Transcriptome changes in seeds during coleorhiza hair formation in rice

Coleorhiza hairs are hairlike structures in seeds of the grass family(Poaceae).The molecular mechanisms underlying its formation are largely unknown,study on this topic will expand our understanding of the effects of water status on germination during rice(Oryza sativa L.)direct seeding.Seeds of Nipponbare were treated under two water conditions:in one,half of the seed surface was immersed in water and the other half was embryo side in air(EIA),and in the other,the whole seed was covered by water(CBW).Coleorhiza hairs formed only in EIA samples.Transcriptomics was used to identify the gene regulation during coleorhiza hair formation in EIA(vs.CBW)embryos and endosperm.Embryos displayed more transcriptome modulation even though smaller in size than the endosperm.Differentially expressed genes(DEGs)were enriched in both primary and secondary metabolism and showed changes in abscisic acid,auxin,jasmonic acid,and salicylic acid signatures.Metabolites enrichment data were positively correlated with gene expression changes in the affected metabolic functional pathways.The presence of shorter coleorhiza hairs in an Os RHL1(Os06 g0184000,a coleorhiza hair formation regulation candidate gene)knockout mutant suggested that root hair-associated DEGs share molecular regulators that control the formation of coleorhiza hairs.

Emerging role of jasmonic acid in woody plant development

Jasmonic acid is a crucial phytohormone that plays a pivotal role,serving as a regulator to balancing plant development and resistance.However,there are analogous and distinctive characteristics exhibited in JA biosynthesis,perception,and signal transduction pathways in both herbaceous and woody plants.Moreover,the majority of research subjects have predominantly focused on the function of JA in model or herbaceous plants.Consequently,there is a significant paucity of studies investigating JA regulation networks in woody plants,particularly concerning post-transcriptional regulatory events such as alternative splicing(AS).This review article aims to conduct a comprehensive summary of advancements that JA signals regulate plant development across various woody species,comparing the analogous features and regulatory differences to herbaceous counterparts.In addition,we summarized the involvement of AS events including splicing factor(SF)and transcripts in the JA regulatory network,highlighting the effectiveness of high-throughput proteogenomic methods.A better understanding of the JA signaling pathway in woody plants has pivotal implications for forestry production,including optimizing plant management and enhancing secondary metabolite production.