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小鼠生长激素受体基因环状转录本的克隆及其表达规律 被引量:1
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作者 张为露 冷奇颖 +4 位作者 郑嘉辉 Ali Hassan Nawaz 焦振海 王府建 张丽 《遗传》 CAS CSCD 北大核心 2021年第9期890-900,共11页
根据文献报道并通过circBase数据库查找,发现小鼠生长激素受体基因(growth hormone receptor,GHR)存在8个环状转录本。为确定GHR基因环状转录本(circGHR)的真实存在,探究其表达规律,本研究以昆明小鼠(Mus musculus)为研究对象,通过PCR... 根据文献报道并通过circBase数据库查找,发现小鼠生长激素受体基因(growth hormone receptor,GHR)存在8个环状转录本。为确定GHR基因环状转录本(circGHR)的真实存在,探究其表达规律,本研究以昆明小鼠(Mus musculus)为研究对象,通过PCR扩增和测序检测circGHR的真实存在,并筛选出一个circGHR作为目标分子,通过RNase R处理和反转录证实了circGHR的环形结构,利用qRT-PCR分析circGHR和GHR mRNA的时空表达规律。结果表明:小鼠circGHR全长为820 nt,由GHR基因外显子2~8环化形成。RNase R耐受性分析表明,小鼠circGHR具备环形分子的一般特征,不易被RNase R降解。与oligo-d(T)18引物相比,随机引物对circGHR具有较高的反转录效率,进一步说明circGHR是一个不含poly(A)的环状结构分子。组织表达谱结果表明,circGHR在1周龄和7周龄小鼠肝脏和肾脏高表达,在胸肌和腿肌中低表达;circGHR在肝脏和胸肌组织的时序表达谱结果表明circGHR表达无显著差异;circGHR在腿肌组织的时序表达谱结果表明,circGHR在小鼠5周龄之前为低表达,在7周龄以后表现为高表达。本研究结果证实了小鼠GHR基因存在一个环状转录本circGHR,并初步揭示了circGHR的表达规律,为后期深入开展小鼠circGHR的生物学功能及其在小鼠生长发育过程中的作用机制奠定基础。 展开更多
关键词 小鼠 生长激素受体 环状RNA
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Uncovering the CO_(2)emissions of vehicles:A well-to-wheel approach
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作者 Zuoming Zhang Hongyang Su +3 位作者 Wenbin Yao fujian wang Simon Hu Sheng Jin 《Fundamental Research》 CAS CSCD 2024年第5期1025-1035,共11页
Carbon dioxide(CO_(2))from road traffic is a non-negligible part of global greenhouse gas(GHG)emissions,and it is a challenge for the world today to accurately estimate road traffic CO_(2)emissions and formulate effec... Carbon dioxide(CO_(2))from road traffic is a non-negligible part of global greenhouse gas(GHG)emissions,and it is a challenge for the world today to accurately estimate road traffic CO_(2)emissions and formulate effective emission reduction policies.Current emission inventories for vehicles have either low-resolution,or limited coverage,and they have not adequately focused on the CO_(2)emission produced by new energy vehicles(NEV)considering fuel life cycle.To fill the research gap,this paper proposed a framework of a high-resolution well-to-wheel(WTW)CO_(2)emission estimation for a full sample of vehicles and revealed the unique CO_(2)emission characteristics of different categories of vehicles combined with vehicle behavior.Based on this,the spatiotemporal characteristics and influencing factors of CO_(2)emissions were analyzed with the geographical and temporal weighted regression(GTWR)model.Finally,the CO_(2)emissions of vehicles under different scenarios are simulated to support the formulation of emission reduction policies.The results show that the distribution of vehicle CO_(2)emissions shows obvious heterogeneity in time,space,and vehicle category.By simply adjusting the existing NEV promotion policy,the emission reduction effect can be improved by 6.5%-13.5%under the same NEV penetration.If combined with changes in power generation structure,it can further release the emission reduction potential of NEVs,which can reduce the current CO_(2)emissions by 78.1%in the optimal scenario. 展开更多
关键词 Carbon neutrality Well-to-wheel emission Emission characteristics License plate recognition data Geographical and temporal weighted regression model Emission reduction policy
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