[Objectives] The purpose of this study is to dissociate endophytic fungus producing diterpenoids from Torreya fargesii tissue and examines its inhibiting effect on tumor cells. [Methods]Plant endophytes were isolated ...[Objectives] The purpose of this study is to dissociate endophytic fungus producing diterpenoids from Torreya fargesii tissue and examines its inhibiting effect on tumor cells. [Methods]Plant endophytes were isolated and purified to study their resistance to Gram-positive( G+) and Gram-negative bacteria( G-). High performance liquid chromatography( HPLC) was used for analysis of the retention time,relative peak area and percentage content of its metabolite. By liquid chromatography-mass spectrometry( HPLC-MS),the material characteristic of the ion pair information of the metabolites was measured. The bacterial strain was also classified. [Results] The results showed that the secondary metabolites produced by the strain BP6 T3 possessed double resistance to G+and G-bacteria. The strain was identified as Penicillium sp by preliminary classification. Through HPLC analysis,the retention time of fermentation extracts was 12. 8 min with almost the same as the standard of taxol. According to the chromatograph,the relative peak area was 12 887. 11,the average relative percentage was about 15. 8%,and the content of taxol analogs in fermentation broth reached 16. 59 mg/L. The material characteristic of the formation of ion fragments of taxane analogues in metabolic extracts was identical to that of the taxol standard determined by HPLC-MS. It can be initially determined that strain BP6 T3 can produce taxane compounds. Taxol substance produced by this strain had obvious inhibitory effect on Hela cells with the concentration increasing. Different precursors had a significant effect on the production of paclitaxel metabolites in this strain. L-phenylalanine was used as the precursor and the yield increased most,with an increase rate of 425. 7%. [Conclusions] The strain is expected to be used for mass production in antitumor drug taxol.展开更多
目的系统评价虚拟现实(VR)技术在全麻手术患儿术前焦虑中的应用效果,为临床规范有效地应用VR技术提供证据。方法检索PubMed、EMBASE、Cochrane Library、OVID(JBI)循证卫生保健数据库、Web of Science、CINAHL、中国生物医学文献数据库...目的系统评价虚拟现实(VR)技术在全麻手术患儿术前焦虑中的应用效果,为临床规范有效地应用VR技术提供证据。方法检索PubMed、EMBASE、Cochrane Library、OVID(JBI)循证卫生保健数据库、Web of Science、CINAHL、中国生物医学文献数据库、CNKI和万方数据库、维普数据库中应用VR技术对全麻手术患儿进行术前焦虑干预的随机对照试验和临床对照试验,采用Cochrane偏倚风险评估工具2.0对文献质量进行评价,采用RevMan 5.3软件进行Meta分析。结果共纳入12项研究,984例患者。Meta分析结果显示,与传统方法相比,VR技术的应用可显著降低患儿术前焦虑,尤其是麻醉诱导时的焦虑水平(MD=-0.79,95%CI:-0.98~-0.61,P<0.01),提高患儿麻醉诱导依从性(RR=0.46,95%CI:0.30~0.70,P<0.01),但对患儿苏醒期躁动水平无明显影响(MD=-0.14,95%CI:-0.64~0.36,P=0.28)。VR技术在是否能影响患儿苏醒期躁动发生率、术后行为改变、疼痛水平、生命体征、父母/监护人焦虑水平或满意度、麻醉镇痛药物应用等方面,目前尚无明确结论。结论应用VR技术可以有效减轻患儿术前焦虑,尤其是麻醉诱导时的焦虑水平,提升患儿麻醉诱导依从性,保障手术安全进行,是一种有效的辅助性干预手段。但其是否能减少患儿麻醉及镇痛药物需要、术后行为改变及苏醒期躁动发生,稳定患儿生命体征,降低患儿疼痛水平,减轻患儿父母/监护人焦虑,提升患儿父母/监护人满意度等仍无定论。展开更多
以我国实际电网为例,研究大型风电场对送端电网暂态稳定特性的影响规律。研究表明,由于风电机组本身不存在功角同步的问题,在送电通道功率不变的情况下,风电接入后系统的暂态功角稳定性有所改善,但导致系统的暂态电压稳定性变差。静止...以我国实际电网为例,研究大型风电场对送端电网暂态稳定特性的影响规律。研究表明,由于风电机组本身不存在功角同步的问题,在送电通道功率不变的情况下,风电接入后系统的暂态功角稳定性有所改善,但导致系统的暂态电压稳定性变差。静止无功补偿器(static var compensator,SVC)有利于提高送端电网的暂态功角稳定性和暂态电压稳定性。展开更多
