种中薯5号中瞬时表达,PCPI转录减少了93.94%。PCPI的沉默导致了病变尺寸和水浸的显著增长,表明半胱氨酸蛋白酶抑制剂能在限制病变扩张中发挥作用。本研究通... 由于马铃薯半胱氨酸蛋白酶抑制剂基因(PCPI)构造载体双链RNA在疫霉抗性马铃薯品种中薯5号中瞬时表达,PCPI转录减少了93.94%。PCPI的沉默导致了病变尺寸和水浸的显著增长,表明半胱氨酸蛋白酶抑制剂能在限制病变扩张中发挥作用。本研究通过农杆菌渗入法生产了针对PCPI用于测试在马铃薯中获得瞬态基因沉默可能性的dsRNA构造,结果显示:在马铃薯植株叶片上的构造导致PCPI的下调和相应转录积累的降低;沉默的马铃薯基因编码了马铃薯晚疫病的半胱氨酸蛋白酶抑制剂,用以抵抗中薯5号品种破裂的阻力。该研究可用于生产稳定的敏感基因型的抗性转基因植物。 展开更多
关键词 马铃薯 RNA干扰 晚疫病 农杆菌渗入法 半胱氨酸蛋白酶抑制剂
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高温大曲发酵过程中微生物多样性研究 被引量:7
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作者 付绍鸿 赵荣寿 +1 位作者 杨柳 周蔺 《酿酒科技》 2019年第3期76-79,共4页
利用高通量测序技术对在发酵和储存过程中的高温大曲微生物的多样性进行研究分析。经研究发现:大曲内细菌的多样性呈波浪形变化,在门、纲、目、科、属级水平上,厚壁菌门、芽孢杆菌纲、芽孢杆菌目、芽孢杆菌科、高温放线菌科、乳杆菌科... 利用高通量测序技术对在发酵和储存过程中的高温大曲微生物的多样性进行研究分析。经研究发现:大曲内细菌的多样性呈波浪形变化,在门、纲、目、科、属级水平上,厚壁菌门、芽孢杆菌纲、芽孢杆菌目、芽孢杆菌科、高温放线菌科、乳杆菌科、明串珠菌科、乳杆菌属和魏斯氏菌属占优势。 展开更多
关键词 高温大曲 高通量测序 微生物 多样性
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Labor Transfer:A Catalyst or Anticatalyst for Poverty Alleviation?--A study based on a survey of 237 rural households in Yi communities in Liangshan
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作者 Zhang Lei fu shaohong Wu Lingwei(翻译) 《Contemporary Social Sciences》 2020年第6期42-60,共19页
This paper takes income and“two no worries and three guarantees”(rural poor people have no worry about food and clothing and have access to compulsory education,basic medical services,and safe housing)as two essenti... This paper takes income and“two no worries and three guarantees”(rural poor people have no worry about food and clothing and have access to compulsory education,basic medical services,and safe housing)as two essential indexes,and is based on data collected from a survey of 237 rural households living below the poverty level in Yi communities in Liangshan.Through case studies and positive analysis,we explored the impact of labor transfer on poverty alleviation.We found that the following factors contribute to a double paradox of labor service-enabled poverty alleviation:An income growth trend which prioritizes“external income”(from urban work)over“internal income”(from an agricultural business),restrictions by performance indexes,as well as a lack of public services in rural areas.Although labor transfer can help rural households increase household income and non-agricultural income and reduce poverty within a short period of time,it can hardly promote agricultural businesses through non-agricultural employment and thereby help poverty-stricken rural households permanently alleviate poverty.Although labor transfer can exacerbate various issues facing poverty-stricken households(such as a delay in housing construction,school drop-out rates and health risks for the elderly),it can also play a positive role in raising the profile of farmers and improving rural education and living environments.Based on this study,we propose continuing the supply-side reform in the labor market,implementing differentiated poverty alleviation policies,improving the existing mechanism for poverty alleviation assessment,establishing rural public services,and launching a corresponding multi-channel financing mechanism. 展开更多
关键词 labor transfer targeted poverty alleviation Yi communities “two no worries and three guarantees”
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半潜船装卸大型结构物的横向滑移装船操作及风险防控
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作者 付绍洪 刘志恒 袁梦 《航海技术》 2023年第4期12-16,共5页
大型结构物具有体积大、质量重的特点,万吨级以上的大型结构物一般采用滑移装船的方式,装船操作过程中受到许多条件限制,需要提前进行策划和评估风险。以半潜式平台船体装船操作为典型案例,综合考虑码头水深、潮汐影响、滑装设备、靠泊... 大型结构物具有体积大、质量重的特点,万吨级以上的大型结构物一般采用滑移装船的方式,装船操作过程中受到许多条件限制,需要提前进行策划和评估风险。以半潜式平台船体装船操作为典型案例,综合考虑码头水深、潮汐影响、滑装设备、靠泊方案、滑移操作、压排水操作、监测和通信等因素,分析大型结构物横向滑移装船的操作风险和应对方案,总结关键操作和风险管理的经验,为同类项目的装船操作提供参考。 展开更多
关键词 半潜船 大型结构物 滑移装船 系泊 压排水 风险防控
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Characteristics of Cd isotopic compositions and their genetic significance in the lead-zinc deposits of SW China 被引量:18
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作者 ZHU ChuanWei WEN HanJie +4 位作者 ZHANG YuXu FAN HaiFeng fu shaohong XU Juan QIN TingRong 《Science China Earth Sciences》 SCIE EI CAS 2013年第12期2056-2065,共10页
Up to now,the evaporation and condensation,as well as the biological absorption and inorganic absorptions,have been proved to be major factors in Cd isotope fractionation.And Cd isotopes have been widely applied in st... Up to now,the evaporation and condensation,as well as the biological absorption and inorganic absorptions,have been proved to be major factors in Cd isotope fractionation.And Cd isotopes have been widely applied in studies on the universal evolution and marine environment and so on.However,only a few researches have been conducted in applying Cd isotopes to trace the source of metallogenic material and the evolution of the ore-forming fluid in a complex mineralization environment,especially in a hydrothermal ore-formation system.We measured the Cd isotopic compositions of sphalerite,galena,and ores from five lead-zinc deposits in SW China,and found that the 114/110Cd values varied from 1.53‰to 0.34‰,with a total range of1.87‰,which is greater than most of measured geological samples.Meanwhile,through contrasting the Cd content with Cd isotopic compositions of different deposits,it may be concluded that different genetic lead-zinc deposits have different Cd content and isotopic compositions,which could be a tool for the studies on the origin of ore deposits.Also,the biomineralization and crystal fractionation may also result in Cd isotope fractionation.In a word,although the research of Cd isotopes is presently at the preliminary stage(especially in hydrothermal ore-formation system),this study demonstrated that Cd isotopes can give a clue in tracing the evolution of ore-forming fluid and metallogenic environment. 展开更多
关键词 中国西南地区 同位素组成 铅锌矿床 镉含量 成因意义 同位素分馏 特征 生物吸收
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