Plant male reproductive development is a complex biological process, but the underlying mechanism is not well understood. Here, we characterized a rice （Oryza sativa L.） male sterile mutant. Based on map- based clon...Plant male reproductive development is a complex biological process, but the underlying mechanism is not well understood. Here, we characterized a rice （Oryza sativa L.） male sterile mutant. Based on map- based cloning and sequence analysis, we identified a 1,459-bp deletion in an adenosine triphosphate （ATP）-binding cassette （ABC） transporter gene, OsABCG15, causing abnormal anthers and male sterility. Therefore, we named this mutant osabcgl5. Expression analysis showed that OsABCG15 is expressed specifically in developmental anthers from stage 8 （meiosis II stage） to stage 10 （late microspore stage）. Two genes CYP704B2 and WDA1, involved in the biosynthesis of very-long-chain fatty acids for the establishment of the anther cuticle and pollen exine, were downregulated in osabcgl5 mutant, suggesting that OsABCG15 may play a key function in the processes related to sporopollenin biosynthesis or sporopollenin transfer from tapetal cells to anther Iocules. Consistently, histological analysis showed that osabcgl5 mutants developed obvious abnormality in postmeiotic tapetum degeneration, leading to rapid degredation of young microspores. The results suggest that OsABCG15 plays a critical role in exine formation and pollen development, similar to the homologous gene of AtABCG26 in Arabidopsis. This work is helpful to understand the regulatory network in rice anther development.展开更多
基金supported by a grant from the National High Technology Research and Development Program of China (2012AA10A303)
文摘Plant male reproductive development is a complex biological process, but the underlying mechanism is not well understood. Here, we characterized a rice （Oryza sativa L.） male sterile mutant. Based on map- based cloning and sequence analysis, we identified a 1,459-bp deletion in an adenosine triphosphate （ATP）-binding cassette （ABC） transporter gene, OsABCG15, causing abnormal anthers and male sterility. Therefore, we named this mutant osabcgl5. Expression analysis showed that OsABCG15 is expressed specifically in developmental anthers from stage 8 （meiosis II stage） to stage 10 （late microspore stage）. Two genes CYP704B2 and WDA1, involved in the biosynthesis of very-long-chain fatty acids for the establishment of the anther cuticle and pollen exine, were downregulated in osabcgl5 mutant, suggesting that OsABCG15 may play a key function in the processes related to sporopollenin biosynthesis or sporopollenin transfer from tapetal cells to anther Iocules. Consistently, histological analysis showed that osabcgl5 mutants developed obvious abnormality in postmeiotic tapetum degeneration, leading to rapid degredation of young microspores. The results suggest that OsABCG15 plays a critical role in exine formation and pollen development, similar to the homologous gene of AtABCG26 in Arabidopsis. This work is helpful to understand the regulatory network in rice anther development.
