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椭圆度及壁厚不均度对套管抗挤强度的影响 被引量:1
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作者 高建昌 金轩 高连新 《钢管》 CAS 2023年第2期23-27,共5页
随着各类石油套管对可靠性和使用寿命要求的不断提高,对石油套管的抗挤能力分析提出了更高的要求。影响套管抗挤能力的参数除了材料本身的强度以外,还包括套管的椭圆度、壁厚不均度等。以φ101.6 mm×7.8 mm规格的试样套管为对象,... 随着各类石油套管对可靠性和使用寿命要求的不断提高,对石油套管的抗挤能力分析提出了更高的要求。影响套管抗挤能力的参数除了材料本身的强度以外,还包括套管的椭圆度、壁厚不均度等。以φ101.6 mm×7.8 mm规格的试样套管为对象,建立套管抗挤强度的计算模型,利用有限元方法对套管的抗挤强度进行模拟计算,分析与验证椭圆度和壁厚不均度这两个参数对套管抗挤强度的影响,从而为合理选用套管以及开发高抗挤套管提供经验与指导。 展开更多
关键词 石油套管 抗挤强度 椭圆度 壁厚不均度 有限元模拟
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陕西杨凌番茄褪绿病毒的分子检测 被引量:6
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作者 王志荣 李晓东 +5 位作者 朱玉 黄泽军 高建昌 国艳梅 杜永臣 王孝宣 《植物保护》 CAS CSCD 北大核心 2018年第2期81-88,共8页
近年来在陕西番茄生产区出现了一种新病害,病株表现为叶片褪绿,叶脉颜色变深及叶片增厚,果实变小发白,严重影响番茄产量及经济效益,2016年发病极为严重,部分产区甚至绝收。该病疑似由番茄褪绿病毒Tomato chlorosis virus(ToCV)引起。利... 近年来在陕西番茄生产区出现了一种新病害,病株表现为叶片褪绿,叶脉颜色变深及叶片增厚,果实变小发白,严重影响番茄产量及经济效益,2016年发病极为严重,部分产区甚至绝收。该病疑似由番茄褪绿病毒Tomato chlorosis virus(ToCV)引起。利用ToCV特异引物对感病番茄样品进行RT-PCR检测,结果从所采集的4份病样中均检测到ToCV预期大小的特异片段。对ToCV外壳蛋白CP基因和类热激蛋白HSP70h基因进行克隆与序列分析,ToCV-YL1 CP基因774个核苷酸序列与已报道的ToCVCP基因有较高的一致性,与山东青岛和山西晋中分离物同源性为100%。ToCV-YL1 HSP70h基因1 665个核苷酸序列与已报道的ToCV HSP70h基因有较高的同源性,与中国、韩国、日本、美国、希腊分离物同源性达到99%以上。研究表明ToCV已传播至陕西地区。 展开更多
关键词 番茄 番茄褪绿病毒病 CP基因 HSP70h基因
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西瓜子叶离体再生体系的建立
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作者 牛波 张晨光 +4 位作者 刘阳 张思远 谢露露 高建昌 李美阳 《中国蔬菜》 北大核心 2022年第3期78-82,共5页
为建立高效西瓜子叶离体再生培养体系,以小果型西瓜品种香秀为试验材料,选取无菌培养4~5 d的子叶为外植体,研究了外源激素6-BA、NAA、IAA和IBA对西瓜子叶离体再生的影响。结果表明,诱导不定芽的最佳培养基配比是MS+2.25 mg·L^(-1)6... 为建立高效西瓜子叶离体再生培养体系,以小果型西瓜品种香秀为试验材料,选取无菌培养4~5 d的子叶为外植体,研究了外源激素6-BA、NAA、IAA和IBA对西瓜子叶离体再生的影响。结果表明,诱导不定芽的最佳培养基配比是MS+2.25 mg·L^(-1)6-BA+0.1 mg·L^(-1) NAA,诱导率为90.56%;不定芽伸长的最佳培养基配比是MS+1.0 mg·L^(-1)6-BA+0.50 mg·L^(-1) IAA;生根的最佳培养基配比是1/2MS+1.0 mg·L^(-1) IBA,生根率可达到93.33%。经炼苗移栽后再生苗的存活率可达到98.33%。从子叶接种到获得可移栽的再生苗约需63 d。 展开更多
关键词 西瓜 子叶 离体再生
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Identification and Characterization of microRNA during Bemisia tabaci Infestations in Solanum lycopersicum and Solanum habrochaites 被引量:7
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作者 WANG Ketao SU Xiaomei +3 位作者 CUI Xia DU Yongchen ZHANG Shuaibin gao jianchang 《Horticultural Plant Journal》 SCIE 2018年第2期62-72,共11页
MicroRNAs(miRNAs) are a class of small non-coding RNAs that can regulate target gene expression during many plant growth and development processes. In recent years, several studies identified the miRNAs involved in fr... MicroRNAs(miRNAs) are a class of small non-coding RNAs that can regulate target gene expression during many plant growth and development processes. In recent years, several studies identified the miRNAs involved in fruit development, leaf development, and abiotic responses in tomato. However, little is known about the miRNAs that respond to insect attack. Here, miRNAs were identified by high-throughput sequencing at different stages after infections by the whitefly Bemisia tabaci in Solanum lycopersicum and Solanum habrochaites, which are susceptible and resistant to whitefly, respectively. A total of 44 known miRNA families were identified, and 33 were shared between the two species. Among these, 13 miRNA families were newly reported in tomato. After strict filtering, some novel miRNAs were also discovered. The global expression patterns of the miRNAs exhibited were different between the two species, reflecting their different responses and resistance levels to whitefly attack. Some of the predicted target genes of differentially expressed miRNAs may be involved in responding to, and defending against, diseases and insects. Thus, plant miRNAs are important in the responses to, and resistance against, insects and provide a useful resource for further investigations into the mechanism of miRNA-mediated plant–insect interactions. 展开更多
关键词 Solanum lycopersicum Solanum habrochaites Bemisia tabaci MIRNAS whitefly infection high-throughput sequencing
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Identificatio of Quantitative Trait Loci for Fruit Weight, Soluble Solids Content, and Plant Morphology Using an Introgression Line Population of Solanum pennellii in a Fresh Market Tomato Inbred Line
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作者 ZHOU Hui WANG Xiaoxuan +4 位作者 HUANG Zejun gao jianchang GUO Yanmei DU Yongchen HU Hong 《Horticultural Plant Journal》 SCIE 2016年第1期26-34,共9页
Introgression lines are convenient populations for the identification fine-mapping and functional analysis of genes that are responsible for variations in traits, particularly quantitative trait genes. An introgressio... Introgression lines are convenient populations for the identification fine-mapping and functional analysis of genes that are responsible for variations in traits, particularly quantitative trait genes. An introgression line population of Solanum pennellii LA0716 in a fresh market tomato inbred line 1052 was developed by our group. This population was composed of 214 lines. In the present study, fi e quantitative trait loci(QTLs)for fruit weight, two QTLs for soluble solids content(SSC), three QTLs for plant height, and one QTL for leaf size were identifie using this introgression line population. Among these, fw3 a and fw4 a for fruit weight, ssc7 a for SSC, h4t2 a, h4t3 a, and h4t7 a for plant height, and lz12 a for leaf size were determined to be novel loci. These results serve as a foundation for fine-mappin and functional analysis of genes underlying these QTLs. 展开更多
关键词 tomato INTROGRESSION line quantitative TRAIT LOCUS fruit weight soluble solids content plant morphology
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