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miR-486-5p靶向FOXO1抑制结肠癌干细胞干性的初步研究 被引量:4
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作者 龚立刚 艾成思 王梦萍 《安徽医科大学学报》 CAS 北大核心 2020年第4期528-533,共6页
目的研究miR-486-5p在结肠癌(CRC)细胞系和CRC干细胞(CSCs)中的表达水平,并探讨miR-486-5p对CRC CSCs干性的影响和对FOXO1的靶向调控作用。方法qRT-PCR检测miR-486-5p在CRC细胞系SW480、HCT116、SW620和HT29及正常结肠细胞系NCM460中的... 目的研究miR-486-5p在结肠癌(CRC)细胞系和CRC干细胞(CSCs)中的表达水平,并探讨miR-486-5p对CRC CSCs干性的影响和对FOXO1的靶向调控作用。方法qRT-PCR检测miR-486-5p在CRC细胞系SW480、HCT116、SW620和HT29及正常结肠细胞系NCM460中的表达水平。培养HCT116细胞中的CSCs。Western blot检测Nanog、SOX2、OCT4干性标志蛋白及miR-486-5p在CSCs中的表达。经脂质体分别转染miR-486-5p mimic和对照(NC)48 h后,MTS检测CSCs增殖能力,Transwell实验检测CSCs转移能力,Boyden实验检测CSCs侵袭能力。遗传霉素(G418)筛选稳定过表达miR-486-5p的CSCs细胞,注射到BALB/c裸鼠皮下,观察miR-486-5p对CSCs体内致瘤能力的影响。经Targetscan 7. 1软件预测及双荧光素酶报道基因实验,验证miR-486-5p对FOXO1基因的靶向作用,qRT-PCR检测miR-486-5p对CSCs中FOXO1 mRNA表达影响。结果qRT-PCR结果显示miR-486-5p在CRC细胞系中的表达低于正常结肠细胞系(均P <0. 05)。OCT4、Nanog、SOX2干细胞标志物在CSCs中表达上调;miR-486-5p在CSCs中表达显著下降;miR-486-5p mimic后,CSCs细胞增殖、转移及侵袭能力均下降(均P <0. 05)。CSCsm iR-486-5p裸鼠成瘤能力降低(P<0. 05)。Target Scan7. 1预测软件和双荧光素酶报道基因实验证实FOXO1为miR-486-5p的靶基因,miR-486-5p mimic组FOXO1 mRNA的表达减少(P <0. 05)。结论 miR-486-5p在CRC细胞系和CSCs中均低表达,miR-486-5p能通过负调控FOXO1抑制CSCs的干性。 展开更多
关键词 结肠癌 CSCs miR-486-5p FOXO1 干性
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Switching disturbance rejection attitude control of near space vehicles with variable structure 被引量:2
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作者 gong ligang WANG Qing DONG Chaoyang 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2019年第1期167-179,共13页
A switching disturbance rejection attitude control law is proposed for a near space vehicle(NSV) with variable structure.The multiple flight modes, system uncertainties and disturbances of the NSV are taken into accou... A switching disturbance rejection attitude control law is proposed for a near space vehicle(NSV) with variable structure.The multiple flight modes, system uncertainties and disturbances of the NSV are taken into account based on switched nonlinear systems. Compared with traditional backstepping design methods,the proposed method utilizes the added integrals of attitude angle and angular rate tracking errors to further decrease the tracking errors. Moreover, to reduce the computation complexity, a rapid convergent differentiator is employed to obtain the derivative of the virtual control command. Finally, for disturbance rejection, based on the idea from the extended state observer(ESO), two disturbance observers are designed by using non-smooth functions to estimate the disturbances in the switched nonlinear systems. All signals of the closed-loop system are proven to be uniformly ultimately bounded under the Lyapunov function framework. Simulation results demonstrate the effectiveness of the proposed control scheme. 展开更多
关键词 NEAR space vehicle (NSV) variable structure SWITCHED nonlinear system extended state OBSERVER (ESO).
