眼睑钙化上皮瘤1例

患者,女,52岁。因发现右眼上睑皮肤红肿20 d,于2022年5月就诊于山东第一医科大学附属眼科医院。患者20 d前无明显诱因出现右眼上睑皮肤红肿,就诊于外院,诊断为睑板腺囊肿,给予妥布霉素地塞米松眼膏治疗,未见明显好转。患者有甲减病史10余年,否认重大全身性疾病,否认药物过敏史,否认家族性遗传病史。眼部检查:右眼最佳矫正视力1.0,眼压11 mmHg(1 mmHg=0.133 kPa),右眼上睑近中央睑缘处见一直径约5 mm半球形隆起(图1A),质韧,局部皮肤稍有淤青、淤血,压痛阴性,结膜无明显充血,角膜透明,前房中等深浅,瞳孔圆,晶状体透明;左眼检查未见明显阳性体征。

Effects of gibberellin priming on seedling emergence and transcripts involved in mesocotyl elongation in rice under deep direct-seeding conditions

Mesocotyl elongation is a key trait influencing seedling emergence and establishment in direct-seeding rice cultivation.The phytohormone gibberellin(GA)has positive effects on mesocotyl elongation in rice.However,the physiological and molecular basis underlying the regulation of mesocotyl elongation mediated by GA priming under deep-sowing conditions remains largely unclear.In the present study,we performed a physiological and comprehensive transcriptomic analysis of the function of GA priming in mesocotyl elongation and seedling emergence using a direct-seeding japonica rice cultivar ZH10 at a5-cm sowing depth.Physiological experiments indicated that GA priming significantly improved rice seedling emergence by increasing the activity of starch-metabolizing enzymes and compatible solute content to supply the energy essential for subsequent development.Transcriptomic analysis revealed 7074 differentially expressed genes(false discovery rate of<0.05,|log2(fold change)|of≥1)after GA priming.Furthermore,gene ontology(GO)and Kyoto encyclopedia of genes and genomes(KEGG)enrichment analyses revealed that genes associated with transcriptional regulation,plant hormone biosynthesis or signaling,and starch and sucrose metabolism were critical for GA-mediated promotion of rice mesocotyl elongation.Further analyses showed that the expression of the transcription factor(TF)genes(v-myb avian myeloblastosis viral oncogene homolog(MYB)alternative splicing 1(MYBAS1),phytochrome-interacting factors 1(PIF1),Oryza sativa teosinte branched 1/cycloidea/proliferating cell factor 5(Os TCP5),slender 1(SLN1),and mini zinc finger 1(MIF1)),plant hormone biosynthesis or signaling genes(brassinazole-resistant 1(BZR1),ent-kaurenoic acid oxidase-like(KAO),GRETCHEN HAGEN 3.2(GH3.2),and small auxin up RNA 36(SAUR36)),and starch and sucrose metabolism genes(α-amylases(AMY2 A and AMY1.4))was highly correlated with the mesocotyl elongation and deep-sowing tolerance response.These results enhance our understanding of how nutrient metabolism-related substances and genes regulate rice mesocotyl elongation.This may facilitate future studies on related genes and the development of novel rice varieties tolerant to deep sowing.

Time effects of rice straw and engineered bacteria on reduction of exogenous Cu mobility in three typical Chinese soils

Globally,copper(Cu)accumulation in soils is a major environmental concern.Agricultural organic waste and some bacterial species can readily absorb metals in an eco-friendly manner,and thus are commonly used in metal-contaminated soil remediation.This study investigates the change in Cu fractions during the aging process and the time effects of rice straw(RS)and engineered bacteria(EB)(Pseudomonas putida X4/pIME)on reduction of Cu mobility.Three typical Chinese soils(red,cinnamon,and black soils)were incubated with RS or RS+EB in the presence of exogenous Cu for 24 months.The soil physicochemical properties,reactive soil components,Cu fractions,and Cu mobility were determined over time.The Cu mobility factor(MF)values were the lowest in the black soil(6.4-9.2)because of its high organic carbon and clay contents.The additions of both RS and RS+EB accelerated Cu stabilization during the aging process in all three soils.The Cu MF values decreased with time during the initial 20 months;however,the MF values increased thereafter in all soils,which might be due to the reduction of humic substances and amorphous iron oxides and the increase in iron oxides complexed on the organic matter.The reduction rates of Cu MF were similar after 16,24,and more than 24 months in the red,cinnamon,and black soils,respectively,indicating that RS and RS+EB could limit Cu mobility at different times in various soils.The RS treatment showed the greatest efficiency in reducing Cu mobility in the red,cinnamon,and black soils after 12,12,and 8 months of incubation,respectively.The RS+EB treatment was more efficient than the RS treatment in the red soil during the initial 8 months of the incubation period.Our study provides theoretical support for Cu risk assessments and RS supplementation for Cu remediation in different soils.

巨大副泪腺潴留囊肿1例

患者,男,69岁,因"右眼上睑肿物生长8个月余"于2020年8月26日就诊于山东第一医科大学附属眼科医院。患者身体状况良好,否认家族中传染性疾病及遗传性疾病。既往6年前因"声带肿物"行手术治疗,2年半前因"右下肢静脉血栓"行手术治疗。患者8个多月前出现右上侧"牙齿碎片"脱落,当时无疼痛等不适,时隔20 d先后出现右侧鼻唇沟疼痛和右眼上睑红肿。

Current situation,efficacy,and safety of extracorporeal cardiopulmonary resuscitation in China

To the Editor:The survival rate from conventional cardiopulmonary resuscitation(CCPR)of out-of-hospital and in-hospital cardiac arrest ranges from 9%to 20%.[1]Without returning of spontaneous circulation(ROSC)after CCPR,the chance of recovery is very poor.Initiating venoarterial extracorporeal membrane oxygenation in those patients may provide adequate organ perfusion that allows for longer organ viability and permit time for therapies seeking to correct the inciting pathologic event,which is termed extracorporeal cardiopulmonary resuscitation(ECPR).[2]Time to treatment,ECPR-related complications,as well as etiology,have been recognized as the main predictors of survival for ECPR patients.[3]ECPR has been carried out more and more in China,but the information on ECPR application remains unknown.Therefore,we conducted a retrospective survey about the usage of ECPR in China,including patient selection,initiation and management of extracorporeal mechanical ventilation(ECMO),patient outcome,and compared the status during 2017 and 2020.