Hapalogenys analis(order Lobotiformes)is an economically and ecologically significant fish species.It is a typical sedentary rocky reef fish and is primarily found in the northern Pacific Ocean.Here,we used Hi-C and P...Hapalogenys analis(order Lobotiformes)is an economically and ecologically significant fish species.It is a typical sedentary rocky reef fish and is primarily found in the northern Pacific Ocean.Here,we used Hi-C and PacBio sequencing technique to assemble a high-quality,chromosome-level genome for this species.The 539 Mb genome had a contig N50 with a size of 3.43 Mb,while 755 contigs clustered into 24 chromosomal groups with an anchoring rate of 99.02%.Of the total genomic sequence,132.74Mb(24.39%)were annotated as repeat elements.A total of 21360 protein-coding genes were identified,of which 20787 genes(97.32%)were successfully annotated to public databases.The BUSCO evaluation indicated that 96.90%of the total orthologous genes were matched.The phylogenetic tree representing H.analis and 14 other bony fish species indicated that the H.analis genome contained 364 expanded gene families related to olfactory receptor activity,compared with the common ancestor of H.analis and Sciaenidae.Comparative genomic analysis further identified 3584 contracted gene families.Branch-site modeling identified 277 genes experiencing positive selection,which may facilitate the adaptation to rocky reef environments.The genome reported here is helpful for ecological and evolutionary studies of H.analis.展开更多
临空高超声速飞行器(Near Space Hypersonic Vehicle,NSHV)具备复杂的运动模式和高动态特点,传统的威胁评估方法运用于NSHV时在评估要素选取和评估动态性等方面存在不足。从NSHV的飞行路径入手,将其划分为3个主要的飞行阶段,通过分析其...临空高超声速飞行器(Near Space Hypersonic Vehicle,NSHV)具备复杂的运动模式和高动态特点,传统的威胁评估方法运用于NSHV时在评估要素选取和评估动态性等方面存在不足。从NSHV的飞行路径入手,将其划分为3个主要的飞行阶段,通过分析其各阶段运动特点和预警探测、拦截能力等因素,基于贝叶斯推理、决策理论建立起NSHV多阶段威胁评估模型,并通过先验概率将各阶段进行关联,保证了评估的继承性,最终建立典型场景进行仿真验证,仿真结果反映的威胁变化符合NSHV的作战特点和所给观测数据,证明该方法更适应NSHV的动态特性,能够为指挥员进行防御作战辅助决策提供支持。展开更多
Black rockfish Sebastes schlegelii juveniles （30-40 mm total length） were immersed in a range of calcein （CAL） solutions at concentrations ranging from 50 to 250 mg/L and alizarin red S （ARS） solutions at concen...Black rockfish Sebastes schlegelii juveniles （30-40 mm total length） were immersed in a range of calcein （CAL） solutions at concentrations ranging from 50 to 250 mg/L and alizarin red S （ARS） solutions at concentrations ranging from 100 to 500 mg/L in filtered seawater （salinity 30） for 24 h. Fluorescent marks were detected in otoliths （sagittae, asteriscus）, scales, fin rays （dorsal, pectoral, ventral, anal, and caudal fin rays）, and fin spines （dorsal, ventral, and anal fin spines） after a 60-d growth experiment. With the exception of 50-100 rng/L CAL, acceptable marks were produced in the otoliths and fin spines by all concentrations of CAL and ARS. In particular, marks were clearly visible under normal light in the sagittae, asteriscus, and fin spines offish immersed in 200 500 mg/L, 300-500 rag/L, and 200-500 mg/LARS, respectively. Scales and fin rays had acceptable marks at much higher concentrations （≥50 mg/L CAL, ≥300 mg/L ARS for scales and ≥50 mg/L CAL,≥200 mg/L ARS for fin rays）. The mark quality was highest （i.e., acceptable marks were observed in all sampled structures after immersion marking） in fish immersed in 150-250 mg/L CAL or 300-500 mg/LARS. In addition, there was no significant difference in survival and growth of marked fish compared with controls 60 d post-marking （P〉0.05）.展开更多
Simple sequence repeat （SSR） markers were obtained for the large yellow croaker Pseudosciaena crocea using 1 205 expressed sequences tags （ESTs） from the NCBI database.Primers for 48 ESTSSR loci were designed and ...Simple sequence repeat （SSR） markers were obtained for the large yellow croaker Pseudosciaena crocea using 1 205 expressed sequences tags （ESTs） from the NCBI database.Primers for 48 ESTSSR loci were designed and screened with 30 P.crocea specimens captured from Guanjingyang sea area in Fujian Province of China.Sixteen of the loci were polymorphic,which were amplified with 3 to 11 alleles per locus