巢式关联作图(Nested Association Mapping,NAM)群体在作物学遗传与育种研究中具有广泛的应用。本研究在前期大豆种质资源评价基础上,利用35份不同地区来源的代表性种质与中豆41(公共母本)杂交,构建了一套大豆NAM群体。PCA和聚类分析发...巢式关联作图(Nested Association Mapping,NAM)群体在作物学遗传与育种研究中具有广泛的应用。本研究在前期大豆种质资源评价基础上,利用35份不同地区来源的代表性种质与中豆41(公共母本)杂交,构建了一套大豆NAM群体。PCA和聚类分析发现,不同亲本组合的RIL群体基本聚在一起,显示出清晰的遗传结构。利用该NAM群体亲本间花色和种皮色具有显著差异的RIL群体进行全基因组关联分析,定位到1个主要位点qFC13-1与花色显著关联,该位点与W1位点重合;定位到12个位点与种皮色显著相关,其中9个位点为3种以上方法共定位,3个位点为2种方法共定位,包括4个已知位点和8个新位点。研究结果表明,构建的NAM群体适于进行大豆相关性状遗传分析,为大豆复杂性状的遗传解析和育种实践提供了良好的基础材料。展开更多
室内生物测定是农作物除草剂耐受性鉴定的常用方法,已广泛应用于大豆、棉花等作物对草甘膦、氯嘧磺隆等除草剂的耐受性研究,但莠去津室内生测方法及大豆对莠去津耐受性相关研究鲜见报道。本研究以莠去津对大豆萌发期各单项指标为评价指...室内生物测定是农作物除草剂耐受性鉴定的常用方法,已广泛应用于大豆、棉花等作物对草甘膦、氯嘧磺隆等除草剂的耐受性研究,但莠去津室内生测方法及大豆对莠去津耐受性相关研究鲜见报道。本研究以莠去津对大豆萌发期各单项指标为评价指标,分析莠去津胁迫条件下大豆发芽势、发芽率、发芽指数、活力指数、根长等性状的变化,建立大豆莠去津耐受性室内生测方法,确定3.5 mL L^(-1)是大豆萌发期莠去津耐受性筛选的适宜浓度;对159份大豆种质进行萌发期莠去津耐受性鉴定表明,各指标相对值的变异系数为发芽相对莠去津胁迫率>相对芽长>相对发芽指数>相对活力指数>相对发芽势>相对发芽率;根据T值的聚类分析,将供试种质分为4个莠去津耐性级别,共鉴定出高耐种质9份、中耐种质50份、低耐种质70份和敏感种质30份;黄豆、花色豆、垦丰13、南农99-6等9个种质为莠去津耐受性综合能力优异的种质资源。采用多元逐步回归分析法,建立大豆萌发期耐莠去津评价模型Y=–0.155+0.004X_(2)+0.001X_(3)+0.001X_(4)+0.002X_(5)(P=0.000)。本研究为培育耐莠去津育种的亲本选配、后代选择及大豆耐莠去津相关基因挖掘提供了理论依据、材料和技术支撑。展开更多
With the development of sequencing technology, insertions-deletions(InDels) have been increasingly reported to be involved in the genetic determination of agronomical traits. However, most studies have focused on the ...With the development of sequencing technology, insertions-deletions(InDels) have been increasingly reported to be involved in the genetic determination of agronomical traits. However, most studies have focused on the identification and application of short-InDels(1–15 bp) for genetic analysis. The objective of this study was to deeply deploy long-InDels(>15 bp) for the genetic analysis of important agronomic traits in soybean. A total of 13 573 polymorphic long-InDels were identified between parents Zhongpin 03-5373(ZP) and Zhonghuang 13(ZH), which were unevenly distributed on 20 chromosomes of soybean, varying from 321 in Chr11 to 1 246 in Chr18. Consistent with the distribution pattern of annotated genes, the average density of long-InDels in arm regions was significantly higher than that in pericentromeric regions at the P=0.01 level. A total of 2 704(19.9% of total) long-InDels were located in genic regions, including 319 large-effect long-InDels, which resulted in truncated or elongated protein sequences. A previously identified QTL(qP H16) underlying plant height was further analyzed, and it was found that 26 out of 35(74.3%) long-InDel markers located in the qPH16 region showed clear polymorphisms between parents ZP and ZH. Seven markers, including three long-InDels and four previously reported SNP markers, were used to genotype 242 recombinant inbred lines derived from ZP×ZH. As a result, the qPH16 locus was narrowed from a 960-kb region to a 477.55-kb region, containing 65 annotated genes. Therefore, these long-InDels are a complementary genetic resource of SNPs and short-InDels for plant height and can facilitate genetic studies and molecular assisted selection breeding in soybean.展开更多
