[目的]观察柴黄清胰活血颗粒对重症急性胰腺炎(SAP)患者血清IL-1、IL-6、IL-8、TNF-α、IL-18水平的影响,探讨其治疗SAP的机制。[方法]选择2017年9月至2018年9月西南医科大学附属中医医院脾胃病科住院的SAP患者96例,随机分为治疗组51例...[目的]观察柴黄清胰活血颗粒对重症急性胰腺炎(SAP)患者血清IL-1、IL-6、IL-8、TNF-α、IL-18水平的影响,探讨其治疗SAP的机制。[方法]选择2017年9月至2018年9月西南医科大学附属中医医院脾胃病科住院的SAP患者96例,随机分为治疗组51例和对照组45例。对照组患者予西医综合治疗,治疗组在上述基础治疗上再联合柴黄清胰活血颗粒(由生大黄、黄芩、栀子、桃仁、赤芍、丹参、柴胡、白芍、枳实、厚朴、延胡索、黄芪、甘草、蒲公英组成)10 g+100 ml温开水口服及灌肠,每4 h 1次,比较两组患者治疗前后IL-1、IL-6、IL-8、TNF-α、IL-18水平及APACHEⅡ评分变化情况。[结果]治疗7天后,治疗组和对照组患者IL-1、IL-6、IL-8、TNF-α、IL-18水平均较治疗前明显下降(P<0.05),且治疗组较对照组下降更加明显(P<0.05)。治疗组和对照组患者APACHEⅡ评分均明显下降,且治疗组较对照组下降更加明显(P<0.05)。治疗组和对照组不良反应发生率比较差异无统计学意义(P>0.05)。[结论]在西医基础治疗上加用柴黄清胰活血颗粒可以有效降低患者APACHEⅡ评分和多种炎症因子水平,柴黄清胰活血颗粒治疗SAP作用机制可能与其降低血清IL-1、IL-6、IL-8、TNF-α、IL-18水平,阻止急性胰腺炎病情的进展有关。展开更多
OBJECTIVE：To investigate the changes in the neuronal microenvironment of the middle cerebral artery （MCA） territory induced by Jing-well points bloodletting acupuncture （WPBA） and to explore the neuroprotective m...OBJECTIVE：To investigate the changes in the neuronal microenvironment of the middle cerebral artery （MCA） territory induced by Jing-well points bloodletting acupuncture （WPBA） and to explore the neuroprotective mechanism of WPBA in stroke. METHODS： Adult male Sprague Dawley （n = 32） rats were randomly divided into four groups of eight animals each： WPBA-thalamus group （WT）, WPBA-caudate nucleus group （WC）, sham-control thalamus group （ST） and sham-control caudate nucleus group （SC）. Animals in the WT and WC groups received 2 I-μL of the extracellular tracer gadolinium-diethylene triamine pentaacetic acid （Gd-DTPA） injected into the thalamus or caudate nucleus, respectively, and 12 Jing-well points in the distal ends of the rats＇ digits were used for WPBA. Although 2 μL of Gd-DTPA was injected into the thalamus or caudate nucleus, respectively, for animals in the two sham groups （ST and SC）, no acupuncture or bloodletting was performed. Brain extracellular space and interstitial fluid flow parameters were measured using Gd-DTPA-enhanced magnetic reso- nance imaging. RESULTS： The brain interstitial fluid flow speed was decreased in the thalamus after WPBA, with a significantly lower Gd-DTPA clearance rate and longer half-life of Gd-DTPA in the thalamus of treated rats than those in sham-control rats [WPBA-treated rats＇clearance rate, （7.47 ± 3.15） x 10^-5/s （P = 0.009）; half-life, （1.52 ± 0.13） h, P = 0.000]. By contrast, no significant changes in brain extracellular space and interstitial fluid flow parameters were detected in the caudate nucleus after WPBA （P = 0.649）. In addi- tion, no differences in the morphology of the brain extracellular space or the final distribution of the traced brain interstitial fluid were demonstrated be- tween theWT and WC groups （P = 0.631, P = 0.970, respectively）. CONCLUSION： The WPBA decreased the speed of the local thalamic ISF flow in rats, which is assumed to be a beneficial protection by down-modulated the metabolic rate of the attacked neurons under stroke.展开更多
