The ability to resolve the spatio-temporal complexity of intracelular O_(2) distribution is the“HolyGrail” of cellular physiology.In an effort to obtain a minimally invasive approach to the mappingof intracllar O,te...The ability to resolve the spatio-temporal complexity of intracelular O_(2) distribution is the“HolyGrail” of cellular physiology.In an effort to obtain a minimally invasive approach to the mappingof intracllar O,tensions,two methods of phosphorescent lifetime imaging microscopy werecompared in the current study and gave similar results.These were two-photon confocal laserscanning microscopy with pinhole shifting,and picosecond time-resolved epi-phosphorescencemicroscopy using a single 0.5μm focused spot.Both methods utilized Ru coordination complexembedded nanopartidles(45 nm diameter)as the phosphorescent probe,excited using pulsedoutputs of a titanium sapphire Tsunami lasers(710-1050 nm).展开更多
文摘The ability to resolve the spatio-temporal complexity of intracelular O_(2) distribution is the“HolyGrail” of cellular physiology.In an effort to obtain a minimally invasive approach to the mappingof intracllar O,tensions,two methods of phosphorescent lifetime imaging microscopy werecompared in the current study and gave similar results.These were two-photon confocal laserscanning microscopy with pinhole shifting,and picosecond time-resolved epi-phosphorescencemicroscopy using a single 0.5μm focused spot.Both methods utilized Ru coordination complexembedded nanopartidles(45 nm diameter)as the phosphorescent probe,excited using pulsedoutputs of a titanium sapphire Tsunami lasers(710-1050 nm).
