基于响应面法优化紫花地丁多酚与黄酮的提取工艺

该文研究超声辅助提取紫花地丁中多酚、黄酮的最佳工艺条件。基于微波超声技术,通过乙醇提取方法,以多酚、黄酮提取量为主要指标,运用响应面技术得出最佳提取工艺。结果表明,超声时间、功率和料液比是影响黄酮、多酚提取最重要的因素,多酚最优提取工艺为超声时间9 min、超声功率210 W、料液比1∶14(g/mL)。黄酮最佳提取工艺为超声时间11 min、超声功率215 W、料液比1∶13(g/mL)。此工艺下提取紫花地丁的多酚提取量为29.04 mg/g,黄酮提取量为28.02 mg/g。

枇杷叶HPLC特征图谱研究及主要成分含量测定

目的:建立枇杷叶的HPLC特征图谱,并测定其中主要成分金丝桃苷、槲皮苷的含量。方法:色谱柱为CAPCELL PAK MG C18(250 mm×4.6 mm,5μm);流动相为乙腈-0.1%磷酸水溶液,梯度洗脱;流速1.0 mL·min-1;检测波长355 nm;柱温30℃;进样量20μL。建立枇杷叶药材的特征图谱,并采用《中药色谱指纹图谱相似度评价系统(2012年版)》进行分析。结果:从枇杷叶药材特征图谱中共标示出10个共有峰,各共有峰相对保留时间RSD均小于2.0%。主要成分金丝桃苷和槲皮苷分别在0.1026~0.8550、0.0858~0.7150μg线性关系良好,平均加样回收率分别为99.76%、95.56%,RSD分别为4.88%、4.84%。结论:所建立的方法操作简便、重复性好,可为有效控制枇杷叶药材的内在质量标准提供参考。

Current Status and Research Trends of Panax Between 1900—2019:A Bibliometric Analysis

Objective:To investigate the current status and further dtevelopment of Panax genus and6 important individual species including P.notoginseng,P.quinquefolium,P.vietnamensis,P.japonicus,P.stipuleanatus and P.zingiberensis.Methods:The bibliometric analysis was based on the Web of Science core database platform from Thomson Reuters.Totally,7,574 records of scientific research of Panax species published from 1900—2019 were analyzed.The statistical and visualization analysis was performed by CiteSpace and HistCite software.Results:The academic research of Panax species increase promptly.Plant science is the main research field while research and experimental medicine and agricultural engineering will be the further development tendency.Particularly,the discrimination research of P.notoginseng will be the research tendency among Panax species,especially diversity research.In addition,P.vietnamensis deserves more attention in the genus Panax.Conclusion:This research provides a reference for further research of the genus and individual species.