and the mean of 6.13.The observed and expected heterozygosity per locus ranged from 0.091 to 0.844 （mean 0.544） and from 0.118 to 0.892 （mean 0.644）,respectively.Polymorphic information content （PIC） ranged from 0.115 to 0.866 （mean 0.593）.The results for cross-species amplification of the 16 large yellow croaker EST-SSRs on P.polyactis,C.niveatus,C.lucidus,A.argentatus and J.belengeri revealed that 14,12,11,7 and 6 loci were successfully amplified with 1 to 10 alleles with an average of 4.5 per locus,respectively,which are suitable for population genetics studies of these species and useful for phylogenetic relationship analysis among these species.Overall,this study provides a set of type I markers for population genetics studies and genome mapping for large yellow croaker and its closely related species.展开更多
The digestive tract of Takifugu rubripes during early life stages was studied with light microscopy. At the beginning of hatching, the digestive tract is represented by a simple and undifferentiated straight tube and ...The digestive tract of Takifugu rubripes during early life stages was studied with light microscopy. At the beginning of hatching, the digestive tract is represented by a simple and undifferentiated straight tube and does not communicate with the exterior, as the mouth and anus are not open yet. At 2 d after hatching, a constriction between intestine and rectum that will become the intestino-rectal valve is visible. During the endogenous feeding phase, the yolk sac is resorbed and the digestive tract becomes functional and differentiated. The liver and pancreas also become apparent at this time. At onset of exogenous feeding （3 d after hatching）, yolk sac resesves are not completely depleted, suggesting a period of mixed nutrition. The digestive tract differentiates fully into b.uccopharynx, esophagus, stomach, intestine and rectum. At 9 d after hatching, the yolk sac reserves are completely depleted. The most noticeable events occurring from 5 to 17 d after hatching are the transformation of the epithelia type, the differentiation of the pneumatic sac, the epithelial cell, gut convolution, mucosal fold increase, and the growth of liver and pancreas. From 18 to 27 d after hatching, the numbers of intestine folds and mucus cells increase progressively. From then on, morphological changes of digestive features are almost completed. At 27 d after hatching, the morphology and function of digestive system are similar to that of the adult.展开更多
基金supported by the Province Key Research and Development Program of Zhejiang (No.2021C02047)the Special Projects of Zhejiang Provincial Science and Technology Department (Nos.HYS-CZ-004,HYS-CZ-202208)the‘San Nong Jiu Fang’Science and Technology Cooperation Project of Zhejiang Province (No.2022 SN JF073)。
文摘Hapalogenys analis(order Lobotiformes)is an economically and ecologically significant fish species.It is a typical sedentary rocky reef fish and is primarily found in the northern Pacific Ocean.Here,we used Hi-C and PacBio sequencing technique to assemble a high-quality,chromosome-level genome for this species.The 539 Mb genome had a contig N50 with a size of 3.43 Mb,while 755 contigs clustered into 24 chromosomal groups with an anchoring rate of 99.02%.Of the total genomic sequence,132.74Mb(24.39%)were annotated as repeat elements.A total of 21360 protein-coding genes were identified,of which 20787 genes(97.32%)were successfully annotated to public databases.The BUSCO evaluation indicated that 96.90%of the total orthologous genes were matched.The phylogenetic tree representing H.analis and 14 other bony fish species indicated that the H.analis genome contained 364 expanded gene families related to olfactory receptor activity,compared with the common ancestor of H.analis and Sciaenidae.Comparative genomic analysis further identified 3584 contracted gene families.Branch-site modeling identified 277 genes experiencing positive selection,which may facilitate the adaptation to rocky reef environments.The genome reported here is helpful for ecological and evolutionary studies of H.analis.
