Changes of hs-CRP, S100B and NSE levels in serum and cerebrospinal fluid of children with epilepsy and their correlation with the nerve cell apoptosis

Objective:To study the changes of hs-CRP, S100B and NSE levels in serum and cerebrospinal fluid of children with epilepsy and their correlation with the nerve cell apoptosis.Methods:The children who were diagnosed with epilepsy in this hospital between March 2015 and February 2017 were selected as epilepsy group, and the children who underwent operation due to hernia during the same period were selected as control group. The cerebrospinal fluid was collected to determine the contents of hs-CRP, S100B and NSE, and the serum was collected to detect the contents of hs-CRP, S100B, NSE, apoptosis molecules and Sirtuins family molecules.Results: hs-CRP, S100B and NSE levels in serum and cerebrospinal fluid of epilepsy group were significantly higher than those of control group, Bim, Bax, Caspase-3, Caspase-4 and Caspase-9 levels in cerebrospinal fluid were significantly higher than those of control group, and XIAP, Bcl-2, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6 and SIRT7 levels in cerebrospinal fluid were significantly lower than those of control group;hs-CRP, S100B and NSE levels in serum and cerebrospinal fluid of children with epilepsy were positively correlated with Bim, Bax, Caspase-3, Caspase-4 and Caspase-9 levels in cerebrospinal fluid, and negatively correlated with XIAP, Bcl-2, SIRT1, SIRT2, SIRT3, SIRT4, SIRT5, SIRT6 and SIRT7 levels in cerebrospinal fluid.Conclusion: The abnormally elevated hs-CRP, S100B and NSE in serum and cerebrospinal fluid of children with epilepsy are closely related to the excessive apoptosis of nerve cells.

Development of component stiffness equations for thread-fixed one-side bolt connections to an enclosed rectangular hollow section column under tension

The derivation and validation of analytical equations for predicting the tensile initial stiffness of threadfixed one-side bolts(TOBs),connected to enclosed rectangular hollow section(RHS)columns,is presented in this paper.Two unknown stiffness components are considered:the TOBs connection and the enclosed RHS face.First,the trapezoidal thread of TOB,as an equivalent cantilevered beam subjected to uniformly distributed loads,is analyzed to determine the associated deformations.Based on the findings,the thread-shank serial-parallel stiffness model of TOB connection is proposed.For analysis of the tensile stiffness of the enclosed RHS face due to two bolt forces,the four sidewalls are treated as rotation constraints,thus reducing the problem to a two-dimensional plate analysis.According to the load superposition method,the deflection of the face plate is resolved into three components under various boundary and load conditions.Referring to the plate deflection theory of Timoshenko,the analytical solutions for the three deflections are derived in terms of the variables of bolt spacing,RHS thickness,height to width ratio,etc.Finally,the validity of the above stiffness equations is verified by a series of finite element(FE)models of T-stub substructures.The proposed component stiffness equations are an effective supplement to the component-based method.