目的探讨静脉性耳鸣患者蛛网膜颗粒内脑疝(brain herniation into arachnoid granulation,BHAG)对于横窦跨狭窄压力梯度(tra ns-stenostic pressure gradient,TPG)升高(≥8m m H g)的诊断效能。方法回顾性分析2010年11月至2023年10月于...目的探讨静脉性耳鸣患者蛛网膜颗粒内脑疝(brain herniation into arachnoid granulation,BHAG)对于横窦跨狭窄压力梯度(tra ns-stenostic pressure gradient,TPG)升高(≥8m m H g)的诊断效能。方法回顾性分析2010年11月至2023年10月于首都医科大学附属北京友谊医院住院的静脉性耳鸣患者临床及影像学资料,所有患者均行静脉窦非增强M RV(non-contrast enhanced MR venography,NCE-MRV)、全脑MPRAGE及3D-T_(2)WI扫描,并经DSA行静脉窦测压,将患者分为A(TPG<8 mm Hg)、B(TPG≥8 mm Hg)两组。结合全脑MPRAGF及3D-T_(2)WI的标准冠状面图像,评价是否存在BHAG。对A、B两组BHAG发生率是否存在差异进行卡方检验;计算BHAG对于诊断高TPG(≥8 mm Hg)特异度、灵敏度、准确度、阳性预测值及阴性预测值。结果共73例患者符合纳排标准(男性9例,女性64例),年龄37.0(46.5-30.0)岁,其中A组(TPG<8 mm Hg)46例,B组(TPG≥8 mm Hg)27例。BHAG患者共12例(12/73,16.44%),A、B两组BHAG发生率存在统计学差异(χ^(2)值=10.96,P<0.001)。BHAG诊断高TPG(≥8mmHg)的特异度为95.65%、灵敏度为37.04%、准确度为73.97%、阳性预测值为83.33%,阴性预测值为72.13%。结论BHAG在静脉性耳鸣患者中并不罕见,可用于辅助预估有无TPG升高。展开更多
Background The relationship between apoptosis and tumors is a major focus in cancer research. RNA interference （RNAi） technology has emerged as a very potent tool to generate cellular knockdown phenotypes of a desi...Background The relationship between apoptosis and tumors is a major focus in cancer research. RNA interference （RNAi） technology has emerged as a very potent tool to generate cellular knockdown phenotypes of a desired gene. The aim of this study was to explore the effect of siRNA specific to the protein casein kinase 2α （CK2α） on apoptosis of laryngeal carcinoma cells and to explore possible mechanisms.Methods An siRNA expression plasmid specific to CK2α, psiRNA-hH1neo-CK2α, and a non-specific siRNA expression plasmid, psiRNA-hH1neo-cont, were constructed and transfected into Hep-2 cells by a lipofectamine method. The mRNA and protein levels of CK2α in transfected cells were determined by reverse transcription-polymerase chain reaction （RT-PCR） and Western blotting analysis. Apoptosis was measured by Annexin V-fluorescein isothiocyanate （FITC）/ propidium iodide （PI） double-staining methods. The morphological changes to Hep-2 cells were observed under transmission electron microscopy （TEM）. The levels of Bcl-2 and Bax proteins were measured by Western blotting analysis.Results Levels of CK2α mRNA and protein were significantly decreased in the psiRNA-hH1neo-CK2α group compared to the other groups （P 〈0.05）. The apoptotic rate of the psiRNA-hH1neo-CK2α transfected group was significantly higher compared to that in the untransfected group and the siRNA-hH1neo-cont transfected group （25.66%±0.83%, 3.66%±0.43%, and 5.18%±0.22%） （P 〈0.05）. Compared with the untransfected group and the siRNA-hH1neo-cont transfected group, the psiRNA-hH1neo-CK2α transfected group presented with classical ultrastructural features of apoptosis, such as karyopyknosis, chromatic agglutination adjacent to the nuclear membrane, and apoptotic bodies. Compared with the other two groups, the level of Bcl-2 protein in the psiRNA-hH1neo-CK2α transfected group was decreased （0.20±0.09 vs. 0.72±0.16, 0.56±0.11, P 〈0.01）, while the Bax protein level was increased （0.81±0.17 vs. 0.26±0.12, 0.33±0.17, P 〈0.01） and the ratio of Bcl-2 to Bax was decreased （0.25±0.05 vs. 2.76±0.21, 1.70±0.22, P 〈0.01）.Conclusions The siRNA expression plasmid specific to CK2α could suppress CK2α expression and induce the apoptosis of laryngeal carcinoma cells. This is possibly by decreasing the Bcl-2/Bax ratio. CK2α may provide a potential therapeutic target against human laryngeal carcinoma.展开更多
