药物球囊在股腘动脉支架内再狭窄中运用的中期随访结果

目的评价紫杉醇药物涂层球囊(drug-coated balloon,DCB)在股腘动脉支架内再狭窄(in-stent restenosis,ISR)血管腔内治疗中的临床效果。方法对2016年12月至2020年7月接受DCB治疗的股腘动脉ISR患者进行回顾性分析。评价腔内操作的围手术期并发症发生率,术后12、18个月靶血管一期通畅率、一期辅助通畅率、二期通畅率、免于临床症状驱动的靶病变血运重建率(free from clinically-driven target lesion revascularization,F-TLR)、大截肢率以及全因死亡率。结果共52例ISR患者(56条下肢)接受了DCB治疗。根据Rutherford分级:2级1例(1.7%),3级9例(23.2%),4级23例(41.1%),5级15例(26.8%),6级4例(7.1%)。Tosaka Ⅱ级ISR 46条下肢(82.1%),Ⅲ级ISR 10条下肢(17.9%)。平均ISR长度(240±122)mm。一期补救性支架植入率25%。中位随访时间18个月。全因死亡率11.8%、大截肢率为5.9%、一期通畅率53.4%、一期辅助通畅率67.1%、二期通畅率93.2%、F-TLR为77.2%。结论 DCB是一种安全有效的股腘动脉ISR血管腔内治疗手段。

DGP1,a drought-induced guard cell-specific promoter and its function analysis in tobacco plants

The genetic regulation of stomatal movement mainly depends on an efficient control system of gene expression,and guard cell-specific promoter is becoming the best choice.Here we combined the dehydration responsive element(DRE)with guard cell specific element(GCSE)to construct a novel promoter,DGP1.Histochemical assays in transgenic tobacco carryingβ-glucuronidase(gus)gene fused to DGP1 demonstrated that GUS activity was found to be highly inducible by drought treatment and specifically restricted to guard cells.No GUS activity was detected in roots,stems or flowers after treatment.Further quantitative analysis showed that GUS activity in the epidermal strips was apparently induced by dehydration and dramatically increased with the elongation of treatment.The GUS activity after 8 h treatment was 179 times that of those without treatment.Although GUS activity in roots,stems or mesophyll increased after treatment,no great changes were observed.These results suggested that DGP1 could drive target gene expressed in guard cells when plant is subjected to drought stress.And this gets us prepared to control opening and closing of stomata through plant gene engineering.