The influence of OCT1 gene polymorphisms on the metformin response in Uygur patients with glucose metabolism disorder

Objective To determine the effects of genetic variation in the organic cation transporter 1(OCT1)on the short-term responses of the antidiabetic drug,metformin.Method A total of 22 patients recruited with type 2 diabetes or IFG were treated with metformin(2 000 mg/day)for 1 week.The patients were screened from Second Jikun hospital and Kashidonglu community medicine service,Urumqi,China and their surrounding districts.To examine the effects of metformin on plasma glucose,total cholesterol,low-density lipoprotein-cholesterol,high-density lipoprotein-cholesterol and triglyceride in relation with R61C,G465R and 420 del variants of OCT1(gene encoding organic cation transporter 1,mainly locating in liver,which is metformin's major target)in subjects.In all,R61C,G465R and 420del of OCT1 gene were examined using DNA extracted from whole blood and PCR-RFLP.Data concerning with gene and metformin treatment were handled by t-test.Result After metformin treatment,there were increases both in FPG and LDL(P=0.011and P=0.013 respectively).To divide all participants into mutant and wild groups,according to the polymorphisms of R61C,G465R and 420 del respectively,as well as carriers with one of the mutant genotypes at least and carriers with none of the mutant sites.Analysis was made to compared FPG,Chol,TG,and LDL and HDL between carriers of wild genotypes and carriers of other genotypes showed no statistic significance both before the metformin treatment and after the treatment.The same is the case with changes of FPG,Chol,TG,and LDL and HDL of wild genotype carriers and variant genotype carriers,except of LDL changes(P=0.05)in patients grouped by G465R polymorphisms and TG changes(P=0.03)in subjects differed by 420del genotypes.Conclusion In this study,it is suggested that OCT1 gene polymorphisms have little contribution to the clinical efficacy of blood glucose control by metformin among Uygur people with type 2 diabetes or IFG,but it may have possible relationship with the clinical efficacy on fat metabolism by metformin.

超声引导下远端收肌管阻滞在胫骨骨折患者术后镇痛中的应用效果观察

目的:观察超声引导下远端收肌管阻滞在胫骨骨折患者术后镇痛中的应用效果。方法:选择2019年8月-2021年12月大理市第一人民医院收治的80例胫骨骨折患者作为研究对象,随机分为研究组与对照组,各40例。研究组实施超声引导下远端收肌管阻滞,对照组实施静脉病人自控镇痛。比较两组住院时间、自控镇痛按压次数、不良反应发生率以及镇痛后1 h、12 h、24 h的视觉模拟评分法(VAS)评分。结果:研究组住院时间短于对照组,自控镇痛按压次数少于对照组,差异有统计学意义(P<0.05)。研究组不良反应发生率低于对照组,差异有统计学意义(P<0.05)。镇痛后1 h、12 h、24 h,研究组VAS评分均低于对照组,差异有统计学意义(P<0.05)。结论:超声引导下远端收肌管阻滞对胫骨骨折术后患者的镇痛效果较好,可缩短患者住院时间,减少不良反应的发生,提高患者围术期的舒适度。