文摘目的探讨静脉性耳鸣患者蛛网膜颗粒内脑疝(brain herniation into arachnoid granulation,BHAG)对于横窦跨狭窄压力梯度(tra ns-stenostic pressure gradient,TPG)升高(≥8m m H g)的诊断效能。方法回顾性分析2010年11月至2023年10月于首都医科大学附属北京友谊医院住院的静脉性耳鸣患者临床及影像学资料,所有患者均行静脉窦非增强M RV(non-contrast enhanced MR venography,NCE-MRV)、全脑MPRAGE及3D-T_(2)WI扫描,并经DSA行静脉窦测压,将患者分为A(TPG<8 mm Hg)、B(TPG≥8 mm Hg)两组。结合全脑MPRAGF及3D-T_(2)WI的标准冠状面图像,评价是否存在BHAG。对A、B两组BHAG发生率是否存在差异进行卡方检验;计算BHAG对于诊断高TPG(≥8 mm Hg)特异度、灵敏度、准确度、阳性预测值及阴性预测值。结果共73例患者符合纳排标准(男性9例,女性64例),年龄37.0(46.5-30.0)岁,其中A组(TPG<8 mm Hg)46例,B组(TPG≥8 mm Hg)27例。BHAG患者共12例(12/73,16.44%),A、B两组BHAG发生率存在统计学差异(χ^(2)值=10.96,P<0.001)。BHAG诊断高TPG(≥8mmHg)的特异度为95.65%、灵敏度为37.04%、准确度为73.97%、阳性预测值为83.33%,阴性预测值为72.13%。结论BHAG在静脉性耳鸣患者中并不罕见,可用于辅助预估有无TPG升高。
文摘Background The relationship between apoptosis and tumors is a major focus in cancer research. RNA interference （RNAi） technology has emerged as a very potent tool to generate cellular knockdown phenotypes of a desired gene. The aim of this study was to explore the effect of siRNA specific to the protein casein kinase 2α （CK2α） on apoptosis of laryngeal carcinoma cells and to explore possible mechanisms.Methods An siRNA expression plasmid specific to CK2α, psiRNA-hH1neo-CK2α, and a non-specific siRNA expression plasmid, psiRNA-hH1neo-cont, were constructed and transfected into Hep-2 cells by a lipofectamine method. The mRNA and protein levels of CK2α in transfected cells were determined by reverse transcription-polymerase chain reaction （RT-PCR） and Western blotting analysis. Apoptosis was measured by Annexin V-fluorescein isothiocyanate （FITC）/ propidium iodide （PI） double-staining methods. The morphological changes to Hep-2 cells were observed under transmission electron microscopy （TEM）. The levels of Bcl-2 and Bax proteins were measured by Western blotting analysis.Results Levels of CK2α mRNA and protein were significantly decreased in the psiRNA-hH1neo-CK2α group compared to the other groups （P 〈0.05）. The apoptotic rate of the psiRNA-hH1neo-CK2α transfected group was significantly higher compared to that in the untransfected group and the siRNA-hH1neo-cont transfected group （25.66%±0.83%, 3.66%±0.43%, and 5.18%±0.22%） （P 〈0.05）. Compared with the untransfected group and the siRNA-hH1neo-cont transfected group, the psiRNA-hH1neo-CK2α transfected group presented with classical ultrastructural features of apoptosis, such as karyopyknosis, chromatic agglutination adjacent to the nuclear membrane, and apoptotic bodies. Compared with the other two groups, the level of Bcl-2 protein in the psiRNA-hH1neo-CK2α transfected group was decreased （0.20±0.09 vs. 0.72±0.16, 0.56±0.11, P 〈0.01）, while the Bax protein level was increased （0.81±0.17 vs. 0.26±0.12, 0.33±0.17, P 〈0.01） and the ratio of Bcl-2 to Bax was decreased （0.25±0.05 vs. 2.76±0.21, 1.70±0.22, P 〈0.01）.Conclusions The siRNA expression plasmid specific to CK2α could suppress CK2α expression and induce the apoptosis of laryngeal carcinoma cells. This is possibly by decreasing the Bcl-2/Bax ratio. CK2α may provide a potential therapeutic target against human laryngeal carcinoma.
