The author investigated the antioxidant effect of irbesartan[2-butyl-3-（{4-[2-（2H-l,2,3,4-tetrazol-5-yl）. phenyl]phenyl}methyl）-1,3-diazaspiro[4.4]non-1-en-4-one], an angiotensin receptor blocker（ARB）, on the ox...The author investigated the antioxidant effect of irbesartan[2-butyl-3-（{4-[2-（2H-l,2,3,4-tetrazol-5-yl）. phenyl]phenyl}methyl）-1,3-diazaspiro[4.4]non-1-en-4-one], an angiotensin receptor blocker（ARB）, on the oxidation of erythrocytes induced by 2,2-[azobis（2-amidinopropane） hydrochloride]（AAPH） and H2O2 The value of half concentration（IC50） of irbesartan to scavenge radicals was measured by reacting it with 2,2-[azinobis（3-ethylbenzothia- zoline-6-sulfonate） radical eation]（ABTS＋）. Activities of the catalase（CAT）, superoxide dismutase（SOD）, glutathione peroxidase（GSH-Px） and the content of malondialdehyde（MDA） in liver tissue and blood serum of normal-rats were measured by means of speetrophotometry to study the antioxidation function of irbesartan. Results of experiments show that irbesartan can scavenge ABTS＋ and superoxide radicals effectively as well as inhibit AAPH-, H2O2-induced hemolysis of erythrocytes. Irbesartan can also increase the activities of GSH-Px, SOD, CAT and decrease the content of MDA of normal rats. So irbesartan is a good antioxidant.展开更多
文摘The author investigated the antioxidant effect of irbesartan[2-butyl-3-（{4-[2-（2H-l,2,3,4-tetrazol-5-yl）. phenyl]phenyl}methyl）-1,3-diazaspiro[4.4]non-1-en-4-one], an angiotensin receptor blocker（ARB）, on the oxidation of erythrocytes induced by 2,2-[azobis（2-amidinopropane） hydrochloride]（AAPH） and H2O2 The value of half concentration（IC50） of irbesartan to scavenge radicals was measured by reacting it with 2,2-[azinobis（3-ethylbenzothia- zoline-6-sulfonate） radical eation]（ABTS＋）. Activities of the catalase（CAT）, superoxide dismutase（SOD）, glutathione peroxidase（GSH-Px） and the content of malondialdehyde（MDA） in liver tissue and blood serum of normal-rats were measured by means of speetrophotometry to study the antioxidation function of irbesartan. Results of experiments show that irbesartan can scavenge ABTS＋ and superoxide radicals effectively as well as inhibit AAPH-, H2O2-induced hemolysis of erythrocytes. Irbesartan can also increase the activities of GSH-Px, SOD, CAT and decrease the content of MDA of normal rats. So irbesartan is a good antioxidant.
