在基础实验教学中,氢氧化铁胶体的制备及电泳实验,失败率居高不下。论文探讨了导致实验失败的可能因素,改进了胶体的纯化条件,优化了FeCl_3的用量,提高实验成功率。0.3或0.5 g FeCl_3固体制备约200 mL Fe(OH)_3胶体,在磁力搅拌下,用购...在基础实验教学中,氢氧化铁胶体的制备及电泳实验,失败率居高不下。论文探讨了导致实验失败的可能因素,改进了胶体的纯化条件,优化了FeCl_3的用量,提高实验成功率。0.3或0.5 g FeCl_3固体制备约200 mL Fe(OH)_3胶体,在磁力搅拌下,用购买的透析袋装130 mL胶体,50℃的蒸馏水透析,10 min换水,透析5次。透析后的胶体电泳现象明显,界面清晰,计算的ζ值与文献值更接近。展开更多
目的:建立HPLC-QAMS(high performance liquid chromatography-quantitative analysis of multi-components by single marker)法同时测定癫痫康胶囊中天麻素、巴利森苷E、巴利森苷B、巴利森苷、细叶远志皂苷、远志皂苷B、麦冬甲基黄烷...目的:建立HPLC-QAMS(high performance liquid chromatography-quantitative analysis of multi-components by single marker)法同时测定癫痫康胶囊中天麻素、巴利森苷E、巴利森苷B、巴利森苷、细叶远志皂苷、远志皂苷B、麦冬甲基黄烷酮A、甲基麦冬二氢高异黄酮B、β-细辛醚和α-细辛醚含量的方法。方法:应用Waters Symmetry C18色谱柱,乙腈-0.05%磷酸溶液为流动相进行梯度洗脱。多波长切换检测。以细叶远志皂苷为含量测定的内参物,通过相对校正因子对天麻素、巴利森苷E、巴利森苷B、巴利森苷、远志皂苷B、麦冬甲基黄烷酮A、甲基麦冬二氢高异黄酮B、β-细辛醚和α-细辛醚的含量进行计算。同时采用外标法(external standard method,ESM)测定12批癫痫康胶囊中10种成分含量。结果:建立了以细叶远志皂苷为内参比物质的HPLC-QAMS方法,天麻素、巴利森苷E、巴利森苷B、巴利森苷、远志皂苷B、麦冬甲基黄烷酮A、甲基麦冬二氢高异黄酮B、β-细辛醚和α-细辛醚的相对校正因子分别为0.8238、0.7239、1.0229、1.1881、0.7272、1.3108、0.9314、0.6549和1.0572,相对校正因子重复性良好,与ESM无显著性差异(P>0.05)。结论:HPLC-QAMS法可用于癫痫康胶囊中多指标成分含量的同时测定。展开更多
Objective:To establish an high performance liquid chromatography-quantitative analysis of multi components by single marker(HPLC-QAMS)method for simultaneous determination of gastrodin,parishin E,parishin B,parishin,t...Objective:To establish an high performance liquid chromatography-quantitative analysis of multi components by single marker(HPLC-QAMS)method for simultaneous determination of gastrodin,parishin E,parishin B,parishin,tenuifolin,onjisaponin B,methylophiopogonanone A,methylophiopogonanone B,β-asarone andαasarone in Dianxiankang Capsules.Methods:Waters Symmetry C_(18)column was used with acetonitrile-0.05%phosphoric acid solution as mobile phase for gradient elution.Multiwavelength switching detection.The contents of gastrodin,parishin E,parishin B,parishin,onjisaponin B,methylophiopogonanone A,methylophiopogonanone B,β-asarone andα-asarone were calculated by relative correction factor.At the same time,the contents of 10 components in 12 batches of Dianxiankang Capsules were determined by external standard method(ESM).Results:An HPLC-QAMS method was established tenuifolin as the internal reference substance was established.The relative correction factors of gastrodin,parishin E,parishin B,parishin,onjisaponin B,methylophiopogonanone A,methylophiopogonanone B,β-asarone andα-asarone were 0.8238,0.7239,1.0229,1.1881,0.7272,1.3108,0.9314,0.6549 and 1.0572,respectively.The relative correction factors had good repeatability and no significant difference with ESM(P>0.05).Conclusion:HPLC-QAMS can be used for simultaneous determination of multi-index components in Dianxiankang Capsules.展开更多
