The authors regret to inform that four images/graphs in Fig.3 and 6 lacked order annotations of A/B/C/D.And the order annotations of cell viability of HUVECs and HUASMCs are opposite in Fig.6.The correct form of the t...The authors regret to inform that four images/graphs in Fig.3 and 6 lacked order annotations of A/B/C/D.And the order annotations of cell viability of HUVECs and HUASMCs are opposite in Fig.6.The correct form of the two figures are as below.The authors apologize for any inconvenience caused and state that this does not change the discussion and the scientific conclusions of the article.展开更多
Nitric oxide(NO)and hydrogen sulfide(H_(2)S)gasotransmitters exhibit potential therapeutic effects in the car-diovascular system.Herein,biomimicking multilayer structures of biological blood vessels,bilayer smalldiame...Nitric oxide(NO)and hydrogen sulfide(H_(2)S)gasotransmitters exhibit potential therapeutic effects in the car-diovascular system.Herein,biomimicking multilayer structures of biological blood vessels,bilayer smalldiameter vascular grafts(SDVGs)with on-demand NO and H_(2)S release capabilities,were designed and fabri-cated.The keratin-based H_(2)S donor(KTC)with good biocompatibility and high stability was first synthesized and then electrospun with poly(L-lactide-co-caprolactone)(PLCL)to be used as the outer layer of grafts.The elec-trospun poly(ε-caprolactone)(PCL)mats were aminolyzed and further chelated with copper(II)ions to construct glutathione peroxidase(GPx)-like structural surfaces for the catalytic generation of NO,which acted as the inner layer of grafts.The on-demand release of NO and H_(2)S selectively and synergistically promoted the proliferation and migration of human umbilical vein endothelial cells(HUVECs)while inhibiting the proliferation and migration of human umbilical artery smooth muscle cells(HUASMCs).Dual releases of NO and H_(2)S gaso-transmitters could enhance their respective production,resulting in enhanced promotion of HUVECs and inhi-bition of HUASMCs owing to their combined actions.In addition,the bilayer grafts were conducive to forming endothelial cell layers under flow shear stress.In rat abdominal aorta replacement models,the grafts remained patency for 6 months.These grafts were capable of facilitating rapid endothelialization and alleviating neo-intimal hyperplasia without obvious injury,inflammation,or thrombosis.More importantly,the grafts were expected to avoid calcification with the degradation of the grafts.Taken together,these bilayer grafts will be greatly promising candidates for SDVGs with rapid endothelialization and anti-calcification properties.展开更多
The authors regret to inform that four images/graphs in Fig.3 and 6 lacked order annotations of A/B/C/D.And the order annotations of cell viability of HUVECs and HUASMCs are opposite in Fig.6.The correct form of the t...The authors regret to inform that four images/graphs in Fig.3 and 6 lacked order annotations of A/B/C/D.And the order annotations of cell viability of HUVECs and HUASMCs are opposite in Fig.6.The correct form of the two figures are as below.The authors apologize for any inconvenience caused and state that this does not change the discussion and the scientific conclusions of the article.展开更多
文摘The authors regret to inform that four images/graphs in Fig.3 and 6 lacked order annotations of A/B/C/D.And the order annotations of cell viability of HUVECs and HUASMCs are opposite in Fig.6.The correct form of the two figures are as below.The authors apologize for any inconvenience caused and state that this does not change the discussion and the scientific conclusions of the article.
基金supported by the National Natural Science Fund of China(81873923)Jiangsu Higher Education Institutions(19KJA310001 and PAPD)Jiangsu Collaborative Innovation Center of Biomedical Functional Materials.
文摘Nitric oxide(NO)and hydrogen sulfide(H_(2)S)gasotransmitters exhibit potential therapeutic effects in the car-diovascular system.Herein,biomimicking multilayer structures of biological blood vessels,bilayer smalldiameter vascular grafts(SDVGs)with on-demand NO and H_(2)S release capabilities,were designed and fabri-cated.The keratin-based H_(2)S donor(KTC)with good biocompatibility and high stability was first synthesized and then electrospun with poly(L-lactide-co-caprolactone)(PLCL)to be used as the outer layer of grafts.The elec-trospun poly(ε-caprolactone)(PCL)mats were aminolyzed and further chelated with copper(II)ions to construct glutathione peroxidase(GPx)-like structural surfaces for the catalytic generation of NO,which acted as the inner layer of grafts.The on-demand release of NO and H_(2)S selectively and synergistically promoted the proliferation and migration of human umbilical vein endothelial cells(HUVECs)while inhibiting the proliferation and migration of human umbilical artery smooth muscle cells(HUASMCs).Dual releases of NO and H_(2)S gaso-transmitters could enhance their respective production,resulting in enhanced promotion of HUVECs and inhi-bition of HUASMCs owing to their combined actions.In addition,the bilayer grafts were conducive to forming endothelial cell layers under flow shear stress.In rat abdominal aorta replacement models,the grafts remained patency for 6 months.These grafts were capable of facilitating rapid endothelialization and alleviating neo-intimal hyperplasia without obvious injury,inflammation,or thrombosis.More importantly,the grafts were expected to avoid calcification with the degradation of the grafts.Taken together,these bilayer grafts will be greatly promising candidates for SDVGs with rapid endothelialization and anti-calcification properties.
文摘The authors regret to inform that four images/graphs in Fig.3 and 6 lacked order annotations of A/B/C/D.And the order annotations of cell viability of HUVECs and HUASMCs are opposite in Fig.6.The correct form of the two figures are as below.The authors apologize for any inconvenience caused and state that this does not change the discussion and the scientific conclusions of the article.
