This paper studies the extraction and identification of ginsenoside Re from ginseng fruits and investigates the effect and mechanism of ginsenoside Re of protecting acute renal ischemia-reperfusion injury in rats.Havi...This paper studies the extraction and identification of ginsenoside Re from ginseng fruits and investigates the effect and mechanism of ginsenoside Re of protecting acute renal ischemia-reperfusion injury in rats.Having been smashed,the ginseng fruits were ultrasonically extracted twice with 95% ethanol for 30min each.Having been concentrated,the solution was dissolved with distilled water and separated by two-column chromatography,of which one was packed with macroporous resin D4020,and the other was packed with macroporous resin D941.The raw product was dissolved with methanol and was purified by elution on a Si gel column,finally ginsenoside Re was obtained.The structure of the ginsenoside Re was analyzed by the thin-layer chromatography and NMR methods,and HPLC was carried for the content determination.The model of acute renal ischemia-reperfusion injury in rats was established after ischemia for 1 h and reperfusion for 1 h or 24 h,serum SOD(superoxide dismutase),MDA(malondialdehyde) and plasma TXB2(thromboxane B2) and 6-keto-PGF1α were detected.The results show that it has accurately,fast,convenient merits and so on.Ginsenoside Re has a protective effect on acute renal ischemia-reperfusion injury in rats,the mechanism may be related to improving the imbalance of thromboxane A2(TXA2)/prostacyclin(PGI2) and inhibiting lipid peroxidation reaction.展开更多
The authors chose specimens from nine normal prostate tissues(NP group), 15 benign prostate hyperplasia(BPH) prostates(BPH group), and 35 BPH prostates that had been treated'with ^90Sr/^90Y Prostatic Hyperplasi...The authors chose specimens from nine normal prostate tissues(NP group), 15 benign prostate hyperplasia(BPH) prostates(BPH group), and 35 BPH prostates that had been treated'with ^90Sr/^90Y Prostatic Hyperplasia Applicator(exposure group). The expressions of bcl-2 and bax in stroma and epithelia of prostate tissues were demonstrated by means of immunohistochemical staining, and the staining positive rate was semiquantatively determined, so as to observe the expression of bcl-2 and bax genes in the prostate tissues of normal individuals and BPH patients, before and after fl radiation, and to evaluate the influence of fl radiation on bcl-2 and bax expressions. The expressions of gene bcl-2 in the prostate epithelia of NP and BPH are significantly higher than those in the prostate stroma(P〈0.01). However, the expressions of bcl-2 in the prostate epithelia and stroma of the BPH group are obviously higher than those in the NP group(P〈0.01). The expression of gene bax in the prostate epithelia of the NP group is higher than that in the BPH group(P〈0.05). However, bcl-2 expressions in the prostate epithelia and stroma of the BPH group are significantly higher than the bax expressions(P〈0.01). Compared with those of the NP group, the expressions of bcl-2 in the prostate epithelia and stroma of the exposure group decrease remarkably, even as the expressions of the bax notably increase(P〈0.01). Thus, the administration of β radiation can remarkably affect bcl-2 and bax gene expressions, to regulate cell apoptosis, in the prostate tissues of BPH.展开更多
基金Supported by the National Natural Science Foundation of China(No.30701128)the Fund of Jilin Provincial Science and Technology Department,China(No.200705377)
文摘This paper studies the extraction and identification of ginsenoside Re from ginseng fruits and investigates the effect and mechanism of ginsenoside Re of protecting acute renal ischemia-reperfusion injury in rats.Having been smashed,the ginseng fruits were ultrasonically extracted twice with 95% ethanol for 30min each.Having been concentrated,the solution was dissolved with distilled water and separated by two-column chromatography,of which one was packed with macroporous resin D4020,and the other was packed with macroporous resin D941.The raw product was dissolved with methanol and was purified by elution on a Si gel column,finally ginsenoside Re was obtained.The structure of the ginsenoside Re was analyzed by the thin-layer chromatography and NMR methods,and HPLC was carried for the content determination.The model of acute renal ischemia-reperfusion injury in rats was established after ischemia for 1 h and reperfusion for 1 h or 24 h,serum SOD(superoxide dismutase),MDA(malondialdehyde) and plasma TXB2(thromboxane B2) and 6-keto-PGF1α were detected.The results show that it has accurately,fast,convenient merits and so on.Ginsenoside Re has a protective effect on acute renal ischemia-reperfusion injury in rats,the mechanism may be related to improving the imbalance of thromboxane A2(TXA2)/prostacyclin(PGI2) and inhibiting lipid peroxidation reaction.
基金the Science Research Foundation of Jilin University(No.450011022007)WU Jie-ping Medical Foundation (No.320.6750.06117)
文摘The authors chose specimens from nine normal prostate tissues(NP group), 15 benign prostate hyperplasia(BPH) prostates(BPH group), and 35 BPH prostates that had been treated'with ^90Sr/^90Y Prostatic Hyperplasia Applicator(exposure group). The expressions of bcl-2 and bax in stroma and epithelia of prostate tissues were demonstrated by means of immunohistochemical staining, and the staining positive rate was semiquantatively determined, so as to observe the expression of bcl-2 and bax genes in the prostate tissues of normal individuals and BPH patients, before and after fl radiation, and to evaluate the influence of fl radiation on bcl-2 and bax expressions. The expressions of gene bcl-2 in the prostate epithelia of NP and BPH are significantly higher than those in the prostate stroma(P〈0.01). However, the expressions of bcl-2 in the prostate epithelia and stroma of the BPH group are obviously higher than those in the NP group(P〈0.01). The expression of gene bax in the prostate epithelia of the NP group is higher than that in the BPH group(P〈0.05). However, bcl-2 expressions in the prostate epithelia and stroma of the BPH group are significantly higher than the bax expressions(P〈0.01). Compared with those of the NP group, the expressions of bcl-2 in the prostate epithelia and stroma of the exposure group decrease remarkably, even as the expressions of the bax notably increase(P〈0.01). Thus, the administration of β radiation can remarkably affect bcl-2 and bax gene expressions, to regulate cell apoptosis, in the prostate tissues of BPH.