文摘在基础实验教学中,氢氧化铁胶体的制备及电泳实验,失败率居高不下。论文探讨了导致实验失败的可能因素,改进了胶体的纯化条件,优化了FeCl_3的用量,提高实验成功率。0.3或0.5 g FeCl_3固体制备约200 mL Fe(OH)_3胶体,在磁力搅拌下,用购买的透析袋装130 mL胶体,50℃的蒸馏水透析,10 min换水,透析5次。透析后的胶体电泳现象明显,界面清晰,计算的ζ值与文献值更接近。
文摘目的:建立HPLC-QAMS(high performance liquid chromatography-quantitative analysis of multi-components by single marker)法同时测定癫痫康胶囊中天麻素、巴利森苷E、巴利森苷B、巴利森苷、细叶远志皂苷、远志皂苷B、麦冬甲基黄烷酮A、甲基麦冬二氢高异黄酮B、β-细辛醚和α-细辛醚含量的方法。方法:应用Waters Symmetry C18色谱柱,乙腈-0.05%磷酸溶液为流动相进行梯度洗脱。多波长切换检测。以细叶远志皂苷为含量测定的内参物,通过相对校正因子对天麻素、巴利森苷E、巴利森苷B、巴利森苷、远志皂苷B、麦冬甲基黄烷酮A、甲基麦冬二氢高异黄酮B、β-细辛醚和α-细辛醚的含量进行计算。同时采用外标法(external standard method,ESM)测定12批癫痫康胶囊中10种成分含量。结果:建立了以细叶远志皂苷为内参比物质的HPLC-QAMS方法,天麻素、巴利森苷E、巴利森苷B、巴利森苷、远志皂苷B、麦冬甲基黄烷酮A、甲基麦冬二氢高异黄酮B、β-细辛醚和α-细辛醚的相对校正因子分别为0.8238、0.7239、1.0229、1.1881、0.7272、1.3108、0.9314、0.6549和1.0572,相对校正因子重复性良好,与ESM无显著性差异(P>0.05)。结论:HPLC-QAMS法可用于癫痫康胶囊中多指标成分含量的同时测定。
基金National Traditional Chinese Medicine Technology Inheritance Talent Training Project(NO.T20194828003)。
文摘Objective:To establish an high performance liquid chromatography-quantitative analysis of multi components by single marker(HPLC-QAMS)method for simultaneous determination of gastrodin,parishin E,parishin B,parishin,tenuifolin,onjisaponin B,methylophiopogonanone A,methylophiopogonanone B,β-asarone andαasarone in Dianxiankang Capsules.Methods:Waters Symmetry C_(18)column was used with acetonitrile-0.05%phosphoric acid solution as mobile phase for gradient elution.Multiwavelength switching detection.The contents of gastrodin,parishin E,parishin B,parishin,onjisaponin B,methylophiopogonanone A,methylophiopogonanone B,β-asarone andα-asarone were calculated by relative correction factor.At the same time,the contents of 10 components in 12 batches of Dianxiankang Capsules were determined by external standard method(ESM).Results:An HPLC-QAMS method was established tenuifolin as the internal reference substance was established.The relative correction factors of gastrodin,parishin E,parishin B,parishin,onjisaponin B,methylophiopogonanone A,methylophiopogonanone B,β-asarone andα-asarone were 0.8238,0.7239,1.0229,1.1881,0.7272,1.3108,0.9314,0.6549 and 1.0572,respectively.The relative correction factors had good repeatability and no significant difference with ESM(P>0.05).Conclusion:HPLC-QAMS can be used for simultaneous determination of multi-index components in Dianxiankang Capsules.