文摘临空高超声速飞行器(Near Space Hypersonic Vehicle,NSHV)具备复杂的运动模式和高动态特点,传统的威胁评估方法运用于NSHV时在评估要素选取和评估动态性等方面存在不足。从NSHV的飞行路径入手,将其划分为3个主要的飞行阶段,通过分析其各阶段运动特点和预警探测、拦截能力等因素,基于贝叶斯推理、决策理论建立起NSHV多阶段威胁评估模型,并通过先验概率将各阶段进行关联,保证了评估的继承性,最终建立典型场景进行仿真验证,仿真结果反映的威胁变化符合NSHV的作战特点和所给观测数据,证明该方法更适应NSHV的动态特性,能够为指挥员进行防御作战辅助决策提供支持。
基金Supported by the Special Fund for Agro-scientific Research in the Public Interest(No.201003068)the National Natural Science Foundation of China(Nos.31172447,41176117)
文摘Black rockfish Sebastes schlegelii juveniles （30-40 mm total length） were immersed in a range of calcein （CAL） solutions at concentrations ranging from 50 to 250 mg/L and alizarin red S （ARS） solutions at concentrations ranging from 100 to 500 mg/L in filtered seawater （salinity 30） for 24 h. Fluorescent marks were detected in otoliths （sagittae, asteriscus）, scales, fin rays （dorsal, pectoral, ventral, anal, and caudal fin rays）, and fin spines （dorsal, ventral, and anal fin spines） after a 60-d growth experiment. With the exception of 50-100 rng/L CAL, acceptable marks were produced in the otoliths and fin spines by all concentrations of CAL and ARS. In particular, marks were clearly visible under normal light in the sagittae, asteriscus, and fin spines offish immersed in 200 500 mg/L, 300-500 rag/L, and 200-500 mg/LARS, respectively. Scales and fin rays had acceptable marks at much higher concentrations （≥50 mg/L CAL, ≥300 mg/L ARS for scales and ≥50 mg/L CAL,≥200 mg/L ARS for fin rays）. The mark quality was highest （i.e., acceptable marks were observed in all sampled structures after immersion marking） in fish immersed in 150-250 mg/L CAL or 300-500 mg/LARS. In addition, there was no significant difference in survival and growth of marked fish compared with controls 60 d post-marking （P〉0.05）.
基金The National High Technology Research and Development Program of China (863 program) under contract Nos 2006AA10A405 and 2006AA09Z418the National Natural Science Foundation of China under contract Nos 30771663 and 30471329the Foundation for Innovation Research Team of Jimei University,China under contract No.2006A001
文摘Simple sequence repeat （SSR） markers were obtained for the large yellow croaker Pseudosciaena crocea using 1 205 expressed sequences tags （ESTs） from the NCBI database.Primers for 48 ESTSSR loci were designed and screened with 30 P.crocea specimens captured from Guanjingyang sea area in Fujian Province of China.Sixteen of the loci were polymorphic,which were amplified with 3 to 11 alleles per locus and the mean of 6.13.The observed and expected heterozygosity per locus ranged from 0.091 to 0.844 （mean 0.544） and from 0.118 to 0.892 （mean 0.644）,respectively.Polymorphic information content （PIC） ranged from 0.115 to 0.866 （mean 0.593）.The results for cross-species amplification of the 16 large yellow croaker EST-SSRs on P.polyactis,C.niveatus,C.lucidus,A.argentatus and J.belengeri revealed that 14,12,11,7 and 6 loci were successfully amplified with 1 to 10 alleles with an average of 4.5 per locus,respectively,which are suitable for population genetics studies of these species and useful for phylogenetic relationship analysis among these species.Overall,this study provides a set of type I markers for population genetics studies and genome mapping for large yellow croaker and its closely related species.
文摘The digestive tract of Takifugu rubripes during early life stages was studied with light microscopy. At the beginning of hatching, the digestive tract is represented by a simple and undifferentiated straight tube and does not communicate with the exterior, as the mouth and anus are not open yet. At 2 d after hatching, a constriction between intestine and rectum that will become the intestino-rectal valve is visible. During the endogenous feeding phase, the yolk sac is resorbed and the digestive tract becomes functional and differentiated. The liver and pancreas also become apparent at this time. At onset of exogenous feeding （3 d after hatching）, yolk sac resesves are not completely depleted, suggesting a period of mixed nutrition. The digestive tract differentiates fully into b.uccopharynx, esophagus, stomach, intestine and rectum. At 9 d after hatching, the yolk sac reserves are completely depleted. The most noticeable events occurring from 5 to 17 d after hatching are the transformation of the epithelia type, the differentiation of the pneumatic sac, the epithelial cell, gut convolution, mucosal fold increase, and the growth of liver and pancreas. From 18 to 27 d after hatching, the numbers of intestine folds and mucus cells increase progressively. From then on, morphological changes of digestive features are almost completed. At 27 d after hatching, the morphology and function of digestive system are similar to that of the adult.
