背景:近年研究发现,活化T细胞核因子5(nuclear factor 5 of activated T cells,NFAT5)高表达与多种肿瘤的发生进展关系密切。前期研究发现,NFAT5能够促进人胃癌MGC803细胞增殖并抑制其凋亡。目的:探讨NFAT5对人胃癌MGC803细胞迁移力、...背景:近年研究发现,活化T细胞核因子5(nuclear factor 5 of activated T cells,NFAT5)高表达与多种肿瘤的发生进展关系密切。前期研究发现,NFAT5能够促进人胃癌MGC803细胞增殖并抑制其凋亡。目的:探讨NFAT5对人胃癌MGC803细胞迁移力、侵袭力及细胞周期的影响。方法:实验分为siRNA阴性对照组与NFAT5-siRNA组。有效转染人胃癌MGC803细胞沉默NFAT5基因表达后,采用划痕实验、Transwell实验和流式细胞术分别检测各组细胞迁移力、侵袭力及细胞周期分布的改变。结果与结论:(1)siRNA阴性对照组划痕的愈合能力明显高于NFAT5-siRNA组;(2)NFAT5-siRNA组穿过小室的细胞数目明显少于siRNA阴性对照组[(134.63±25.62)个vs.(195.00±60.41)个,P<0.05];(3)与siRNA阴性对照组相比,NFAT5-siRNA组细胞中G;期细胞比例显著升高[(60.03±0.55)%vs.(62.46±0.73)%,P<0.05],而S期细胞比例显著降低[(28.24±1.16)%vs.(25.44±1.15)%,P<0.05];(4)提示沉默人胃癌MGC803细胞中NFAT5基因表达能够有效减弱细胞的迁移力和侵袭力,改变细胞周期分布,进而减少细胞增殖,NFAT5有望成为胃癌诊治的新靶点。展开更多
The construction of urban healthy environment is the key content of urban planning and landscape design in recent years.Research shows that blue space is closely related to physical and mental health.How to improve th...The construction of urban healthy environment is the key content of urban planning and landscape design in recent years.Research shows that blue space is closely related to physical and mental health.How to improve the ecological and service value of urban blue space of all-aged health through the planning and design of blue space resources is signifi cant to improve urban living environment and physical and mental health of citizens.In this paper,starting from ecology and health,the construction strategies and design points of blue space are discussed through sorting out the relevant research on blue space and health,combined with qualitative case analysis.展开更多
文摘背景:近年研究发现,活化T细胞核因子5(nuclear factor 5 of activated T cells,NFAT5)高表达与多种肿瘤的发生进展关系密切。前期研究发现,NFAT5能够促进人胃癌MGC803细胞增殖并抑制其凋亡。目的:探讨NFAT5对人胃癌MGC803细胞迁移力、侵袭力及细胞周期的影响。方法:实验分为siRNA阴性对照组与NFAT5-siRNA组。有效转染人胃癌MGC803细胞沉默NFAT5基因表达后,采用划痕实验、Transwell实验和流式细胞术分别检测各组细胞迁移力、侵袭力及细胞周期分布的改变。结果与结论:(1)siRNA阴性对照组划痕的愈合能力明显高于NFAT5-siRNA组;(2)NFAT5-siRNA组穿过小室的细胞数目明显少于siRNA阴性对照组[(134.63±25.62)个vs.(195.00±60.41)个,P<0.05];(3)与siRNA阴性对照组相比,NFAT5-siRNA组细胞中G;期细胞比例显著升高[(60.03±0.55)%vs.(62.46±0.73)%,P<0.05],而S期细胞比例显著降低[(28.24±1.16)%vs.(25.44±1.15)%,P<0.05];(4)提示沉默人胃癌MGC803细胞中NFAT5基因表达能够有效减弱细胞的迁移力和侵袭力,改变细胞周期分布,进而减少细胞增殖,NFAT5有望成为胃癌诊治的新靶点。
基金National Nonprofi t Institute Research Grant(DUT19JC55)High-level Talent Innovation Supporting Plan of Dalian City(2017RQ076).
文摘The construction of urban healthy environment is the key content of urban planning and landscape design in recent years.Research shows that blue space is closely related to physical and mental health.How to improve the ecological and service value of urban blue space of all-aged health through the planning and design of blue space resources is signifi cant to improve urban living environment and physical and mental health of citizens.In this paper,starting from ecology and health,the construction strategies and design points of blue space are discussed through sorting out the relevant research on blue space and health,combined with qualitative case analysis.
