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藏猪和大约克猪CKM基因多态性及表达差异分析 被引量:2
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作者 巩兴龙 段梦琪 +5 位作者 张健 李雨晴 张芳芳 赵雪 强巴央宗 商鹏 《西南农业学报》 CSCD 北大核心 2021年第1期197-201,共5页
【目的】本研究旨在探究猪CKM基因的多态性及其mRNA表达水平,通过对CKM基因起始密码子上游3 kb区域进行多态性检测,发现了单核苷酸多态性位点C-1185T和C-1324T,且该位点符合哈迪-温伯格定律(P<0.05)。【方法】利用实时荧光定量PCR技... 【目的】本研究旨在探究猪CKM基因的多态性及其mRNA表达水平,通过对CKM基因起始密码子上游3 kb区域进行多态性检测,发现了单核苷酸多态性位点C-1185T和C-1324T,且该位点符合哈迪-温伯格定律(P<0.05)。【方法】利用实时荧光定量PCR技术对CKM基因在藏猪和大约克猪不同组织的mRNA表达量进行检测。【结果】发现在同一品种猪种,CKM基因的mRNA表达水平从高至低依次为背最长肌>背脂>肝脏组织,不同猪种内CKM基因在背最长肌、背脂和肝脏组织中存在差异表达,在背最长肌中,藏猪(TP)极显著高于大约克猪(YY)(P<0.01),在背脂中,大约克猪(YY)显著高于藏猪(TP)(P<0.05),在肝脏组织中,藏猪(TP)极显著高于大约克猪(YY)(P<0.01)。【结论】推测CKM基因与骨骼肌的生长发育呈负相关。本研究为下一步深入开展CKM基因功能研究奠定基础。 展开更多
关键词 CKM基因 多态性 基因表达
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Impact failure models and application condition of trees in debris-flow hazard mitigation 被引量:1
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作者 JIN Ke CHEN Jian-gang +3 位作者 CHEN Xiao-qing ZHAO Wan-yu SI Guang-wu gong xing-long 《Journal of Mountain Science》 SCIE CSCD 2021年第7期1874-1885,共12页
Forestry has played an important role in hazard mitigation associated with debris flows.Most forest mitigation measures refer to the experience of soil and water conservation,which disregard the destructive effect of ... Forestry has played an important role in hazard mitigation associated with debris flows.Most forest mitigation measures refer to the experience of soil and water conservation,which disregard the destructive effect of debris flows,causing potentially serious consequences.Determination of the effect of a forest on reducing debris-flow velocity and even stopping debris flows requires distinguishing between when the debris flow will destroy the forest and when the trees will withstand the debris-flow impact force.In this paper,we summarized two impact failure models of a single tree: stem breakage and overturning.The influences of different tree sizes characteristics(stem base diameter,tree weight,and root failure radius) and debris-flow characteristics(density,velocity,flow depth,and boulder diameter) on tree failure were analyzed.The observations obtained from the model adopted in this study show that trees are more prone to stem breakage than overturning.With an increase in tree size,the ability to resist stem breakage and overturning increases.Debris-flow density influences the critical failure conditions of trees substantially less than the debrisflow velocity,depth,and boulder diameter.The application conditions of forests in debris-flow hazard mitigation were proposed based on the analysis of the model results.The proposed models were applied in the Xiajijiehaizi Gully as a case study,and the results explain the destruction of trees in the forest dispersing zone.This work provides references for implementing forest measures for debris-flow hazard mitigation. 展开更多
关键词 Trees Impact failure model Forest application condition Debris flow mitigation
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基于输电线路属地化联责管理的探索与实践 被引量:1
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作者 宫兴龙 《电力系统装备》 2019年第3期198-199,共2页
近年来,随着我国的经济不断发展,加速了我国工业化的建设,城市规模也逐渐扩大,这导致了我国的建筑用地开始紧张起来,在农业与林业不断发展的情况下,部分输电线路遭到破坏,特别是一些违章施工和房建情况严重,在输电保护区内未经审批就进... 近年来,随着我国的经济不断发展,加速了我国工业化的建设,城市规模也逐渐扩大,这导致了我国的建筑用地开始紧张起来,在农业与林业不断发展的情况下,部分输电线路遭到破坏,特别是一些违章施工和房建情况严重,在输电保护区内未经审批就进行违章建筑,森林防火问题严重,导致线路经常跳闸,加大了对我国电力系统运行的威胁。为了保障电力系统的运行安全,我国电力部门总结经验教训,开展了输电线路属地化联责管理的探索,改善了输电网的运行环境,有效的保证了我国输电线路的运行安全。 展开更多
关键词 输电线路 属地化 联责管理
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