In order to screen the aroma characteristics of soybean,a new method was established which can quickly quantify the content of 2-acetyl-1pyrroline(2-AP),an important compound related to soybean aroma,using gas chromat...In order to screen the aroma characteristics of soybean,a new method was established which can quickly quantify the content of 2-acetyl-1pyrroline(2-AP),an important compound related to soybean aroma,using gas chromatography-mass spectrometry(GC-MS).Based on peak profile,total peak area and retention time as test indexes,an accurate identification method for fragrant soybeans was established.The optimum parameters of the protocol consisted of column temperature70℃,sample injector temperature 180℃,optimum extraction alcohol content 1 m L,Na Cl content 0.1 g,ultrasonication time10 min,and extraction time 1 h,which were established by using the orthogonal test of single factors and three factors with four levels(L_9(3)^4).2-AP content of leaves had significant correlations with seeds,which were easier to measure.The protocol was simple and easy to carry out,consumed only small amounts of reagents,and provided accurate and reliable results with good reproducibility.A total of 101 soybean genotypes from different geographical sources were analyzed using this protocol.The results showed that the average content of 2-AP was 0.29 mg L^(–1),ranging from 0.094 to 1.816 mg L^(–1),and the genetic diversity index was 0.54.Among all genotypes-tested,they were classified into three grades,including seven elite genotypes identified as"grade one fragrant soybeans",which were Zhonglong 608,Heinong 88,Ha13-2958,Hongmiandou,Heinong 82,Huangmaodou,and Jiyu 21.These results provide both an identification technique and several elite aroma genotypes for gene discovery and good quality breeding in soybean.展开更多
文摘巢式关联作图(Nested Association Mapping,NAM)群体在作物学遗传与育种研究中具有广泛的应用。本研究在前期大豆种质资源评价基础上,利用35份不同地区来源的代表性种质与中豆41(公共母本)杂交,构建了一套大豆NAM群体。PCA和聚类分析发现,不同亲本组合的RIL群体基本聚在一起,显示出清晰的遗传结构。利用该NAM群体亲本间花色和种皮色具有显著差异的RIL群体进行全基因组关联分析,定位到1个主要位点qFC13-1与花色显著关联,该位点与W1位点重合;定位到12个位点与种皮色显著相关,其中9个位点为3种以上方法共定位,3个位点为2种方法共定位,包括4个已知位点和8个新位点。研究结果表明,构建的NAM群体适于进行大豆相关性状遗传分析,为大豆复杂性状的遗传解析和育种实践提供了良好的基础材料。
文摘室内生物测定是农作物除草剂耐受性鉴定的常用方法,已广泛应用于大豆、棉花等作物对草甘膦、氯嘧磺隆等除草剂的耐受性研究,但莠去津室内生测方法及大豆对莠去津耐受性相关研究鲜见报道。本研究以莠去津对大豆萌发期各单项指标为评价指标,分析莠去津胁迫条件下大豆发芽势、发芽率、发芽指数、活力指数、根长等性状的变化,建立大豆莠去津耐受性室内生测方法,确定3.5 mL L^(-1)是大豆萌发期莠去津耐受性筛选的适宜浓度;对159份大豆种质进行萌发期莠去津耐受性鉴定表明,各指标相对值的变异系数为发芽相对莠去津胁迫率>相对芽长>相对发芽指数>相对活力指数>相对发芽势>相对发芽率;根据T值的聚类分析,将供试种质分为4个莠去津耐性级别,共鉴定出高耐种质9份、中耐种质50份、低耐种质70份和敏感种质30份;黄豆、花色豆、垦丰13、南农99-6等9个种质为莠去津耐受性综合能力优异的种质资源。采用多元逐步回归分析法,建立大豆萌发期耐莠去津评价模型Y=–0.155+0.004X_(2)+0.001X_(3)+0.001X_(4)+0.002X_(5)(P=0.000)。本研究为培育耐莠去津育种的亲本选配、后代选择及大豆耐莠去津相关基因挖掘提供了理论依据、材料和技术支撑。
基金supported by the National Key R&D Program of China(2016YFD0100201 and 2020YFE0202300)the Agricultural Science and Technology Innovation Program(ASTIP)of the Chinese Academy of Agricultural Sciences。
