期刊文献+
共找到3篇文章
< 1 >
每页显示 20 50 100
Small Interfering RNA Targeting MDR1 Inhibits Ovarian Cancer Growth and Increases Efficacy of Chemotherapy in vivo
1
作者 fu-jun liu Guo-lan Gao +2 位作者 Kai-jia Tu Li-qun Yu Jun Gao 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2009年第4期318-324,共7页
Objective: To further validate a knockdown approach for circumventing the multidrug resistance gene (MDR1), we used small interfering RNA(siRNA) targeting MDR1 gene to inhibit the expression of MDR1 gene and P-gl... Objective: To further validate a knockdown approach for circumventing the multidrug resistance gene (MDR1), we used small interfering RNA(siRNA) targeting MDR1 gene to inhibit the expression of MDR1 gene and P-glycoprotein(P-gp) in vivo. Methods: Ascite tumor xenografts were established by implanting human ovarian carcinoma cells SKOV3/AR intraperitoneally into the nude mice. The mice were randomized into the following three treatment groups with each group six mice respectively: Taxol, Taxol with lipofectamine and Taxol with siRNA/MDR1- lipofectamine intraperitoneal injection. The tumor growth rate and the ascite growth rate of mice were investigated. The expressions of MDR1 gene and P-gp in mice were determined by reverse transcription-polymerase chain reaction(RT-PCR) and immunohistochemistry respctively. Results: The growth of tumors and ascites in mice treated with Taxol and siRNA/MDR1- lipofectamine was significantly inhibited compared with those in mice of other groups. After 28 days' treatment, the average tumor weight and ascite volume decreased by 43.6% and 29.7% in the group treated with Taxol and siRNA/MDRl-lipofectamine compared with these treated with Taxol alone (P〈0.001). The expressions of MDR1 gene and P-gp in the group treated with Taxol and siRNA/MDRl-lipofectamine were also decreased compared with those in the group treated with Taxol alone (P〈0.001). Conclusion: Small interfering RNA targeting-MDR1 can effectively and specifically suppress the expression of MDRl(P-glycoprotein) and inhibit ovarian cancer growth in vivo. 展开更多
关键词 RNA interference siRNA MDR1 gene Ovarian cancer Nude mice
下载PDF
Characteristics, Management, and Outcomes of Acute Heart Failure in the Emergency Department: A Multicenter Registry Study with 1-year Follow-up in a Chinese Cohort in Beijing 被引量:23
2
作者 Guo-Gan Wang Si-Jia Wang +24 位作者 Jian Qin Chun-Sheng Li Xue-Zhong Yu Hong Shen Li-Pei Yang Yan Fu Ya-An Zheng Bin Zhao Dong-Min Yu fu-jun Qin De-Gui Zhou Ying Li fu-jun liu Wei Li Wei Zhao Xin Gao Zheng Wang Ming Jin Hong Zeng Yi Li Guo-Xing Wang Hong Zhou Xiao-Lu Sun Peng-Bo Wang Kam-Sang Woo 《Chinese Medical Journal》 SCIE CAS CSCD 2017年第16期1894-1901,共8页
Background: The emergency department (ED) has a pivotal influence on the management of acute heart failure (AHF), but dataconcerning current ED management are scarce. This Beijing AHF Registry Study investigated ... Background: The emergency department (ED) has a pivotal influence on the management of acute heart failure (AHF), but dataconcerning current ED management are scarce. This Beijing AHF Registry Study investigated the characteristics. ED management, and short- and long-term clinical outcomes of AHF. Methods: This prospective, multicenter, observational study consecutively enrolled 3335 AHF patients who visited 14 EDs in Beijing from January 1, 2011, to September 23, 2012. Baseline data on characteristics and management were collected in the EDs. Follow-up data on death and readmissions were collected until November 31, 2013, with a response rate of 92.80%. The data were reported as median (interquartile range) for the continuous variables, or as number (percentage) for the categorical variables. Results: The median age of the enrolled patients was 71 (58 79) years, and 46.84% wvere women. In patients with AHH coronary heart disease (43.27%) was the most common etiology, andmyocardium ischemia (30.22%) was the main precipitant. Most of the patients in the ED received intravenous treatments, including diuretics (79.28%) and vasodilators (74.90%). Fewer patients in the ED received neurohormonal antagonists, and 25.94%, 31.12%, and 33.73% of patients received angiotensin converting enzyme inhibitors/angiotensin receptor blockers, beta-blockers, and spironolactone, respectively. The proportions of patients who were admitted, discharged, left against medical advice, and died were 55.53%, 33.58%, 7.08%, and 3.81%, respectively. All-cause mortalities at 30 days and 1 year were 15.30% and 32.27%, respectively. Conclusions: Substantial details on characteristics and ED management of AHF were investigated. The clinical outcomes of AHF patients were dismal. Thus, further investigations of ED-based therapeutic approaches for AHF are needed. 展开更多
关键词 Acute Heart Failure Clinical Characteristics Clinical Outcomes Current Management Emergency Department
原文传递
Comparison of gene expression of the oncogenic Wnt/β-catenin signaling pathway components in the mouse and human epididymis
3
作者 Kai Wang Ning Li +7 位作者 Ching-Hei Yeung Trevor G Cooper Xue-Xia liu Juan liu Wen-Ting Wang Yan Li Hui Shi fu-jun liu 《Asian Journal of Andrology》 SCIE CAS CSCD 2015年第6期1006-1011,I0011,共7页
β-catenin is an integral part of the Wnt signaling pathway and has been linked to tumorigenesis and multiple developmental processes. The high β-catenin expression with low tumor incidence in the human epididymis is... β-catenin is an integral part of the Wnt signaling pathway and has been linked to tumorigenesis and multiple developmental processes. The high β-catenin expression with low tumor incidence in the human epididymis is thus intriguing. In the present study, the β-catenin gene and protein was found to be highly expressed in the murine caput epididymidis, and the protein mainly localized along the lateral plasma membranes of adjacent epithelial cells throughout both human and mouse epididymides. Furthermore, the adult mouse epididymis was found to express almost all the Wnt/β-catenin signaling pathway genes that were determined previously by our group in the human organ. Despite the differences in epididymal structure, the similar location of β-catenin and the high concordance of this pathway's components' gene expression in both the adult human and mouse epididymides make the mouse a suitable animal model for studying the anti-tumor mechanism of the epididymis. In addition, both the mRNA and protein expression of β-catenin shared a similar spatial expression as the mRNA of Rosl, a proto-oncogene and a key developmental regulator of the initial segment of the mouse epididymis. The observations on the parallel temporal expression of β-catenin and Rosl during postnatal development raise the possibility that the canonical Writ signaling pathway has an additional role in the postnatal development of mouse epididymis. 展开更多
关键词 EPIDIDYMIS postnatal development TUMORIGENESIS Wnt/β-catenin pathway Β-CATENIN
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部