Dear Editor,To establish a more specific diagnostic assay than thecurrent methods for rabies virus(RABV)infection in ca-nines and wildlife species,we developed a SYBR-GreenI quantitative real-time reverse transcriptio...Dear Editor,To establish a more specific diagnostic assay than thecurrent methods for rabies virus(RABV)infection in ca-nines and wildlife species,we developed a SYBR-GreenI quantitative real-time reverse transcription-PCR(RT-PCR).Three primers were designed and synthesized:oneof which,located within the leader sequences and target-ed for the whole viral genome amplification to obtain展开更多
基金supported by National High Technology and Development Project("863")(Approval No.2012AA101303)Special Fund for Agro-scientific Research in the Public Interest(Approval No.201203056)
文摘Dear Editor,To establish a more specific diagnostic assay than thecurrent methods for rabies virus(RABV)infection in ca-nines and wildlife species,we developed a SYBR-GreenI quantitative real-time reverse transcription-PCR(RT-PCR).Three primers were designed and synthesized:oneof which,located within the leader sequences and target-ed for the whole viral genome amplification to obtain