基金Supported by Provincial College Students’Innovation and Entrepreneurship Training Program of Colleges and Universities in Hubei Province in 2017(2013)"Strategic Emerging(Pillar)Industrial Talent Training Program"of Colleges and Universities in Hubei Province[Hubei Provincial Department of Education EJiao Gao(201711798030)No.11]+2 种基金Pilot Funded Project of"Comprehensive Professional Reform"of Provincial Department of Education and Provincial Department of Finance[EJiao Gao Ban(2014)No.6]Hubei Educational Science"Twelfth Five-Year Plan"Project(2014B272)School Youth Natural Science Foundation(2013dhzk003)
文摘[Objectives] The purpose of this study is to dissociate endophytic fungus producing diterpenoids from Torreya fargesii tissue and examines its inhibiting effect on tumor cells. [Methods]Plant endophytes were isolated and purified to study their resistance to Gram-positive( G+) and Gram-negative bacteria( G-). High performance liquid chromatography( HPLC) was used for analysis of the retention time,relative peak area and percentage content of its metabolite. By liquid chromatography-mass spectrometry( HPLC-MS),the material characteristic of the ion pair information of the metabolites was measured. The bacterial strain was also classified. [Results] The results showed that the secondary metabolites produced by the strain BP6 T3 possessed double resistance to G+and G-bacteria. The strain was identified as Penicillium sp by preliminary classification. Through HPLC analysis,the retention time of fermentation extracts was 12. 8 min with almost the same as the standard of taxol. According to the chromatograph,the relative peak area was 12 887. 11,the average relative percentage was about 15. 8%,and the content of taxol analogs in fermentation broth reached 16. 59 mg/L. The material characteristic of the formation of ion fragments of taxane analogues in metabolic extracts was identical to that of the taxol standard determined by HPLC-MS. It can be initially determined that strain BP6 T3 can produce taxane compounds. Taxol substance produced by this strain had obvious inhibitory effect on Hela cells with the concentration increasing. Different precursors had a significant effect on the production of paclitaxel metabolites in this strain. L-phenylalanine was used as the precursor and the yield increased most,with an increase rate of 425. 7%. [Conclusions] The strain is expected to be used for mass production in antitumor drug taxol.
文摘目的系统评价虚拟现实(VR)技术在全麻手术患儿术前焦虑中的应用效果,为临床规范有效地应用VR技术提供证据。方法检索PubMed、EMBASE、Cochrane Library、OVID(JBI)循证卫生保健数据库、Web of Science、CINAHL、中国生物医学文献数据库、CNKI和万方数据库、维普数据库中应用VR技术对全麻手术患儿进行术前焦虑干预的随机对照试验和临床对照试验,采用Cochrane偏倚风险评估工具2.0对文献质量进行评价,采用RevMan 5.3软件进行Meta分析。结果共纳入12项研究,984例患者。Meta分析结果显示,与传统方法相比,VR技术的应用可显著降低患儿术前焦虑,尤其是麻醉诱导时的焦虑水平(MD=-0.79,95%CI:-0.98~-0.61,P<0.01),提高患儿麻醉诱导依从性(RR=0.46,95%CI:0.30~0.70,P<0.01),但对患儿苏醒期躁动水平无明显影响(MD=-0.14,95%CI:-0.64~0.36,P=0.28)。VR技术在是否能影响患儿苏醒期躁动发生率、术后行为改变、疼痛水平、生命体征、父母/监护人焦虑水平或满意度、麻醉镇痛药物应用等方面,目前尚无明确结论。结论应用VR技术可以有效减轻患儿术前焦虑,尤其是麻醉诱导时的焦虑水平,提升患儿麻醉诱导依从性,保障手术安全进行,是一种有效的辅助性干预手段。但其是否能减少患儿麻醉及镇痛药物需要、术后行为改变及苏醒期躁动发生,稳定患儿生命体征,降低患儿疼痛水平,减轻患儿父母/监护人焦虑,提升患儿父母/监护人满意度等仍无定论。
基金国家自然科学基金资助项目(51807149）青海多能互补基础优化研究(SGQHJY00GHJS1700197)+1 种基金Supported by the National Natural Science Foundation of China(51807149)Study on Optimization of Multi-energy Complementary in Qinghai(SGQHJY00GHJS1700197)
文摘以我国实际电网为例,研究大型风电场对送端电网暂态稳定特性的影响规律。研究表明,由于风电机组本身不存在功角同步的问题,在送电通道功率不变的情况下,风电接入后系统的暂态功角稳定性有所改善,但导致系统的暂态电压稳定性变差。静止无功补偿器(static var compensator,SVC)有利于提高送端电网的暂态功角稳定性和暂态电压稳定性。
