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DNA-SIP鉴定甘蔗//大豆间作土壤^(15)N-DNA富集位置的氮循环功能基因qPCR方法 被引量:2
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作者 苟永刚 余玲玲 +1 位作者 许霞 王建武 《农业环境科学学报》 CAS CSCD 北大核心 2019年第1期140-147,共8页
为筛选有效鉴定甘蔗//大豆间作系统的稳定性同位素核酸探针技术(DNA-SIP)中超高速离心后15N-DNA富集位置的指示功能基因,利用实时荧光定量PCR技术(qPCR),检测6个氮素循环功能基因在不同浮力密度离心液DNA中的相对丰度分布,通过对氮素循... 为筛选有效鉴定甘蔗//大豆间作系统的稳定性同位素核酸探针技术(DNA-SIP)中超高速离心后15N-DNA富集位置的指示功能基因,利用实时荧光定量PCR技术(qPCR),检测6个氮素循环功能基因在不同浮力密度离心液DNA中的相对丰度分布,通过对氮素循环功能基因相对丰度作图分析,nifH和amoA基因在甘蔗//大豆间作和大豆单作种植模式中15N标记组与对照组基因丰度峰发生偏移,chiA基因丰度峰仅在大豆单作种植模式下存在偏移,而nirS、nirK、nosZ等3个基因的丰度峰值在两种种植模式下均不发生偏移。结果表明nifH和amoA基因可作为指示基因,能够有效鉴定甘蔗//大豆间作系统中DNA-SIP技术15N-DNA位置。 展开更多
关键词 甘蔗//大豆间作 稳定性同位素核酸探针 荧光定量PCR 功能基因
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Soil mineral nitrogen and yield-scaled soil N2O emissions lowered by reducing nitrogen application and intercropping with soybean for sweet maize production in southern China 被引量:4
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作者 TANG Yi-ling YU Ling-ling +4 位作者 GUAN Ao-mei ZHOU Xian-yu WANG Zhi-guo gou yong-gang WANG Jian-wu 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2017年第11期2586-2596,共11页
The increasing demand for fresh sweet maize (Zea mays L. saccharata) in southern China has prioritized the need to find solutions to the environmental pollution caused by its continuous production and high inputs of... The increasing demand for fresh sweet maize (Zea mays L. saccharata) in southern China has prioritized the need to find solutions to the environmental pollution caused by its continuous production and high inputs of chemical nitrogen fertilizers. A promising method for improving crop production and environmental conditions is to intercrop sweet maize with legumes. Here, a three-year field experiment was conducted to assess the influence of four different cropping systems (sole sweet maize (SS), sole soybean (SB), two rows sweet maize-three rows soybean (S2B3) intercropping, and two rows sweet maize-four rows soybean (S2B4) intercropping), together with two rates of N fertilizer application (300 and 360 kg N ha-1) on grain yield, residual soil mineral N, and soil N2O emissions in southern China. Results showed that in most case, inter- cropping achieved yield advantages (total land equivalent ratio (TLER=0.87-1.25) was above one). Moreover, intercropping resulted in 39.8% less soil mineral N than SS at the time of crop harvest, averaged over six seasons (spring and autumn in each of the three years of the field experiment). Generally, intercropping and reduced-N application (300 kg N ha-1) produced lower cumulative soil N20 and yield-scaled soil N20 emissions than SS and conventionaI-N application (360 kg N ha-l), respectively. $2B4 intercropping with reduced-N rate (300 kg N ha-~) showed the lowest cumulative soil N20 (mean value=0.61 kg ha-1) and yield-scaled soil N20 (mean value=0.04 kg t-1) emissions. Overall, intercropping with reduced-N rate maintained sweet maize production, while also reducing environmental impacts. The system of S2B4 intercropping with reduced-N rate may be the most sustainable and environmentally friendly cropping system. 展开更多
关键词 sweet maize-soybean intercrop cropping system N fertilizer rate grain yield soil mineral N soil NzO emissions
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