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沉默STC2对裸鼠结肠癌模型中肝转移瘤形成情况及IL-10和VEGF表达水平的影响 被引量:3
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作者 龚立刚 王梦萍 +2 位作者 刘文俊 李菲 任斌 《结直肠肛门外科》 2018年第3期217-222,共6页
目的建立结肠癌肝转移裸鼠模型,探讨斯钙素2(Stanniocalcin 2,STC2)在结肠癌肝转移中的作用。方法利用慢病毒载体将shRNA转染入HCT116细胞以沉默STC2,构建沉默STC2的HCT116细胞系,并用Western Blot验证沉默STC2效果,建立结肠癌肝转移动... 目的建立结肠癌肝转移裸鼠模型,探讨斯钙素2(Stanniocalcin 2,STC2)在结肠癌肝转移中的作用。方法利用慢病毒载体将shRNA转染入HCT116细胞以沉默STC2,构建沉默STC2的HCT116细胞系,并用Western Blot验证沉默STC2效果,建立结肠癌肝转移动物模型,分析STC2在结肠癌肝转移中的作用。将24只雄性Balb/c裸鼠随机分为实验组及对照组,每组12只。实验组将沉默STC2的HCT116细胞系—HCT116p LL3.7-STC2注射入Balb/c裸鼠脾脏并保留脾脏建模;对照组将未沉默STC2的HCT116细胞系—HCT116p LL3.7-Con注射入Balb/c裸鼠脾脏并保留脾脏建模。观察两组Balb/c裸鼠肝转移瘤形成情况,采用免疫组织化学方法检测Balb/c裸鼠肝脏内转移瘤中IL-10和VEGF的表达。结果 Western Blot实验提示造模成功,沉默STC2的实验组细胞HCT116p LL3.7-STC2中STC2蛋白量的表达少于对照组细胞HCT116p LL3.7-Con。实验组肝转移瘤发生率为41.67%(5/12),对照组为91.67%(11/12),组间差异有统计学意义(P<0.05)。实验组肝转移瘤数量少于对照组(P<0.05),两组肝转移瘤大小和肝脏重量的差异均无统计学意义(均P>0.05)。实验组肝转移瘤中IL-10及VEGF表达水平均低于对照组(均P<0.05)。结论在裸鼠模型中,将STC2沉默后的结肠癌细胞肝转移潜能下降,且其肝转移瘤中IL-10及VEGF的表达水平低于未沉默者。 展开更多
关键词 斯钙素2 结肠癌肝转移 裸鼠模型
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Closed-loop fault detection for full-envelope flight vehicle with measurement delays 被引量:3
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作者 Wang Zhaolei Wang Qing +1 位作者 Dong Chaoyang gong ligang 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2015年第3期832-844,共13页
Abstract A closed-loop fault detection problem is investigated for the full-envelope flight vehicle with measurement delays, where the flight dynamics are modeled as a switched system with delayed feedback signals. Th... Abstract A closed-loop fault detection problem is investigated for the full-envelope flight vehicle with measurement delays, where the flight dynamics are modeled as a switched system with delayed feedback signals. The mode-dependent observer-based fault detection filters and state estimation feedback controllers are derived by considering the delays' impact on the control system and fault detection system simultaneously. Then, considering updating lags of the controllers/filters' switching signals which are introduced by the delayed measurement of altitude and Mach number, an asynchronous H analysis method is proposed and the system model is further augmented to be an asynchronously switched time-delay system. Also, the global stability and desired performance of the augmented system are guaranteed by combining the switched delay-dependent Lyapunov Krasovskii functional method with the average dwell time method (ADT), and the delaydependent existing conditions for the controllers and fault detection filters are obtained in the form of the linear matrix inequalities (LMIs), Finally, numerical example based on the hypersonic vehicles and highly maneuverable technology (HiMAT) vehicle is given to demonstrate the merits of the proposed method. 展开更多
关键词 Fault diagnosis Measurement delays Asynchronous switching Full-envelope flight vehicle Closed-loop fault detection
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