The retinoblastoma (Rb) suppressor associated protein 46 ( RbAp46 ) also named retinoblastoma binding protein 7 (RBBP7) was first identified as a protein in HeLa cells that binds to an Rb affinity column. RbAp46...The retinoblastoma (Rb) suppressor associated protein 46 ( RbAp46 ) also named retinoblastoma binding protein 7 (RBBP7) was first identified as a protein in HeLa cells that binds to an Rb affinity column. RbAp46 has been shown to be a core component of the mSin3 histone deacetylase (HDAC) complex and NuRD ( a multi-subunit complex containing chromosome-remodeling activity). 2 RbAp46 is also known as the histone acetyltransferase (HAT) type B subunit展开更多
文摘目的:探讨miR-155在慢性淋巴细胞白血病(chronic lymphocytic leukemia,CLL)患者外周血B细胞中的表达水平及其与患者的临床生物学特征之间的相关性。方法:收集73例初诊CLL患者的外周血单个核细胞及60例健康对照人群外周血CD19+B细胞,提取细胞总RNA,并采用qRT-PCR方法测定细胞内miR-155表达。系统性分析CLL患者临床特征,分析miR-155表达与CLL患者的临床表现,如年龄、性别、Binet分期、细胞遗传学特征及分子生物学特征的相关性,探讨miR-155与患者首次治疗时间(time to treatment,TTT)和总生存期(overall survival,OS)之间的关系。结果:MiR-155在CLL患者中的表达较正常人群明显增高。比较不同分子生物学特征的CLL患者中miR-155表达水平结果提示,免疫球蛋白重链可变区(immunoglobulin heavy chian variable region,IGHV)存在突变的CLL患者miR-155的表达较IGHV无突变的患者低(P<0.05),同时在IGHV4-39家族中miR-155的表达明显偏低(P<0.05)。荧光原位杂交(fluorescence in situ hybridization,FISH)检测结果显示,p53突变/缺失阳性及ATM基因缺失阳性的患者miR-155的表达增高(P<0.05)。OS及TTT在miR-155高表达组与低表达组之间无差异。结论:MiR-155在CLL患者中表达升高,同时其高表达与其临床不良预后相关。这提示,Mi R-155在CLL发生与发展中具有重要作用。
文摘The retinoblastoma (Rb) suppressor associated protein 46 ( RbAp46 ) also named retinoblastoma binding protein 7 (RBBP7) was first identified as a protein in HeLa cells that binds to an Rb affinity column. RbAp46 has been shown to be a core component of the mSin3 histone deacetylase (HDAC) complex and NuRD ( a multi-subunit complex containing chromosome-remodeling activity). 2 RbAp46 is also known as the histone acetyltransferase (HAT) type B subunit