文摘With the development of sequencing technology, insertions-deletions(InDels) have been increasingly reported to be involved in the genetic determination of agronomical traits. However, most studies have focused on the identification and application of short-InDels(1–15 bp) for genetic analysis. The objective of this study was to deeply deploy long-InDels(>15 bp) for the genetic analysis of important agronomic traits in soybean. A total of 13 573 polymorphic long-InDels were identified between parents Zhongpin 03-5373(ZP) and Zhonghuang 13(ZH), which were unevenly distributed on 20 chromosomes of soybean, varying from 321 in Chr11 to 1 246 in Chr18. Consistent with the distribution pattern of annotated genes, the average density of long-InDels in arm regions was significantly higher than that in pericentromeric regions at the P=0.01 level. A total of 2 704(19.9% of total) long-InDels were located in genic regions, including 319 large-effect long-InDels, which resulted in truncated or elongated protein sequences. A previously identified QTL(qP H16) underlying plant height was further analyzed, and it was found that 26 out of 35(74.3%) long-InDel markers located in the qPH16 region showed clear polymorphisms between parents ZP and ZH. Seven markers, including three long-InDels and four previously reported SNP markers, were used to genotype 242 recombinant inbred lines derived from ZP×ZH. As a result, the qPH16 locus was narrowed from a 960-kb region to a 477.55-kb region, containing 65 annotated genes. Therefore, these long-InDels are a complementary genetic resource of SNPs and short-InDels for plant height and can facilitate genetic studies and molecular assisted selection breeding in soybean.
基金the National Key R&D Program for Crop Breeding(2016YFD0100201)Scientific Innovation Program of of the Chinese Academy of Agricultural Sciences(CAAS)。
文摘In order to screen the aroma characteristics of soybean,a new method was established which can quickly quantify the content of 2-acetyl-1pyrroline(2-AP),an important compound related to soybean aroma,using gas chromatography-mass spectrometry(GC-MS).Based on peak profile,total peak area and retention time as test indexes,an accurate identification method for fragrant soybeans was established.The optimum parameters of the protocol consisted of column temperature70℃,sample injector temperature 180℃,optimum extraction alcohol content 1 m L,Na Cl content 0.1 g,ultrasonication time10 min,and extraction time 1 h,which were established by using the orthogonal test of single factors and three factors with four levels(L_9(3)^4).2-AP content of leaves had significant correlations with seeds,which were easier to measure.The protocol was simple and easy to carry out,consumed only small amounts of reagents,and provided accurate and reliable results with good reproducibility.A total of 101 soybean genotypes from different geographical sources were analyzed using this protocol.The results showed that the average content of 2-AP was 0.29 mg L^(–1),ranging from 0.094 to 1.816 mg L^(–1),and the genetic diversity index was 0.54.Among all genotypes-tested,they were classified into three grades,including seven elite genotypes identified as"grade one fragrant soybeans",which were Zhonglong 608,Heinong 88,Ha13-2958,Hongmiandou,Heinong 82,Huangmaodou,and Jiyu 21.These results provide both an identification technique and several elite aroma genotypes for gene discovery and good quality breeding in soybean.
