A potent D-Trp^6-GnRH iodinated by the lactoperoxidase glucose oxidase method was used as a ligand to bind specifically to the rat ovarian membrane, The ovariu GnRH receptor has a binding affinity with the agonist sim...A potent D-Trp^6-GnRH iodinated by the lactoperoxidase glucose oxidase method was used as a ligand to bind specifically to the rat ovarian membrane, The ovariu GnRH receptor has a binding affinity with the agonist similar to that of the pituiary receptor,展开更多
Suspensions of luteal cells were obtained from human corpora lutea in mid-luteal phase (day 19-23) by enzymatic dispersion. Enriched fractions of large and small luteal cells were prepared by centrifugation on a stepp...Suspensions of luteal cells were obtained from human corpora lutea in mid-luteal phase (day 19-23) by enzymatic dispersion. Enriched fractions of large and small luteal cells were prepared by centrifugation on a stepped gradient of Pcrcoll.展开更多
Theeffects of gonadotrophin--relensing hormone (GnRH) onthe bindingof^125I-labelled GnRH agonist to human placental membranes were studied. The GnRH binding sites of human plaoenta had a high specificity but low affin...Theeffects of gonadotrophin--relensing hormone (GnRH) onthe bindingof^125I-labelled GnRH agonist to human placental membranes were studied. The GnRH binding sites of human plaoenta had a high specificity but low affinity. The natural GnRH had a展开更多
文摘A potent D-Trp^6-GnRH iodinated by the lactoperoxidase glucose oxidase method was used as a ligand to bind specifically to the rat ovarian membrane, The ovariu GnRH receptor has a binding affinity with the agonist similar to that of the pituiary receptor,
文摘Suspensions of luteal cells were obtained from human corpora lutea in mid-luteal phase (day 19-23) by enzymatic dispersion. Enriched fractions of large and small luteal cells were prepared by centrifugation on a stepped gradient of Pcrcoll.
文摘Theeffects of gonadotrophin--relensing hormone (GnRH) onthe bindingof^125I-labelled GnRH agonist to human placental membranes were studied. The GnRH binding sites of human plaoenta had a high specificity but low affinity. The natural GnRH had a
