In order to chitracterizc the speclrum of mutallon indueed by glyeidyl methacrylule(GMA ), the plasmid pBR322 was modificed with this mutagen in vitro , transttclcd intoapproprate Escherichia colii host HB101. Th...In order to chitracterizc the speclrum of mutallon indueed by glyeidyl methacrylule(GMA ), the plasmid pBR322 was modificed with this mutagen in vitro , transttclcd intoapproprate Escherichia colii host HB101. The mutants were then sereened and defined by DNA sequencing . Sequence analysis reveals that G MA induces two classes of mulations deletion of the mono-. di' or fetra-base or the insertion of mono' or di-base. Both typesof mutations, with about 10 % frequency. occur predominantly at C-G runs and at5'-CNCCN-3' sequence ,which are hotspots for GMA damage and may cause frameshift mutation.展开更多
文摘In order to chitracterizc the speclrum of mutallon indueed by glyeidyl methacrylule(GMA ), the plasmid pBR322 was modificed with this mutagen in vitro , transttclcd intoapproprate Escherichia colii host HB101. The mutants were then sereened and defined by DNA sequencing . Sequence analysis reveals that G MA induces two classes of mulations deletion of the mono-. di' or fetra-base or the insertion of mono' or di-base. Both typesof mutations, with about 10 % frequency. occur predominantly at C-G runs and at5'-CNCCN-3' sequence ,which are hotspots for GMA damage and may cause frameshift mutation.
