OBJECTIVE: To investigate the involvement of growth arrest specific 5(GAS5), a long non-coding RNA, in the anti-hepatic fibrosis process induced by Dahuang Zhechong pill(DHZCP) in rats.METHODS: Thirty adult rats were ...OBJECTIVE: To investigate the involvement of growth arrest specific 5(GAS5), a long non-coding RNA, in the anti-hepatic fibrosis process induced by Dahuang Zhechong pill(DHZCP) in rats.METHODS: Thirty adult rats were divided into three groups, including a control group, a CCl4-induced fibrosis group and a DHZCP-treated fibrosis group. Hematoxylin-eosin and Masson staining were used for histopathological study. Serum enzymes, cytokines and cell proliferation were assayed using commercially available kits. A GAS5 lenti-virus vector was constructed to further investigate the role of GAS5 in the anti-hepatic fibrosis effect of DHZCP in rats.RESULTS: Our results revealed that the proliferation of hepatic stellate cells cultured in serum derived from rats treated with DHZCP was significantly decreased, compared with cells treated with serum from the untreated rats. DHZCP alleviated the CCl4-induced hepatic fibrosis. Additionally, DHZCP can restore the expression of GAS5,which was significantly decreased in the CCl4-induced group, and markedly suppress the expression of p-p38 and p-Erk induced by CCl4, but not p-Jnk. Cell proliferation was significantly arrested when cells overexpressed GAS5. Thus,DHZCP can inhibit the expression of p-p38 and p-Erk, while GAS5 can only inhibit the expression of p-Erk.CONCLUSIONS: DHZCP can alleviate hepatic fibrosis by increasing the expression of GAS5 to suppress p-Erk and regulating other factors to inhibit p-p38.展开更多
Water-jet propulsion is a widely applied ship propulsion technology.Its steering control system has an important impact on manoeuvre performance.In this paper,transfer function model is firstly established on the basi...Water-jet propulsion is a widely applied ship propulsion technology.Its steering control system has an important impact on manoeuvre performance.In this paper,transfer function model is firstly established on the basis of mechanism analysis of water-jet steering system.Then,by considering the variability of model parameters and input constraints in practical operation,a model predictive controller is designed for steering system control.Subsequently,model based disturbance observer is employed in an attempt to reject environmental disturbances.The performance of the proposed model predictive control (MPC) scheme for a particular steering system is compared with that of conventional proportional integral derivative (PID) control strategy.Simulation results demonstrate that the proposed model predictive controller outperforms conventional PID controller,particularly in robustness,response delay and tracking accuracy.展开更多
基金Supported by National Natural Science Foundation of China:Research on Molecular Mechanism of Anti Liver Fibrosis of Dahuang Zhecong pill Targeting on NADPH Oxidase Signal Network(No.81473473)Science Technology and Innovation Commission of Shenzhen Municipality:the Role of Lnc RNA GAS5 on the Anti-hepatic Fibrosis of Dahuang Zhechong pill(No.JCYJ201604281732-52471)
文摘OBJECTIVE: To investigate the involvement of growth arrest specific 5(GAS5), a long non-coding RNA, in the anti-hepatic fibrosis process induced by Dahuang Zhechong pill(DHZCP) in rats.METHODS: Thirty adult rats were divided into three groups, including a control group, a CCl4-induced fibrosis group and a DHZCP-treated fibrosis group. Hematoxylin-eosin and Masson staining were used for histopathological study. Serum enzymes, cytokines and cell proliferation were assayed using commercially available kits. A GAS5 lenti-virus vector was constructed to further investigate the role of GAS5 in the anti-hepatic fibrosis effect of DHZCP in rats.RESULTS: Our results revealed that the proliferation of hepatic stellate cells cultured in serum derived from rats treated with DHZCP was significantly decreased, compared with cells treated with serum from the untreated rats. DHZCP alleviated the CCl4-induced hepatic fibrosis. Additionally, DHZCP can restore the expression of GAS5,which was significantly decreased in the CCl4-induced group, and markedly suppress the expression of p-p38 and p-Erk induced by CCl4, but not p-Jnk. Cell proliferation was significantly arrested when cells overexpressed GAS5. Thus,DHZCP can inhibit the expression of p-p38 and p-Erk, while GAS5 can only inhibit the expression of p-Erk.CONCLUSIONS: DHZCP can alleviate hepatic fibrosis by increasing the expression of GAS5 to suppress p-Erk and regulating other factors to inhibit p-p38.
基金the Open Project of the Key Laboratory of Science and Technology on Waterjet Propulsion(No.614222303051117)the Joint Fund of CSSC(No.6141B03020301)。
文摘Water-jet propulsion is a widely applied ship propulsion technology.Its steering control system has an important impact on manoeuvre performance.In this paper,transfer function model is firstly established on the basis of mechanism analysis of water-jet steering system.Then,by considering the variability of model parameters and input constraints in practical operation,a model predictive controller is designed for steering system control.Subsequently,model based disturbance observer is employed in an attempt to reject environmental disturbances.The performance of the proposed model predictive control (MPC) scheme for a particular steering system is compared with that of conventional proportional integral derivative (PID) control strategy.Simulation results demonstrate that the proposed model predictive controller outperforms conventional PID controller,particularly in robustness,response delay and tracking accuracy.
