基于望目辨证理论和白睛成像AI技术探讨高胆固醇血症的目络特征及发病机制相关性研究

目的基于望目辨证理论和白睛成像AI技术分析高胆固醇血症的目络特征及发病机制的关联性研究。方法纳入甘肃中医药大学附属医院门诊及住院部接受检查的受试者100例,其中高胆固醇血症(TC)组58例,胆固醇水平正常(NC)组42例。两组均接受研究人员使用“MyEyeD-10白睛无影成像健康智能分析系统”[又称目诊仪(械注准20182160164)]进行对白睛眼象特征的高清采集、特征提取和综合分析,进行数据统计TC组和NC组的中医证型、目络特征形态、目络血脉颜色、目络病变分布区域。结果TC组的中医证型是痰浊内阻(46.55%,27/58)显著高于NC组(23.81%,10/42)(P<0.05);TC组的主要目络形态特征“斑”特征分值(4.24±2.63)显著高于NC组(2.92±1.43)(P<0.05);TC组的“血脉”特征分值(12.88±7.33)显著高于NC组(6.54±3.92)(P<0.05);TC组目络血脉颜色“黯红色”,特征分值(11.21±5.46)显著高于NC组(7.29±7.13)(P<0.05);TC组目络区域特征为“A”区(黑睛下方,对应脏腑区域“脾与胃”),特征分值(12.14±6.87)显著高于NC组(6.86±4.93)(P<0.05)。结论高胆固醇血症引起目络疾病的机制是可能与机体伤于脾胃,脾失运化,久则痰湿浊瘀内生,浮现于目络而出现“斑”“血脉黯红”相关病变有关联,为中医望目辨证的客观化、精准化提供参考依据。

低磷胁迫对菘蓝幼苗叶片生理特性的影响

为研究低磷胁迫对菘蓝幼苗叶片的影响,本研究将菘蓝种子种于以蛭石为基质的小花盆中,用含不同浓度(0 mmol/L、0.05 mmol/L、0.5 mmol/L)KH_(2)PO_(4)的霍格兰营养液处理13 d,培养条件为温度25℃、光照强度80μmol/(m^(2)·s)、相对湿度为75%、光照时间为12 h光照/12 h黑暗,观察生理特性的变化。结果显示,KH_(2)PO_(4)浓度为0.5 mmol/L时,菘蓝幼苗生长状态最好,叶片长度可达4.5 cm;KH_(2)PO_(4)浓度低于0.5 mmol/L时,随着KH_(2)PO_(4)浓度的降低(0.05 mmol/L、0 mmol/L),菘蓝幼苗叶片的生理特性显著变化,与对照相比,呼吸速率、净光合速率、气孔导度、胞间二氧化碳浓度、总叶绿素含量和含水量分别下降了53.8%和30.8%、61.3%和38%、72.3%和33.1%、45.1%和27.2%、38.3%和13.3%、6.7%和3.7%;总可溶性糖含量、脯氨酸含量、电导率、丙二醛(MDA)含量、过氧化氢含量和超氧阴离子含量分别上升了41.6%和32.4%、54.9%和24.4%、58.7%和19.6%、151%和59.8%、94.1%和23.5%、78.9%和47.4%;超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、单脱氢抗坏血酸还原酶(MDHAR)、脱氢抗坏血酸还原酶(DHAR)和谷胱甘肽还原酶(GR)活性分别上升了17.2%和10.9%、57.8%和42.2%、59%和15.7%、32.5%和27%、24.6%和5.7%、36.1%和22.7%、45.5%和33.2%。表明低磷处理能抑制菘蓝幼苗叶片的光合作用,并对叶片造成渗透胁迫,菘蓝幼苗叶片可能通过合成渗透保护物质调节细胞渗透压,并通过抗氧化酶清除活性氧。

Production and mechanical properties of aligned multi-walled carbon nanotubes-M140 composites

This study investigated the production of M140, aligned multi-walled carbon nanotubes (A-MWNTs) reinforced M140 composites (A-MWNTs-M140) and their mechanical properties including their compressive and bending properties as well as their microstructure characteristic of bend fracture surface. M140 was first produced by speed change mixing technics with commercial materials, water-bath curing at normal temperature. In addition, two different A-MWNTs dispersions including carbonyl disper- sions of A-MWNTs (C-A-MWNTs) and aqueous dispersions of A-MWNTs (A-A-MWNTs) with the addition of 0.01wt% A-MWNTs were utilized to obtain enhanced mechanical properties with respect to plain M140. The results indicated that the use of A-MWNTs dispersions allows increasing compressive strength and flexural strength by 8.4% and 5.4%, respectively for the C-A-MWNTs-M140, and by 15.9% and 20.7% for the A-A-MWNTs-M140, respectively. The SEM and EPMA examinations of fracture surface also showed that the bond interface between the nanotubes and matrix is moderate and the main reinforcing mechanisms are microfilling effect, CNTs pull-out and debond. The aqueous dispersion of A-MWNTs is an appropriate method and is more compatible with the M140.