A simple sensitive spectrofluorimetric method was developed for the determination of amoxicillin. Amoxicillin in the presence of formaldehyde produces a fluorescent pyrazine derivative, whose fluorescence intensity ca...A simple sensitive spectrofluorimetric method was developed for the determination of amoxicillin. Amoxicillin in the presence of formaldehyde produces a fluorescent pyrazine derivative, whose fluorescence intensity can be enhanced by forming an inclusion complex with β-cyclodextrin. Optimum conditions for the determination of amoxicillin were also investigated. Fluorimetric detection was carried out at λex=365 nm and λem=445 nm. The linear range of this method for the determination of amoxicillin is 0.1―10 mg/L and the limit of detection is 0.03 mg/L （IF=102.16ρ-9.6, r=0.9994）. The developed method is practical and can be successfully applied to determine the content of amoxicillin in capsules and tablets with satisfactory results.展开更多
基金Supported by the National Natural Science Foundation of China(No.40973081)the Foundation for Analysis and Measurement of Kunming University of Science and Technology+2 种基金 China(No.2009092)the Innovation Fund for Small Technology Based Firms China (No.10C26215305131)
文摘A simple sensitive spectrofluorimetric method was developed for the determination of amoxicillin. Amoxicillin in the presence of formaldehyde produces a fluorescent pyrazine derivative, whose fluorescence intensity can be enhanced by forming an inclusion complex with β-cyclodextrin. Optimum conditions for the determination of amoxicillin were also investigated. Fluorimetric detection was carried out at λex=365 nm and λem=445 nm. The linear range of this method for the determination of amoxicillin is 0.1―10 mg/L and the limit of detection is 0.03 mg/L （IF=102.16ρ-9.6, r=0.9994）. The developed method is practical and can be successfully applied to determine the content of amoxicillin in capsules and tablets with satisfactory results.
