We report a general strategy to develop injectable welded nanofibers to facilitate the outgrowth and extension of neurites.In this case,nonwoven mats of uniaxially aligned poly(caprolactone)(PCL)nanofibers were firstl...We report a general strategy to develop injectable welded nanofibers to facilitate the outgrowth and extension of neurites.In this case,nonwoven mats of uniaxially aligned poly(caprolactone)(PCL)nanofibers were firstly cut into several small pieces with fixed fiber lengths of 25,50 and 100µm,respectively,using a cryotome.A tissuelyser was employed to homogenize and disperse the short nanofibers to a homogeneous suspension.By tuning treatment duration from 100 s to 400 s,the temperature of the suspension was brought close to the melting point of PCL.As such,the short nanofibers were welded at their cross points while the fibers far away from the cross points remain the original structures.We showed that the viability of neuroblastoma SH-SY5Y cells and their neurite outgrowth and extension were enhanced with the use of such welded short nanofibers.Taken together,this study provides a simple way to generate injectable welded nanofibers,holding potential in affecting neurite outgrowth and extension for nerve repair,in particular,in the central nervous system.展开更多
基金This work was supported by the National Natural Science Foundation of China(No.82001970)the Startup Funds from Qingdao University,China(No.DC2000002357).
文摘We report a general strategy to develop injectable welded nanofibers to facilitate the outgrowth and extension of neurites.In this case,nonwoven mats of uniaxially aligned poly(caprolactone)(PCL)nanofibers were firstly cut into several small pieces with fixed fiber lengths of 25,50 and 100µm,respectively,using a cryotome.A tissuelyser was employed to homogenize and disperse the short nanofibers to a homogeneous suspension.By tuning treatment duration from 100 s to 400 s,the temperature of the suspension was brought close to the melting point of PCL.As such,the short nanofibers were welded at their cross points while the fibers far away from the cross points remain the original structures.We showed that the viability of neuroblastoma SH-SY5Y cells and their neurite outgrowth and extension were enhanced with the use of such welded short nanofibers.Taken together,this study provides a simple way to generate injectable welded nanofibers,holding potential in affecting neurite outgrowth and extension for nerve repair,in particular,in the central nervous system.