文摘[目的]观察柴黄清胰活血颗粒对重症急性胰腺炎(SAP)患者血清IL-1、IL-6、IL-8、TNF-α、IL-18水平的影响,探讨其治疗SAP的机制。[方法]选择2017年9月至2018年9月西南医科大学附属中医医院脾胃病科住院的SAP患者96例,随机分为治疗组51例和对照组45例。对照组患者予西医综合治疗,治疗组在上述基础治疗上再联合柴黄清胰活血颗粒(由生大黄、黄芩、栀子、桃仁、赤芍、丹参、柴胡、白芍、枳实、厚朴、延胡索、黄芪、甘草、蒲公英组成)10 g+100 ml温开水口服及灌肠,每4 h 1次,比较两组患者治疗前后IL-1、IL-6、IL-8、TNF-α、IL-18水平及APACHEⅡ评分变化情况。[结果]治疗7天后,治疗组和对照组患者IL-1、IL-6、IL-8、TNF-α、IL-18水平均较治疗前明显下降(P<0.05),且治疗组较对照组下降更加明显(P<0.05)。治疗组和对照组患者APACHEⅡ评分均明显下降,且治疗组较对照组下降更加明显(P<0.05)。治疗组和对照组不良反应发生率比较差异无统计学意义(P>0.05)。[结论]在西医基础治疗上加用柴黄清胰活血颗粒可以有效降低患者APACHEⅡ评分和多种炎症因子水平,柴黄清胰活血颗粒治疗SAP作用机制可能与其降低血清IL-1、IL-6、IL-8、TNF-α、IL-18水平,阻止急性胰腺炎病情的进展有关。
基金Supported by the National Natural Science Foundation of China(Related Mechanisms and Basic Research of Citicolinein Treatment of Ultra-Early Cerebral Ischemia Delivery via Brain Interstitial,No.81171080)the National Key Technology R&D Program for the 12th Five-year Plan(Advanced Cancer Treatment Equipment and Materials Development,No.2012BAI15B009)+1 种基金National Key Developmental Program for Scientific Instruments and Equipment(Study of Delivery via Brain Interstitial Based on Multimodal Molecular Imaging Technology,No.2011YQ030114)a Seeding Grant for Medicine and Information Sciences of Peking University(Disciplinarian and Mechanism Research of Information Transmission and Interaction between Somatosensory Stimulation and Brain Tissue Channel,No.2014-MI-12)
文摘OBJECTIVE：To investigate the changes in the neuronal microenvironment of the middle cerebral artery （MCA） territory induced by Jing-well points bloodletting acupuncture （WPBA） and to explore the neuroprotective mechanism of WPBA in stroke. METHODS： Adult male Sprague Dawley （n = 32） rats were randomly divided into four groups of eight animals each： WPBA-thalamus group （WT）, WPBA-caudate nucleus group （WC）, sham-control thalamus group （ST） and sham-control caudate nucleus group （SC）. Animals in the WT and WC groups received 2 I-μL of the extracellular tracer gadolinium-diethylene triamine pentaacetic acid （Gd-DTPA） injected into the thalamus or caudate nucleus, respectively, and 12 Jing-well points in the distal ends of the rats＇ digits were used for WPBA. Although 2 μL of Gd-DTPA was injected into the thalamus or caudate nucleus, respectively, for animals in the two sham groups （ST and SC）, no acupuncture or bloodletting was performed. Brain extracellular space and interstitial fluid flow parameters were measured using Gd-DTPA-enhanced magnetic reso- nance imaging. RESULTS： The brain interstitial fluid flow speed was decreased in the thalamus after WPBA, with a significantly lower Gd-DTPA clearance rate and longer half-life of Gd-DTPA in the thalamus of treated rats than those in sham-control rats [WPBA-treated rats＇clearance rate, （7.47 ± 3.15） x 10^-5/s （P = 0.009）; half-life, （1.52 ± 0.13） h, P = 0.000]. By contrast, no significant changes in brain extracellular space and interstitial fluid flow parameters were detected in the caudate nucleus after WPBA （P = 0.649）. In addi- tion, no differences in the morphology of the brain extracellular space or the final distribution of the traced brain interstitial fluid were demonstrated be- tween theWT and WC groups （P = 0.631, P = 0.970, respectively）. CONCLUSION： The WPBA decreased the speed of the local thalamic ISF flow in rats, which is assumed to be a beneficial protection by down-modulated the metabolic rate of the attacked neurons under stroke.
