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南京地区某渔场嗜水气单胞菌流行菌株的鉴定及分子分型 被引量:10
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作者 曹青 张向阳 +4 位作者 庞茂达 王楠楠 furqan awan 陆承平 刘永杰 《水产学报》 CAS CSCD 北大核心 2017年第1期134-141,共8页
为确定南京地区某渔场异育银鲫出血性败血症的病原体,采集该渔场3个饲养塘口患病鱼脏器进行细菌分离培养,基于气单胞菌DNA促旋酶B亚单位(gyr B)基因序列设计引物,PCR扩增后测序进行细菌鉴定;同时,对病原菌生物学特性如溶血性、蛋白酶活... 为确定南京地区某渔场异育银鲫出血性败血症的病原体,采集该渔场3个饲养塘口患病鱼脏器进行细菌分离培养,基于气单胞菌DNA促旋酶B亚单位(gyr B)基因序列设计引物,PCR扩增后测序进行细菌鉴定;同时,对病原菌生物学特性如溶血性、蛋白酶活性、生物被膜形成能力、运动能力以及对斑马鱼的毒力等进行分析,并进行多位点序列分型(MLST)。结果在3个饲养塘口均分离到嗜水气单胞菌,分别命名为GL-15、GL-17和GL-18;MLST分型显示三株分离菌均为ST251型;生物学特性实验表明,均有溶血性、蛋白酶活性以及运动性,生物被膜形成能力较强,OD595均超过0.5,对斑马鱼的LD50在1.4×103~3.9×103 CFU/尾,属强毒株。本研究为有效防控南京地区嗜水气单胞菌引发的出血性败血症的暴发流行提供了依据。 展开更多
关键词 嗜水气单胞菌 鉴定 ST251 生物学特性
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Some challenges in progressive control of livestock originated zoonotic diseases in Pakistan-a pilot survey
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作者 furqan awan Muhammad Muddassir Ali +2 位作者 Umair Iqbal Irfan Khattak Muhammad Hassan Mushtaq 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2014年第10期821-824,共4页
Objective:To document the recent livestock related practices and possible unhygienic ways of pathogen entry.Identification of the potential risk factors for the spread of infection is important to design an evidence-b... Objective:To document the recent livestock related practices and possible unhygienic ways of pathogen entry.Identification of the potential risk factors for the spread of infection is important to design an evidence-based disease control programme.Methods:Rapid assessment method was adopted and a purposive sample of 60 dairy farmers were interviewed.The following factors were noted for contributing in primary and secondary transmission of zoonotic infections:(i) persons who come in close contact with animals and their secretions,(ii) management strategies of farm animals(sheds and environment),(iii) management practices adopted at farms,(iv) small scale farmers and rural livestock production systems,(v)milk collection systems.Results:This research unveiled the certain routes of zoonotic disease transmission.Certain management practices,precautionary measures and strategies were the pivotal risk factors.Conclusions:The study emphasizes the need to educate the poor livestock keepers. 展开更多
关键词 ZOONOSIS Risk assessment LIVESTOCK Emerging INFECTIONS
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